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Silvia Werdhy Lestari
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Medicine & Pharmacology

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A Concise Review of Spermatogenic Cells Culture: Assessing the Apoptotic Pathway Silvia Werdhy Lestari
Journal of Global Pharma Technology Volume 12 Issue 01
Publisher : Journal of Global Pharma Technology

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In the absence of sperm retrieved from azoospermic male, thus considered in utilizing developed spermatogenic cells including spermatogonia to spermatid in restoring male fertility. Spermatogenic cells obtained from testicular tissue culture need to be expanded prior its utilization, yet the excessive apoptosis of resulting cell culture is a challenge. This review was aimed to discuss article dealing with the factors and the underlying mechanism inducing apoptosis of spermatogenic cells culture. A computerized search of PubMed and google scholar database was conducted for general terms such as “male infertility”, “azoospermia”, “human spermatogenesis, “spermatogenic cells” and “apoptosis” and followed by screening and evaluation of collected paper accordingly. Various agents induced the apoptosis in spermatogenic cells such as high temperature, hypothermic storage, metabolite of cell culture derived and gonadotropin suppression driving to apoptosis. Thus all of the agents were paving the specific pathways of inducing apoptosis, through extrinsic and intrinsic, leading to the activation of caspase initiators followed by the activation of caspase executor in various types of spermatogenic cells.Keywords: Human spermatogenic cells, Apoptosis, Culture.
Expression of DAZL and BOULE in Spermatogenic Failure of Infertile Men: A Study Silvia Werdhy Lestari
Journal of Global Pharma Technology Volume 11 Issue 04.
Publisher : Journal of Global Pharma Technology

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Abstract

DAZ-like (DAZL) on chromosome 3 and BOULE on chromosome 2 are genes included in the DAZ gene family. These genes have a role as regulator of specific cell cycle in germ cells. Mutations on DAZ gene family cause meiotic arrest and infertility. DAZL and BOULE are known to have interaction with CDC25 in regulating meiosis in the cell cycle. The examination of infertility on azoospermia cases, which are caused by spermatogenesis arrest, has so far been limited by histological examination from testicular biopsy. Therefore, molecular research is needed to determine the candidate genes that could be used as a marker in improving the quality of testicular biopsy examination. This research is a cross-sectional study which used 40 biopsy testes samples based on Johnsen scores 2 to 8. The mRNA expression analysis of DAZL and BOULE used qRT-PCR. The DAZL gene began to be expressed in scoring 5-8, where there was an increase from scoring 5-7 then a decrease in scoring 8. The BOULE gene began to be expressed in scoring 2 – 8, but there was an increase from scoring 4 – 7 and then a decrease in scoring 6 and 8. The correlation between the mRNA expressions of DAZL and BOULE showed a significant positive correlation (r = 0.415, p<0.05). These results indicated that both DAZL and Boule contribute in the failure of spermatogenesis.Keywords: Male infertility, Spermatogenic failure, Johnson score, DAZL, BOULE
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