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All Journal The Journal of Experimental Life Sciences (JELS)

Agung Pramana Warih Marhendra

Brawijaya University

Author-ID : 5927173

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience

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Articles

Detection of Reproductive Status in Ongole Crossbred (PO) Cow Based On Vaginal Epithel Morphology and Profile Hormone Yeni Widyaningrum; Aulanni'am Aulanni'am; Agung Pramana Warih Marhendra
The Journal of Experimental Life Science Vol. 10 No. 1 (2020)
Publisher : Postgraduate School, Universitas Brawijaya

Hormonal fluctuations in livestock will affect vaginal cytology good overview on the condition of estrous until pregnancy. The purpose of this study was to determine the physiological condition of Ongole crossbred (PO) cow during estrous and determine pregnancy by the description of vaginal epithelial cells, progesterone, and estrogen hormone profiles. The materials were used 35 cows with physiological status (estrous, 5th pregnancy period, 16th pregnancy period, 22nd pregnancy period, and 60th pregnancy period). Samples of Vaginal smear were stained with Giemsa, then it was observed using a microscope, with 40 times magnification. The progesterone and estrogen were analyzed by the ELISA method. The parameters measured were the percentage of vaginal epithelial cells, such as (parabasal, intermediate, and superficial) started estrous phase until the time of pregnancy in cows (5, 16, 22, and 60 days), hormone concentration, as well as the presence or absence of leukocytes. The result showed the Ongole crossbred cow estrous phase percentage of superficial cells 56.27%±6.49 higher than 26.23%±7.98 intermediate cells, followed by parabasal cells 17.50%±4.74. While in Ongole crossbred that were 5th pregnancy period until the 60th predominantly intermediate cell 80.43%±1.31, then the superficial cells 18.09%±1.30 and 1.48%±0.04 parabasal cells. Progesterone concentration was 63.74±1.07 ng.mL-1 in estrus cows, and steadily increased 93.71±0.94 ng.mL-1 to 149.5±0.71 ng.mL-1 in pregnant cows (5-60 days). The concentration of high estrogen levels were 122.38±0.63 ng.mL-1 in the estrous phase, then decreased 81.54±0.44 ng.mL-1 in the pregnancy phase. In conclusion, the concentration of hormone showed a diagnosis of pregnancy, which done by looking at changes in vaginal epithelial cells at the Ongole crossbred cow, and the cow estrous phase showed greater superficial cells compared by pregnant cows (5-60 days). Keywords: diagnosis of pregnancy, estrous, hormone, Ongole crossbred of cow, vaginal cytology.

Effect of Cold Storage Time (4°C) on Malondialdehyde (MDA) Level, Motility and Viability Spermatozoa of Cyprinus carpio L. Punten Strain Rosyi Wirastuti; Agung Pramana Warih Marhendra; Jantje Wiliem Souhaly; Sri Rahayu
The Journal of Experimental Life Science Vol. 10 No. 2 (2020)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2020.010.02.08

The aim of this research is to know the level of MDA, motility, and viability spermatozoa of C. carpio L. in cold storage time at 4°C. This study used treatment extenders that were NaCl 0.9% as control, diluent of egg yolk with concentrate 0%, 5%, 10%, 15%, and storage in 0 h, 24 h, 48 h, 72 h, and 96 h at 4°C. The data were analyzed using ANOVA (P<0.05). The results showed that the quality (motility and viability) of spermatozoa C. carpio L. punten strain in cold storage could be maintained at 96 h. The optimum storage for motility was found in diluent of egg yolk concentrate 5% at 48 h. MDA levels of semen from C. carpio L. Punten strains after being stored at cold temperatures increased at 0 h to 24 h, then decreased after 48 h of storage and increase at 72 h - 96 h stored. Egg yolk concentration and storage time had a significant effect on MDA levels. There is a negative correlation between MDA levels and motility. There was no correlation between MDA levels and the viability of spermatozoa C. carpio L. punten strain. Keywords: C. carpio L, Egg yolk, Malondialdehyde, Motiliy, Viability

Minnow Trap Color Effectiveness Test Using Cat Food Bait as Aquatic Sampling Gear on Diurnal Fish in Gajah Mungkur Reservoir, Cental Java, Indonesia Adityas Arifianto; Elsa Mufti; Agung Pramana Warih Marhendra; Nia Kurniawan
The Journal of Experimental Life Science Vol. 11 No. 1 (2021)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2021.011.01.04

