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All Journal BALI MEDICAL JOURNAL (BMJ) Journal of Stem Cell Research and Tissue Engineering Journal Orthopaedi and Traumatology Surabaya (JOINTS) Buletin Veteriner Udayana
Deya Karsari, Deya
Stem cell Research and Development Center, Airlangga University, Surabaya-Indonesia
Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Chondrogenic Differentiation Capacity of Human Umbilical Cord Mesenchymal Stem Cells with Platelet Rich Fibrin Scaffold in Cartilage Regeneration (In Vitro Study) Sumarta, Ni Putu Mira; D, Coen Pramono; Hendrianto, Eryk; Susilowati, Helen; Karsari, Deya; Rantam, Fedik A.
BALI MEDICAL JOURNAL Vol 5 No 3 (2016)
Publisher : BALI MEDICAL JOURNAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (541.428 KB)

Abstract

Background: Human umbilical cord mesenchymal stem cell is a promising source of allogenous MSC with great chondrogenic differentiation capacity. Meanwhile, platelet rich fibrin (PRF) is a natural fibrin matrix, rich in growth factors, forming a smooth and flexible fibrin network, supporting cytokines and cell migration, thus can be used as a scaffold that facilitate the differentiation of MSC. However, the differential capability of MSC cultured in PRF was still poorly understood. Method: We studied in vitro differentiation potential of MSC cultured in PRF by evaluating several markers such as FGF 18, Sox 9, type II collagen, aggrecan in 3 different culture medium. Result: The result showed that there was positive expression of FGF 18, Sox 9, type II collagen, aggrecan in all medium of in vitro culture. Conclusion: MSC cultured from human umbilical cord had the capacity of chondrogenic differentiation and able to produce cartilage extracellular matrix in vitro which means that hUCMSC is a potential allogeneic MSC for cartilage regeneration.
Isolation and Identification Avian Influenza A non-H5 Virus from Muscovy Duck (Cairina moschata) at Two Live Bird Markets in Surabaya karsari, deya
Journal of Stem Cell Research and Tissue Engineering Vol. 4 No. 1 (2020): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jscrte.v4i1.21597

Abstract

The aim of this study was to isolate and identify Avian Influenza A non-H5 virus from muscovy duck at two live bird markets in Surabaya. Muscovy duck is the natural reservoir of Avian Influenza virus, in which all of the 16 HA subtypes and 9 NA maintained. The Avian Influenza virus replicates in intestinal tract of the reservoirs, causing the high amount of virus shed in the faeces. This study is an observational descriptive study, using non random sampling method of determined samples. The  method used in this study were Hemagglutination Inhibition (HI) test and Enzyme Linked Immunosorbent Assay (ELISA) test. Avian Influenza A non-H5 virus was identified 19.23% (5 samples out of 26) in PS1 and  23.34% (7 samples out of 30)  in PS2. This finding shows that  Avian Influenza A non-H5 virus could be isolated and identified from muscovy duck at two live bird markets in Surabaya.
OPTIMIZATION OF ISOLATION, PURIFICATION, AND FREEZE-DRYING FOR BOVINE PLATELET-RICH PLASMA (PRP) TO DEVELOP BIOLOGICAL PRODUCT Ihsan, Igo Syaiful; Fauziah, Ima; Hendrianto, Eryk; Karsari, Deya; Syah, Alif Firman; Qomar, Nadira Virgin Al
Buletin Veteriner Udayana Bul. Vet. Udayana. February 2026 Vol. 18 No. 1
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2026.v18.i01.p19

Abstract

Platelet-rich plasma (PRP) is an autologous plasma fraction enriched with platelets and growth factors that support tissue repair. In veterinary applications, bovine PRP shows therapeutic potential, but its clinical use is limited by the short stability of fresh preparations. Freeze-drying offers a promising method to improve PRP shelf-life and usability. This study aimed to develop a standardized protocol for bovine PRP isolation, characterization, and freeze-drying. Blood samples (400 mL) were collected from six healthy cows using CPDA-1 anticoagulant. Hematological screening was performed using an automated analyzer. PRP was isolated via a double-spin centrifugation protocol and subsequently lyophilized using a controlled freezing and freeze-drying system. Data were analyzed using descriptive statistics, Shapiro-Wilk normality tests, and Spearman correlations. Hematology profiles showed wide biological variation in WBC, HGB, and HCT, while RBC, MCV, and PLT remained within stable ranges. Double-spin centrifugation effectively increased platelet concentration up to threefold from baseline. Freeze-drying produced stable PRP powder with low residual moisture, suitable for storage at room temperature. Significant positive correlations were found among RBC-HGB-HCT, reflecting normal erythropoietic physiology. The developed protocol successfully standardized bovine PRP isolation and freeze-drying procedures, producing stable and concentrated PRP suitable for extended storage. Freeze-dried bovine PRP has strong potential as a practical biological product for veterinary regenerative applications and cattle health management.Further studies are recommended to evaluate the biological stability and clinical efficacy of freeze-dried bovine PRP in various veterinary therapeutic applications.
Hypoxic Culture (1% O2) Preserves Stemness and Reduces Spontaneous Osteogenic Differentiation In Early and Late Passage Bone Marrow-Derived Mesenchymal Stem Cells Rahadiansyah, Erreza; Mubarok, Muhammad Iqbal; Karsari, Deya
(JOINTS) Journal Orthopaedi and Traumatology Surabaya Vol. 15 No. 1 (2026): April 2026
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/joints.v15i1.2026.1-9

Abstract

Background: Bone marrow–derived mesenchymal stem cells (BMMSCs) are widely investigated for regenerative medicine applications. During in vitro expansion, BMMSCs may undergo loss of stemness and spontaneous lineage commitment. Hypoxic culture conditions better mimic the physiological bone marrow niche; however, their effects across early and late passages remain incompletely understood. This study aimed to evaluate the effect of hypoxic culture (1% O₂) on stemness-associated features, BMMSC identity, and spontaneous osteogenic mineralization at early and late passages.Methods: BMMSCs were isolated from a New Zealand White rabbit and cultured under normoxic (21% O₂) or hypoxic (1% O₂) conditions. Cells at passages 4 and 8 were analyzed for OCT4, SOX2, and CD105 expression using immunofluorescence and immunocytochemistry. Osteogenic differentiation was assessed by Alizarin Red S staining. Statistical analysis used independent t-tests and Mann–Whitney U tests (p < 0.05).Results: SOX2 and CD105 expression significantly decreased at late passages compared to early passages under both conditions (p < 0.05), whereas OCT4 expression remained stable under hypoxia (p > 0.05). BMMSCs cultured under hypoxia exhibited significantly higher expression of OCT4, SOX2, and CD105 than normoxic cultures at both passages (p < 0.05). Spontaneous calcium deposition was significantly lower under hypoxia (p < 0.05), with no significant difference between early and late passages.Conclusion: Hypoxic culture conditions better preserve stemness-associated features and MSC identity in BMMSCs and suppress spontaneous osteogenic mineralization. Prolonged hypoxic culture supports the maintenance of stem-like characteristics during in vitro expansion, highlighting its relevance for optimizing BMMSC culture strategies.
Co-Authors Eryk Hendrianto Fauziah, Ima Fedik A. Rantam, Fedik A. Helen Susilowati, Helen Ihsan, Igo Syaiful Mubarok, Muhammad Iqbal Ni Putu Mira Sumarta Qomar, Nadira Virgin Al Rahadiansyah, Erreza RM Coen Pramono D Syah, Alif Firman