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Stability of Anti-Insect Ingredient from Jayanti Plants (Sesbania sesban) for Integrated Control of Cabbage Pest Suripto Suripto; Hilman Ahyadi; Rachmawati Noviana Rahayu; Lalu Japa
Jurnal Penelitian Pendidikan IPA Vol 9 No 2 (2023): February
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i2.3293

Abstract

The specific purpose of this study was to determine the stability of S. sesban insecticide during storage before application and its stability in water during application. The dry powder of S. sesban leaves was extracted using water as the single solvent. Bioassay of S. sesban leaf extract was carried out on cabbage caterpillars (Plutella xylostella larvae) and Diadegma semiclausum imago on cabbage plants using a variation of storage time design. Mortality data of P. xylostella larvae and D. semiclausum imago were respectively processed by probit analysis to determine LC50. The results showed that the insect repellent content of S. sesban leaves was a compound from the saponin group and was unstable during simplicia storage, extract storage and also unstable in water before and during application. Storage of S. sesban leaf simplicia from 1 to 3 months only slightly reduced the lethal toxicity of the extract to P. xylostella larvae (mortality from 95 to 80% or LC50 from 28.82 to 28.83 ppm), but after 6 to 12 months storage, the lethal toxicity decreased drastically (mortality was 12.5 to 1.25% and LC50 was 247.99 ppm until calculated). Storage of S. sesban leaf extract from 7 to 15 days had resulted in a sharp decrease in lethal toxicity to P. xylostella larvae (mortality 70 to 40% and LC50 34.05 to 59.43 ppm) and 30 days storage causes the insect repellent to be inactive. (mortality was only 1.25% and LC50 was unaccounted for). Exposure to a solution of S. sesban leaf extract for 24 to 48 hours caused a decrease in lethal toxicity to P. xylostella larvae (mortality 32 to 28% and LC50 62.63 to 64.85 ppm) and after the solution was stored for 72 hours, the insect repellent was almost no active again (mortality was only 1.25 and LC50 was unstoppable). All storage treatments of insect repellent from S. sesban leaves showed no significant difference in the effect of lethal toxicity on D. semiclausum imago. In all the results of the bioassays, the mortality of D. semiclasum was 0 to 1.25% and each of all LC50(s) was unaccounted for
Evaluation of Insecticidal Performances of Jayanti Plant (Sesbania sesban) for Integrated Control of Cabbage Caterpillar (Plutella xylostella) Suripto Suripto; Lalu Japa; Hilman Ahyadi; Rachmawati Noviana Rahayu
Jurnal Penelitian Pendidikan IPA Vol 9 No 10 (2023): October
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i10.5635

Abstract

The study on the use of local natural materials as a source of insecticides is intended, among other things, to reduce farmers' dependency on using synthetic chemicals. This research was conducted to obtain the most selective insecticidal active ingredient from Sesbania sesban plant for controlling cabbage caterpillars, choose a target mode of action that has a safe impact on the environment, and determine insecticidal stability during storage of the material prior to application and its stability in water after application.  The dried powder of S. sesban leaves was extracted in stages using a series of solvents, namely hexane, DCM, ethanol and water, respectively.  Each of the extract fractions produced was tested for its lethal toxicity against Plutella xylostella larvae and Diadegma semiclausum imago. Each of mortality data were processed by probit analysis to produce LC50(s) to determine their insecticidal selectivity. Subsequent bioassays were carried out using extract-water of S. sesban leaves and the data were processed by probit analysis to determine their anti-ovipositor, ovicidal powers and anti-feedant properties against P. xylostella. The insecticidal stability of S. sesban was also studied according to variations in the storage time of the simplicia before extraction, the storage time of the extract before dissolution, and its stability in water during application. The data was processed using ANOVA. The results show that the insecticidal activity of the ethanol extract fraction of S. sesban leaves for controlling cabbage caterpillars was very selective, namely very toxic to P. xylostella but very less toxic to D. semiclausum. Against P. xylostella, S. sesban leaf extract has also been proven to significantly prevent oviposition, inhibit egg hatching and feeding activity. The insecticidal power of S. sesban did not decrease significantly during storage of the simplicia for less than three months and during storage of the extract for less than seven days. However, the toxicity of the extract solution decreased drastically after 24 hours of application