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All Journal Journal of Global Pharma Technology Obat: Jurnal Riset Ilmu Farmasi dan Kesehatan
Zainab Adil Ghani Chabuck
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Health Professions Medicine & Pharmacology Nursing Public Health

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Salmonella enterica Serovar Enteritidis and Typhymurium: Phenotypic, Molecular Detection and Sequencing of Quorum Sensing Zainab Adil Ghani Chabuck
Journal of Global Pharma Technology Volume 12 Issue 06 (2020) June 2020
Publisher : Journal of Global Pharma Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (516.248 KB)

Abstract

Bacterial messaging and chatting, or Quorum sensing (QS) is a way that permitting the coordination behaviors of groups between many common bacterial pathogens. Objectives: Phenotypic detection of Quorum sensing production followed by Molecular Detection and Sequencing of its gene sdiA. Materials and Methods: A primers pair for PCR detection of sdiA gene of Salmonella enterica serovar enteritidis and typhymurium had been designed for its detection and followed by its sequencing for detection of mutation using automated sequencing. These were preceded by phenotypic detection of QS. Results: Quorum sensing produced by S. enterica was studied. Results revealed that homoserine lactone production causes the appearance of bacterial cells aggregation, which appears best after 4hours of incubation where it is representing a maximum concentration of homoserine lactone. In addition, sdiA gene is present in all isolates. Sequencing of sdiA gene from isolates of Salmonella enterica serovar enteritidis and typhymurium propose that there were 8 mutations in three isolates, also gave identity in a percentage of (98-99%) with standard strand according to NCBI web site. Keywords: Quorum sensing, Salmonella enterica, sdiA, sequencing.
An In Vitro Usage of Origanum majorana Watery Extract as Inhibitory Antibacterial Component against Different Medically Important Bacterial Isolates Zainab Adil Ghani Chabuck
OBAT: Jurnal Riset Ilmu Farmasi dan Kesehatan Vol. 3 No. 2 (2025): March: OBAT: Jurnal Riset Ilmu Farmasi dan Kesehatan
Publisher : Asosiasi Riset Ilmu Kesehatan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61132/obat.v3i2.1083

Abstract

Origanum majorana demonstrates a broad spectrum of effectiveness against various diseases, exerting antimicrobial effects against different pathogenic microorganisms. The goals of this research are to provide light on how Origanum majorana extract inhibits human pathogenic bacteria, how it may prevent biofilm development, and how it affects bacterial adhesion. The antibacterial activity of the water-based Origanum majorana extract was assessed in this research using two different methodologies. "Antibiotics were compared to its effectiveness using an agar-well diffusion assay and disc diffusion method," the first step. Additionally, the extract's ability to inhibit biofilm formation and bacterial adherence was assessed through specialized tests. All bacterial isolates of Gram negative, Gram-positive bacterial types were sensitive to Origanum majorana extract and the range of inhibition zone (30 to 24) mm. Although floxacin was effective against some of these isolates, the majority of them were resistant. Most bacterial Gram negative types were exhibit moderate adherence and biofilm activity to this extracts and some bacterial isolated of bacteria were exhibit high adherence and biofilm activity to the watery extracts of Origanum majorana. This research confirms previous findings that Origanum majorana extracts are very effective against a wide variety of clinical isolates of bacteria, including Gram-negative as well as Gram-positive varieties. Notably, the extracts were found to be more effective than commercially available antibiotics. Furthermore, the extracts displayed significant inhibition of bacterial adherence and biofilm formation. Based on these findings, Origanum majorana extracts have great promise as an effective antibacterial and anti-biofilm agent.
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