Article Per Year (5 Year)
p-Index From 2020 - 2025
0.408
P-Index
This Author published in this journals
All Journal Indonesian Journal of Cancer Chemoprevention Gunung Djati Conference Series
Resti Gradia Dwiwina
Universitas Padjadjaran
Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Economics, Econometrics & Finance Education Energy Engineering Environmental Science Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Mathematics Medicine & Pharmacology Other

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Efektivitas dan Pengaruh Kombinasi Anestesi Ketamine-Xylazine pada Tikus (Rattus norvegicus) Ita Krissanti; Rifaati Hanifa; Resti Gradia Dwiwina
Gunung Djati Conference Series Vol. 18 (2023): Prosiding Seminar Nasional Biologi (SEMABIO) Tahun 2022
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (580.581 KB)

Abstract

Anesthesia is used in several studies to reduce or eliminate pain and suffering in experimental animals. One of the most widely used experimental animal species in research is rat (Rattus norvegicus). Ketamine-xylazine (KX) is a combination that is often used for injection anesthesia in rats. The properties of the KX combination ideally can induce the desired effect, such as analgesics, muscle relaxation, and sedation. However, the KX combination can cause side effects that affects the physiological function in rat’s body. This literature study discusses the variety of ketamine-xylazine combination doses that can be used as anesthesia in rats, their effect on cardiorespiratory parameters and body temperature in rats. It can be concluded that the combination of ketamine-xylazine provides a variety of doses that produce a fast onset of action and a duration of action that can be adjusted according to needs.
Cytotoxic and Anti-proliferative Effects of Ethanol Extract of Marine Sponge Stylissa carteri on Colon Cancer HCT-116 Cell Line Resti Gradia Dwiwina; Sarah Sabrina; Yuni Susanti Pratiwi; Putri Halleyana; Adrikni Rahman; Muhammad Hasan Bashari
Indonesian Journal of Cancer Chemoprevention Vol 14, No 1 (2023)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev14iss1pp33-38

Abstract

Colorectal cancer is one of the most diagnosed cancers in the world. KRAS mutations in colon cancer are being responsible for the progressiveness and resistance of the standard therapeutic available. Marine sponge is one the sources for chemotherapy. Stylissa carteri is a marine sponge that lives in Indonesia and its anti-cancer effects are starting to be explored nowadays. The purpose of this study was to determine the cytotoxicity and anti-proliferative effect of ethanol extract of Stylissa carteri against colon cancer cells with KRAS mutations HCT-116 cells. This was an in vitro study using colon cancer cell line HCT-116 which was tested by 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) and trypan blue assay. The trypan blue assay was performed on control and treated cells at 72 h. The MTT assay was performed on cells which were previously incubated with Stylissa carteri extract. Sponge Stylissa carteri was taken from Pramuka island, Taman Nasional Kepulauan Seribu, Jakarta. The IC50 of ethanol extract of Stylissa carteri is 5 μg/mL (R square 0.9550) in HCT-116 cells. The trypan blue assay showed doubling time of incubated cell for 48 h was 26.10 h for control and 39.69 h for treated cells, respectively. Ethanol extract of Stylissa carteri has cytotoxic and anti-proliferative effects in HCT-116 colon cancer cells with KRAS mutation.Keywords: Colon Cancer, HCT-116, KRAS Mutation, Marine Sponge, Stylissa carteri.
Co-Authors Adrikni Rahman Ita Krissanti Muhammad Hasan Bashari Putri Halleyana Rifaati Hanifa Sarah Sabrina Yuni Susanti Pratiwi