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Hanan M. Ali
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Medicine & Pharmacology

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A Quantitative PCR Approach for Recognizing the Functional Genes Involved in the Degradation of Naphthalene Dioxygenase and Polycyclic Aromatic Hydrocarbons in Contaminated Soils Hanan M. Ali
Journal of Global Pharma Technology Volume 11 Issue 03
Publisher : Journal of Global Pharma Technology

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Abstract

Analysis of environmental pollutants is very important aspect; cheap and quick methods to clean-up the environment are required. Therefore, the polycyclic aromatic hydrocarbons (PAHs) and naphthalene dioxygenase (NAH-F/NAH-R) were separated from crude oil from north Al-Rumaila/ Iraq. These compounds were used in different concentrations as degraders that docking the functional genes of each of Aspergillus Niger strain AHBR3, Cladosporium ramotenellum and Penicillium brevicompactum isolate 66 small. The results were showed that the three microorganisms use these compounds as a source of food and energy to degrade the pollutants.Keywords: Pollutants, Polycyclic aromatic hydrocarbons, Aspergillus Niger, Cladosporium ramotenellum, Penicillium brevicompactum
Analytical and Theoretical Studies of Some Diazo Dyes Hanan M. Ali
Journal of Global Pharma Technology Volume 11 Issue 04.
Publisher : Journal of Global Pharma Technology

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Abstract

Analytical studies were carried on diazo dyes that characterized 4,6-bis((E)-(3-nitrophenyl)diazenyl) benzene-1,3-diol (1), 4-((E)-(3-nitrophenyl)diazenyl)-6-((E)-(4-nitro phenyl)diazenyl)benzene -1,3-diol (2), 4-((E)-(2-nitrophenyl) diazenyl)-6-((E)-(3-nitro phenyl)diazenyl)benzene-1,3-diol (3) and 4,6-bis((E)-(2-nitrophenyl) diazenyl)benzene-1,3-diol (4). The results of the solvents effects and the pH effects were showed high solubility of these dyes in ethanol and the suitable pH values were found to be in the pH 12. Three isopiestic points were gained in the results of the azo dyes (2), (3) and (4). Add to which, the ionization (pKa) and the protonation (pKb) constants were calculated by using the half height method. The results were showed that the pKa1 values were absence intheresults ofdiazo dyes (3) and (4). Theoretical studies also approved the structures of diazo dyes (1), (2), (3) and (4). These studies were focused in the relation between the structure and the properties of these molecules. Due to the internal coordinate mechanics (ICM), the conformational analysis, the molecular mechanics (MM2) and the optimal minimization were attended for each azo dye.Keywords: Azo dyes, Internal coordinates, Conformational analysis, Molecular mechanics, Ionization constant and Protonation constant.
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