The Minnow Trap is a simple sampling gear, efficient, easy to operate, affordable, easy to make, and has been used in research in the fisheries and aquatic ecology for more than 90 years. The brightness, color, and visual obstruction of fishing gear or fish traps affect the behavioral response of target fish, considering that each fish has a specific level of color sensitivity. The color effectiveness test of fishing gear is an important prerequisite for the use of sampling tools, and it needs to be evaluated to understand the gear performance and avoid potential sampling bias. The purpose of this study was to test the color effect of the minnow trap on diurnal fish in the Gajah Mungkur Reservoir waters. The research was carried out in Gajah Mungkur Reservoir, Wonogiri Regency, at two stations. The sampling conducted at 09.00 - 11.00 and 13.00 - 15.00 Indonesian West Time, where the initial survey is estimated to be the highest time for diurnal fish activity. The minnow trap used in this study is four colors vinyl-painted double funnel cylindrical minnow trap with a 5x5 millimeter mesh size and a conventional umbrella minnow trap used as control. The sampling results are then recorded on the datasheet and analyzed using Microsoft Excel. CPUE (Catch Per Effort Unit) of each unit is then calculated and statistically analyzed by Kruskal-Wallis test and Kolmogorov-Smirnov normality test through SPSS 25.0 software. The test result of five unit minnow traps from 40 installations and two hours of deployment time, 80 individuals were captured, consisted of seven species of freshwater fish. CPUE values ranking consecutively from the highest from silver units (1.375), black (1.25), green (1.125), red (0.9375), and control (0.3125). The Kruskal-Wallis analysis showed that all tested units do not have a significant difference. Keywords: Color, catch per unit effort, fish, minnow trap.

The Effect of Monosodium L-Glutamate (MSG) Treatment for Short and Long Terms to The Semen Quality of Adult Male Rats Ivakhul Anzila; Agung Pramana Warih Marhendra; Sri Rahayu
The Journal of Experimental Life Science Vol. 9 No. 2 (2019)
Publisher : Postgraduate School, Universitas Brawijaya

This study was conducted to investigate the effect of MSG treatment for short and long term on the semen quality of adult male rats. Twelve male adult Wistar rats with 200-300g of body weight (BW) and 3-4 month of age were used in this study. The animals were divided randomly into 3 groups. M0 was used as a control, M1 and M2 were given with MSG 4mg/gBW for 15 andÂ 45 days respectively. The experimental animals were sacrificed on the days 16th (to M1 group) and 46th (to M0 and M2 groups). The epididymis was isolated and semen quality (motility, viability, concentration, and abnormality of sperm) was evaluated. The results showed motility and concentration of M1 and M2 were not significantly decreased compared to M0. MSG treatment also significantly reduced viability and increased abnormality of sperm. Analysis of sperm abnormality character shows that the use of long-term MSG caused a formation of the primary abnormality (round and double head sperm) and increased the secondary abnormality (bent neck, curve tail, coiled tail, headless, and tailless) compared to control. Conclusion, semen quality decreases with consumed MSG for the long term. For this reason, reconsidering the use of MSG as an enhancer for the teste of food is very important.Keywords: epididymis, Monosodium L-Glutamate, semen quality.

Alcohol Intake Investigation of Adult Rats Based on Sperm Parameters Choirul Anam; Agung Pramana Warih Marhendra; Sri Rahayu
The Journal of Experimental Life Science Vol. 9 No. 2 (2019)
Publisher : Postgraduate School, Universitas Brawijaya

The prevalence of alcohol consumption is high among adult males during the reproductive period. The current study aimed to evaluate the impact of chronic administration ethanol on the quality of sperm in the rats. Twelve healthy Wistar male albino rats were randomly divided into 2 groups to represent the 2 phase duration. The first phase lasted 21 days and the second phase lasted 49 days. In each phase, the animals were separated into subgroups: A and B. Subgroup A represented control that received distilled water while subgroup B represented animals that received 7 mL.kgBW-1 of 30% ethanol per day, thrice a week. The data were analyzed using ANOVA (P<0.05). There is no significant difference in sperm concentration and viability. However, there is a significant difference in the motility of spermatozoa between the control group and ethanol-treated group. Thus, the study indicates that ethanol administration may disturb the sperm motility and have no clear effect on its concentration and viability.Keywords: alcohol consumption, reproductive function, sperm quality.

Co-Authors Adityas Arifianto Aulanni'am Aulanni'am Choirul Anam Elsa Mufti Ivakhul Anzila Jantje Wiliem Souhaly Nia Kurniawan Rosyi Wirastuti Sri Rahayu Yeni Widyaningrum

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