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Sddiq Ghani Al-Muhanna
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Medicine & Pharmacology

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Cloning and Expression of GST-HMPREF0351_11084 Gene in JM105 Cells: A New Potential Protein Vaccine for Pneumococcal Sddiq Ghani Al-Muhanna
Journal of Global Pharma Technology .
Publisher : Journal of Global Pharma Technology

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The HMPREF0351_11084 gene encoding pneumococcal vaccine antigen A family protein (GeneID: 12999770) was optimized and synthesized, then sub-cloned into pGH cloning vector. The amplified HMPREF0351_11084 gene was incorporated into Bam HI and Eco RI sites of pGEX-2T GST expression vector under the control of tac promoter. The recombinant pGEX-2T was transformed and expressed into JM105 cells. The protein of expressing bacteria was analyzed by SDS-PAGE and the target protein was detected by Western blot - enhanced chemiluminescence (WB-ECL) assay. The result shows predicted target fusion protein with molecular weight 51.2 kDa. The current study describes for the first time the expression of HMPREF0351_11084 gene, and so attempts to produce a new potential protein vaccine for pneumococcal. Further extensive study is required to generate more data about HMPREF0351_11084 protein and evaluate its immunization in elicits antibodies that are capable of passively protecting against pneumococcal infections.Keywords: Fusion protein, PGEX-2T, HMPREF0351_11084, ECL, Pneumococcal vaccine.
Isolation and Diagnosis of Staphylococcus spp. using VITEK-2 System from Nasal and Ear of Healthy Carriers Sddiq Ghani Al-Muhanna
Journal of Global Pharma Technology Volume 11 Issue 09: (2019) September 2019
Publisher : Journal of Global Pharma Technology

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One hundred of nasal and ear swabs were collected from healthy students of Babylon University who not suffering from any upper respiratory tract infection and have not received any antibiotics from the period of two. All they were cultured on suitable media .The positivity of culturing on Mannitol salt agar (selective media for Staphylococci) was 20% distributed as 14% for nasal swabs and 6% for ear swabs. When Biochemical and Serological tests were used for identifying Staphylococcus species, the results showed that 45% of these isolates were Coagulase positive while 55% were Coagulase negative .Staphylococcus species were identified by using VITEK-2: Staphylococcus aureus 45%, S. epidermidis 20%, S.capitis sub.capitis20% and S.cohni. Sub. cohni 10% .Antibiotic sensitivity test for Staphylococcus isolates showed sensitivity for ciprofloxacin to all isolates while appeared resistant to Nalidixic acid, Kanamycin  and Gentamycine, most of Staphylococcus aureus isolates were resisted to Methicillin antibiotic. 
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