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All Journal BALI MEDICAL JOURNAL (BMJ) Journal of Stem Cell Research and Tissue Engineering
Deya Karsari, Deya
Stem cell Research and Development Center, Airlangga University, Surabaya-Indonesia
Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Veterinary

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Chondrogenic Differentiation Capacity of Human Umbilical Cord Mesenchymal Stem Cells with Platelet Rich Fibrin Scaffold in Cartilage Regeneration (In Vitro Study) Sumarta, Ni Putu Mira; D, Coen Pramono; Hendrianto, Eryk; Susilowati, Helen; Karsari, Deya; Rantam, Fedik A.
BALI MEDICAL JOURNAL Vol 5 No 3 (2016)
Publisher : BALI MEDICAL JOURNAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (541.428 KB)

Abstract

Background: Human umbilical cord mesenchymal stem cell is a promising source of allogenous MSC with great chondrogenic differentiation capacity. Meanwhile, platelet rich fibrin (PRF) is a natural fibrin matrix, rich in growth factors, forming a smooth and flexible fibrin network, supporting cytokines and cell migration, thus can be used as a scaffold that facilitate the differentiation of MSC. However, the differential capability of MSC cultured in PRF was still poorly understood. Method: We studied in vitro differentiation potential of MSC cultured in PRF by evaluating several markers such as FGF 18, Sox 9, type II collagen, aggrecan in 3 different culture medium. Result: The result showed that there was positive expression of FGF 18, Sox 9, type II collagen, aggrecan in all medium of in vitro culture. Conclusion: MSC cultured from human umbilical cord had the capacity of chondrogenic differentiation and able to produce cartilage extracellular matrix in vitro which means that hUCMSC is a potential allogeneic MSC for cartilage regeneration.
Isolation and Identification Avian Influenza A non-H5 Virus from Muscovy Duck (Cairina moschata) at Two Live Bird Markets in Surabaya karsari, deya
Journal of Stem Cell Research and Tissue Engineering Vol. 4 No. 1 (2020): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jscrte.v4i1.21597

Abstract

The aim of this study was to isolate and identify Avian Influenza A non-H5 virus from muscovy duck at two live bird markets in Surabaya. Muscovy duck is the natural reservoir of Avian Influenza virus, in which all of the 16 HA subtypes and 9 NA maintained. The Avian Influenza virus replicates in intestinal tract of the reservoirs, causing the high amount of virus shed in the faeces. This study is an observational descriptive study, using non random sampling method of determined samples. The  method used in this study were Hemagglutination Inhibition (HI) test and Enzyme Linked Immunosorbent Assay (ELISA) test. Avian Influenza A non-H5 virus was identified 19.23% (5 samples out of 26) in PS1 and  23.34% (7 samples out of 30)  in PS2. This finding shows that  Avian Influenza A non-H5 virus could be isolated and identified from muscovy duck at two live bird markets in Surabaya.
Co-Authors Eryk Hendrianto Fedik A. Rantam, Fedik A. Helen Susilowati, Helen Ni Putu Mira Sumarta RM Coen Pramono D