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Mohammed F. Al- Marjani
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Medicine & Pharmacology

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Biofilm Formation in Imipenem-Resistant Acinetobacter baumannii from the Intensive Care Unit Mohammed F. Al- Marjani
Journal of Global Pharma Technology Volume 9 Issue 10
Publisher : Journal of Global Pharma Technology

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Acinetobacter baumannii is emerging as an important hospital pathogen, which can persist in the environment for extended periods of time. It is known to produce biofilms, a community of bacteria enclosed within a protective polymeric matrix. One hundred A. baumannii isolates were collected from patients from the intensive care unit (ICU). Antimicrobial susceptibility test was performed by disk diffusion method and MICs of imipenem were determined. Biofilm formation was assayed by micro titer plate and then PCR was performed for detection of ompA and bap genes. The results show that the imipenem-resistance was seen in 80% of A. baumannii isolates, and all these isolates were inferred to be (MBLs) producers. Among these isolates, 91.25% formed biofilms, 32.5% of isolates were categorized as moderately biofilm-producing isolates, 11.25% formed strong biofilms, and 8.75% of the isolates were non-biofilm producers. All isolates simultaneously carried the ompA gnes, whereas 70% of isolates harbored bap gene. Our results revealed a high prevalence of Metallo Beta-Lactamase and biofilm formation in Imipenem -resistant A. baumannii isolates from ICU.Keywords: Acinetobacter  baumannii, Imipenem -resistance, Biofilm, Iraq.
Long Term Survival of Pseudomonas aeruginosa in Distilled Water and in Normal Saline Mohammed F. Al- Marjani
Journal of Global Pharma Technology Volume 11 Issue 09: (2019) September 2019
Publisher : Journal of Global Pharma Technology

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Pseudomonas aeruginosa is an important opportunistic pathogen primarily causing nosocomial infections in immunocompromised patients and is responsible for high mortality rates in burn centers, it can be isolated from hospitals, from the water in intensive care units, as well as sinks, basins, drains, showers, toilets and bathtubs, leading to transmission of P. aeruginosa infections. To determine the physiological adaptations which allow P.aeruginosa to survive in aqueous environments, we performed this experiment by transfer the bacterial cultures from Luria broth to distilled water and normal saline and incubate for 6 months and 12 months respectively. P. aeruginosa was able to survive in distilled water for at least 180 days( 6 months) and in normal saline for more than 360 days(12 months). We also examined the ability of this cells to pyocyanin production and antibiotic resistance, and the results showed that P. aeruginosa  cells were able to produce pyocyanin and resist to  cefotaxime , augmentin , ceftazidime cefepime and sensitive to imipenem , norfloxacin and gentamicin. The results of this study revealed that  P. aeruginosa bacteria can be preserved in distilled water or normal saline for several months, and able to pyocyanin production as well as antibiotic resistance. It is important to understand how this bacteria survives in water. Understanding the mechanisms of survival in water may contribute to novel solutions for the prevention of P. aeruginosa infection and transmission.Keywords: Persistance, Preservation, Saline, Water.
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