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Yenny Wuryandari
Innovation Center of Appropriate Food Technology for Lowland and Coastal Area, Universitas Pembangunan Nasional “Veteran” Jawa Timur, Surabaya 60294, Indonesia
Agriculture, Biological Sciences & Forestry Chemical Engineering, Chemistry & Bioengineering Economics, Econometrics & Finance Engineering Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology

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Utilization of Neem Leaves as a Biological Pesticide for the Control of Anthrachnose Diseases in Dragons (Hylocereus sp.) Nolla Dwi Elvira; Yenny Wuryandari
Nusantara Science and Technology Proceedings Seminar Nasional Agroteknologi 2022
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2023.3109

Abstract

Dragon fruit (Hylocereus sp.) has problems in its cultivation related to Plant Pest Organisms (OPT). One of the pathogens that attack dragon fruit plants is Colletotrichum sp. which can cause anthracnose. dragon fruit pest control method can be done by using biological pesticides from neem leaf extract. Pesticides from plant extracts are sources of various compounds that are rich in various types of active ingredients such as secondary metabolites that play an important role in the process of interacting or competing and protecting themselves from competitors. The purpose of this study was to determine the effectiveness of neem leaf pesticide for controlling anthracnose on dragon fruit plants. The method used is testing the inhibitory power of neem leaf biological pesticides against anthracnose on dragon fruit plants in vitro. The concentration treatments of biological pesticides included 0%, 20%, 40%, and 60%. The results obtained showed that the biological pesticide neem leaf with a concentration of 20% already had an inhibitory power against the pathogen Colletotrichum sp. which is quite good because at that concentration it already has a fairly high antifungal compound. The magnitude of the inhibitory power of neem leaf biological pesticides at a concentration of 20% was 53.94%.
Promotion of Beauveria bassiana Mushroom on Different Growing Media and Its Pathogenicity on Insects Lukmanul Hakim; Yenny Wuryandari
Nusantara Science and Technology Proceedings Seminar Nasional Agroteknologi 2022
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2023.3116

Abstract

One type of entomopathogenic fungus that is known to be effective in controlling important plant pests is the Beauveria bassiana fungus. The fungus B. bassiana, has a high reproductive capacity, is easy to produce and under unfavorable conditions can form spores that can last a long time in nature. The purpose of this study was to determine the density and viability of B. bassiana spores on corn and rice media and to investigate the process of B. bassiana fungal infection in the test insects. The method used is; Fungal propagation test on Rice and Corn Media, Microscopic Observation of Fungus from Corn and Rice Media, Calculation of Fungus Spore Density, Calculation of Fungus Viability and Pathogenicity Test. The research results obtained are as follows; Results The propagation of B. bassiana mushroom grew on the seventh day, the fungus growth was faster in rice media than in corn media. The spore density of the fungus B. bassiana clove bondowoso in corn media was 3.75x108 spores/ml, rice media was 4.58x108 spores/ml, B. bassiana banyuwangi coffee medium was 3.5x108 spores/ml, rice media was 3.75x108 spores/ml. The viability of the fungus B. bassiana, clove bondowoso and banyuwangi coffee on rice and corn media was obtained on average above 60%. The results of the pathogenicity test of B. bassiana on insects began to appear on the tenth day after the baiting process.
Analysis of Encapsulation Efficiency Bacillus sp. Based Variations in Sodium Alginate Concentration in the Beads Mochammad Mirza Saputra; Yenny Wuryandari; Noni Ramadhini
Nusantara Science and Technology Proceedings The 4th International Conference on Agriculture and Environmental Sciences (ICAES) 2023
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2024.3901

