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All Journal Jurnal MIPA Jurnal Biologi Tropis
Ika Nugraheni Ari Martiwi, Ika Nugraheni Ari
Fakultas Sains dan Teknologi UIN Sunan Kalijaga Yogyakarta, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Mathematics Physics

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Penambahan Ekstrak Pegagan (Centella asiatica) Sebagai Anti kontaminan pada Medium In Vitro Alternatif Perkecambahan Anggrek Dendrobium macrophyllum A. Rich Martiwi, Ika Nugraheni Ari; Wahyuni, Endang Sri
Jurnal MIPA Vol 5, No 2 (2016)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jm.5.2.2016.13103

Abstract

Kondisi aseptis mutlak diperlukan dalam kultur jaringan tumbuhan. Salah satu strategi untuk meminimalisasi terjadinya kontaminasi adalah dengan menambahkan senyawa antifungi dan antibakteri. Centella asiatica atau pegagan dikenal sebagai salah satu tanaman yang mengandung senyawa yang bersifat antimikrobia dan antifungi, sebagai antioksidan dan antikanker. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak pegagan yang berpotensi sebagai antikontaminan pada perkecambahan anggrek Dendrobium macrophyllum dalam medium alternatif pupuk daun dan penambahan ekstrak ragi. Penelitian ini menggunakan biji anggrek umur 4 bulan yang ditanam dalam medium VW dan pupuk daun dengan penambahan variasi bahan organik berupa ekstrak ragi dan ekstrak pegagan. Hasil Penelitian menunjukkan bahwa komposisi Media pupuk daun 1,5 g/l, ekstrak ragi 1,25 g/l, dan ekstrak daun pegagan 5 % merupakan media optimal untuk perkecambahan biji anggrek Dendrobium macrophyllum karena dapat bertahan aseptis, memiliki waktu inisiasi biji berkecambah tercepat, jumlah prosentase perkecambahan terbanyak dan warna protocorm hijau segar. Aseptic conditions of explants is absolutely necessary in plant tissue culture. One of strategies to minimize contamination is adding antimicrobial compounds. Centella asiatica is known as one of the plants containing compounds with antioxidant, anticancer, antibacterial and antifungal properties. This study aimed to determinate the effect of C. asiatica extracts as an anti-contaminant on orchid germination medium. Four months old orchid seeds were planted in medium foliar fertilizer and yeast extract supplemented by C. asiatica extract with various concentration and medium VW as a control. The results showed that the optimum media for orchid seeds germination consisted of 1,5 g/l of foliar fertilizer, 1,25 g/l of yeast extract, 5% of C. asiatica extract based on germination time, the number of protocorm and the fresh green colour of protocorm as compared with other treatments.
Morphological characters of Species Members of the Solanaceae Family in Menoreh Samigaluh Hill, Yogyakarta Luthfika, Muhammad; Martiwi, Ika Nugraheni Ari
Jurnal Biologi Tropis Vol. 24 No. 1 (2024): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i1.6298

Abstract

The Solanaceae family has 1,300 genera distributed in the sub-tropics and tropics. In Indonesia, the genus of the Solanaceae family is used as a source of food and medicine. Solanaceae as plants used for food, medicine and health care, require a precise and accurate identification process. Automatic plant identification can use visual morphological characteristics. Environmental conditions such as extreme temperatures, drought, and water stress cause plants to adapt. The adaptation process can induce morphological changes, this study aims to study the morphological characters of solanaceae in the karst area, the hills of the menorah samigaluh Yogyakarta. The data collection method uses Visual Encounter Census (VES) by purposive sampling. Data analysis was carried out in a qualitative descriptive manner. The results obtained three genera, and 7 species (Solanum melongena, Solanum torvum, Solanum lycopersicum, Solanum diphyllum, Capsicum annuum, Capsicum frutescens, and Physalis angulata). There are 23 morphological characters used as a reference in identifying species of Solanaceae members.
Diversity of Bryophytes Based on Substrate Types in the Karangkamulyan Tourist Area, Ciamis Regency Wahyuningsih, Endang Prayudaty; Martiwi, Ika Nugraheni Ari
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.8495

Abstract

Indonesia is renowned as a megabiodiversity country with a wealth of flora, including the diversity of bryophytes. These plants play a crucial role in ecosystems, such as oxygen production, erosion control, and water absorption. This study aims to analyze the diversity of bryophytes in the Karangkamulyan Tourism Area, Ciamis Regency, and to examine their diversity based on substrate types. Karangkamulyan is a cultural and natural conservation area covering 25.5 hectares with a climate and conditions that support bryophyte growth. The research utilized an exploratory method with random sampling at three selected points within the area. Morphological and anatomical analyses were conducted to identify species, and the percentage of occurrence was analyzed based on substrate types. The results revealed the presence of 15 species belonging to 12 families of bryophytes inhabiting various substrates such as soil, rocks, and tree trunks. The identified bryophytes included 6 liverworts (Marchantiophyta): Dumortiera hirsuta, Marchantia emarginata, Riccia gangetica, Heteroscyphus argutus, Heteroscyphus coalitus, and Schiffneriolejeunea sp.; and 9 mosses (Bryophyta): Neckeropsis undulata, Thuidium plumulosum, Ectropothecium falciforme, Fissidens sp., Fissidens flaccidus, Calymperes sp., Hyophila sp., Barbula sp., and Bryum sp. Based on substrate types, 91 occurrences were recorded, consisting of 25% terrestrial bryophytes (23 occurrences), 55% epiphytic bryophytes (50 occurrences), and 20% epilithic bryophytes (18 occurrences).
Co-Authors Luthfika, Muhammad Muhammad Rivai Wahyuningsih, Endang Prayudaty