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Ali Jaffar Saleem
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Medicine & Pharmacology

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High Frequency of Hemolysin Associated Genes among Staphylococcus Aureus Clinical Isolates in Iraq Ali Jaffar Saleem
Journal of Global Pharma Technology Volume 09 Issue 12
Publisher : Journal of Global Pharma Technology

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AbstractIn current study, 120 clinical isolates of Staphylococcus aureus were collected from different hospitals in Diyala. In addtion of phenotypic method, all isolates diagnosed as S. aureus by genotypic method was done by Polymerase Chain Reaction (PCR) technique to detection nuc gene. PCR technique has been used to investigate hemolysin associated genes that encoding for hla, hlb, hlg and hld by using specific primers which showed the prevalence in percentage (95%, 79.17%, 90% and 96.67%) respectively. All isolates were found to be positive for at least two or more hemolysin genes and 69.17% of isolates were carrying all hemolysin genes.Keywords: Staphylococcus aureus, Thermo nuclease gene, Virulence genes, Alpha, Beta, Gamma and delta hemolysin.
Study of Evolution of Multi Drug Resistance Protues spp. Isolated from Patient Suffering from UTI Ali Jaffar Saleem
Journal of Global Pharma Technology Volume 11 Issue 2: 2019
Publisher : Journal of Global Pharma Technology

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Clinical isolates of proteus spp. (30 isolates) were obtained from patients suffering from UTI from Baghdad Medical City hospital during the period between 1st December/2016 till 1st April 2017. The isolates were identified by culturing on MacConkey agar and blood agar then diagnosed by performing some morphological and biochemical tests. Proteus spp distributed in this study to P.mirabilis 23(76.6%) and P.vulgaris 7 (23.3%). In this study 17/30 (56.6%) of the sample were isolates from female and 13/30 (43.3%) isolates from male. Antibiotic susceptibility test for three antibiotics was performed using disc diffusion method. The percentage of highest resistance in proteus spp. isolates reached 80% (24/30) for Ampecillin and 76.6% (23) for Piperacillin while lower reached 50% (15/30) to Imipenim. Detect the resistance rates according to the sex of the patients showed significant differences for resistance to Imp in female 33.3% (10/30) compared with 16.6% (5/30) in male and for Amp, Pip in male 33.3% (10/30), 33.3% (10/30) compared with 46.6% (14/30), 43.3% (13/30) in female respectively.
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