Abstract

The use of biological agents such as Bacillus sp. Bacteria have started to be widely used by farmers as a new form of control. Bacillus sp. needs to require a special method in its application because it has certain living conditions, and Bacillus sp. is susceptible to environmental stress like other microorganisms. Apply Bacillus sp. as a biological control agent in the field, it needs to be formed into a formulation to be more effective and efficient when applied. Liquid formulations often used cannot maintain the viability of bacteria in the soil. The bioencapsulation formulation in the form of microcapsules is considered more effective in maintaining the viability of bacteria in the soil. This study aims to determine the effect of concentration on the encapsulation efficiency of Bacillus sp. inside the microcapsule. Beads are made using the extrusion method by combining a sodium alginate suspension of 1%, 1.5%, and 2% concentration and a bacterial suspension and dropping it into a calcium chloride solution. The encapsulation efficiency test was carried out once by extracting the bacteria on the beads, growing them in the growth medium, and then observing them. The results of the encapsulation efficiency test with a concentration of 1%, 1.5%, and 2% had values of 1%, 0.36%, and 1.35%, respectively. The difference in the results of this encapsulation efficiency indicates that there is an effect of sodium alginate concentration on the value of encapsulation efficiency.
Utilization of Neem Leaves as a Biological Pesticide for the Control of Anthrachnose Diseases in Dragons (Hylocereus sp.) Nolla Dwi Elvira; Yenny Wuryandari
Nusantara Science and Technology Proceedings Seminar Nasional Agroteknologi 2022
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2023.3109

Abstract

Dragon fruit (Hylocereus sp.) has problems in its cultivation related to Plant Pest Organisms (OPT). One of the pathogens that attack dragon fruit plants is Colletotrichum sp. which can cause anthracnose. dragon fruit pest control method can be done by using biological pesticides from neem leaf extract. Pesticides from plant extracts are sources of various compounds that are rich in various types of active ingredients such as secondary metabolites that play an important role in the process of interacting or competing and protecting themselves from competitors. The purpose of this study was to determine the effectiveness of neem leaf pesticide for controlling anthracnose on dragon fruit plants. The method used is testing the inhibitory power of neem leaf biological pesticides against anthracnose on dragon fruit plants in vitro. The concentration treatments of biological pesticides included 0%, 20%, 40%, and 60%. The results obtained showed that the biological pesticide neem leaf with a concentration of 20% already had an inhibitory power against the pathogen Colletotrichum sp. which is quite good because at that concentration it already has a fairly high antifungal compound. The magnitude of the inhibitory power of neem leaf biological pesticides at a concentration of 20% was 53.94%.
Promotion of Beauveria bassiana Mushroom on Different Growing Media and Its Pathogenicity on Insects Lukmanul Hakim; Yenny Wuryandari
Nusantara Science and Technology Proceedings Seminar Nasional Agroteknologi 2022
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2023.3116

Abstract

One type of entomopathogenic fungus that is known to be effective in controlling important plant pests is the Beauveria bassiana fungus. The fungus B. bassiana, has a high reproductive capacity, is easy to produce and under unfavorable conditions can form spores that can last a long time in nature. The purpose of this study was to determine the density and viability of B. bassiana spores on corn and rice media and to investigate the process of B. bassiana fungal infection in the test insects. The method used is; Fungal propagation test on Rice and Corn Media, Microscopic Observation of Fungus from Corn and Rice Media, Calculation of Fungus Spore Density, Calculation of Fungus Viability and Pathogenicity Test. The research results obtained are as follows; Results The propagation of B. bassiana mushroom grew on the seventh day, the fungus growth was faster in rice media than in corn media. The spore density of the fungus B. bassiana clove bondowoso in corn media was 3.75x108 spores/ml, rice media was 4.58x108 spores/ml, B. bassiana banyuwangi coffee medium was 3.5x108 spores/ml, rice media was 3.75x108 spores/ml. The viability of the fungus B. bassiana, clove bondowoso and banyuwangi coffee on rice and corn media was obtained on average above 60%. The results of the pathogenicity test of B. bassiana on insects began to appear on the tenth day after the baiting process.
Co-Authors Lukmanul Hakim Mochammad Mirza Saputra Nolla Dwi Elvira Noni Ramadhini