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Zahraa Hameed Oda Alquraishi
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Medicine & Pharmacology

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Genotype and Phenotype Detection of E. coli Isolated from Children Suffering from Urinary Tract Infection Zahraa Hameed Oda Alquraishi
Journal of Global Pharma Technology Volume 10 Issue 01: (2018) Jan. 2018
Publisher : Journal of Global Pharma Technology

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Abstract

One hundred clinical urine samples were obtained from patients suffering from UTI during the period from 1, August, 2017 to 9, October, 2017. Methodology: The identification of the E. coli isolates was depended on colonial morphology, microscopic examination and biochemical tests as a primary identification. The final identification was performed by PCR technique with Mdh. In addition antibiotic genes were used such as (bla-CTM, blatem-1, aac (6) - Ib-crandsul-1). Result: Rendering  to  the  results  obtained  according to the morphological ,cultural and biochemical characters from 100 sample  only   80 (80%) isolate was  identify as  E. coli. According  to  the  results  obtained  by  PCR, Mdh gene  that  encode  was found in 56 (70%) isolates in  E. coli. The results of PCR revealed that the percentage of bla-CTX-M gene among tested isolates reached to 30/56(53.57%), 10/56(17.85%)   for blaTEM-1, 13/56(23.2%) for aac (6’)-Ib –cr and 33/42 (78.57%) for Sul-1gene. Conclusion: The  study  investigated  the  antibiotic resistance genes  of  E. coli which  play a major  role  in pathogen city .Keywords: E. coli, Urinary tract infection and antibiotic resistance genes.
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