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Raed M. Hussein
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Medicine & Pharmacology

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Spectrophotometric Determination of Atenolol via Oxidative Coupling Reaction with 2, 4 - Dinitrophenyl Hydrazine Raed M. Hussein
Journal of Global Pharma Technology Volume 11 Issue 09: (2019) September 2019
Publisher : Journal of Global Pharma Technology

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Abstract

 An correct and sensitive spectrophotometric methodology has been developed for the determination of beta-adrenergic blocker (ATN) in pure and dose forms. the strategy is predicated on the oxidization of 2,4-dinitrophenylhydrazine (2,4-DNPHz) by metallic element per iodate than coupling with beta-adrenergic blocker (ATN) in a calescent  medium  to create actable, Yellow colored  , soluble dye with a most absorption at 403 nm. The variables that have an effect on the completion of reaction are fastidiously optimized. Beer’s law is obeyed over the concentration vary of (2-30 μg.mL-1) with molar absorption factor of (3.515×104 L.mol-1.cm1).The limit of detection was (0.07575μg.mL-1) and Sandal's sensitivity price was 0.0350 μg.cm-1. The projected methodology has been applied with success to the determination of antihistamine in pharmaceutical preparation.
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