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THE APPLICATION OF Trichoderma viride STRAIN FRP 3 FOR BIODEGRADATION OF GLYPHOSATE HERBICIDE IN CONTAMINATED LAND Arfarita, Novi; Djuhari, Djuhari; Prasetya, Budi; Imai, Tsuyoshi
AGRIVITA, Journal of Agricultural Science Vol 38, No 3 (2016): OCTOBER
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v38i3.550

In this current study, we observed Trichoderma viride strain FRP3 capability for biodegradation of glyphosate on contaminated land in Indonesia. There were two blank plots that have been involved as representatives of indigenous fungal, that prepared as control (non-contaminated soil) and P1 (GP-contaminated soil) while the treatments were represented by two plots. Plot 2 (P2) was introduced with conidia suspension of Trichoderma viride strain FRP3 one time application, and plot 3 (P3) was introduced with conidia suspension of Trichoderma viride FRP3 two time applications. At the end of observation, the CFU of two times application was the highest with CFU of 15.97 x 106 gr-1 soil. The CFU of P3 was corresponding to 45% higher than P2 (8.83 x 106 gr-1 soil). The CFU of GP-contaminated soil without conidia suspension application had 0.66 x 106 gr-1soils, only 0.7% and 0.4% corresponding to P2 and P3, respectively. Direct indicator of glyphosate degradation was determined using GC analysis. Within 7 days after Trichoderma viride FRP3 was introduced, glyphosate content of treated soil decreased. This fungal strain provided 48% (P2) and 70% (P3) of glyphosate degradation higher than indigenous soil microbial community (P1) within 28 days of application.

Interaction between Ground Water and Surface Water in Porong River, Sidoarjo Haromain, Safira Anisah; Yuliani, Emma; Andawayanti, Ussy; Imai, Tsuyoshi; Aliyah, Firda Samaniyatul
Jurnal Teknik Pengairan: Journal of Water Resources Engineering Vol. 15 No. 1 (2024)
Publisher : Fakultas Teknik, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.pengairan.2024.015.01.3

The Lapindo mud phenomenon has been going on for 17 years, causing damage to the land and subsurface geological structures which raises concerns about reducing groundwater quality. This research aims to determine groundwater flow patterns based on surface contours around the Porong River which were analyzed using Surfer 13 software, and understand the relationship between groundwater and surface water through physical properties with correlation coefficients using Rstudio, as well as to identify the chemical characteristics of surface water and groundwater using the Trilinear Piper Diagram. Based on the research results, the groundwater flow pattern moves from northeast to southwest, and there is interaction between the two as indicated by the physical properties of the water. The correlation coefficient value ranges from 0.615 to 0.997, meaning that the relationship between groundwater and surface water is moderate to strong. Both groundwater and surface water have the same chemical type, namely mostly containing alkaline earth and weak acids. In addition, this research reveals the exploration of the impact of heavy metal parameters, namely Copper (Cu), from the Lapindo mud in the Porong River and residents' dug wells. In conclusion, this research shows the interaction between groundwater and surface water in the Porong River.

Interaction between Ground Water and Surface Water in Porong River, Sidoarjo Haromain, Safira Anisah; Yuliani, Emma; Andawayanti, Ussy; Imai, Tsuyoshi; Aliyah, Firda Samaniyatul
Jurnal Teknik Pengairan: Journal of Water Resources Engineering Vol. 15 No. 1 (2024)
Publisher : Fakultas Teknik, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.pengairan.2024.015.01.3

The Lapindo mud phenomenon has been going on for 17 years, causing damage to the land and subsurface geological structures which raises concerns about reducing groundwater quality. This research aims to determine groundwater flow patterns based on surface contours around the Porong River which were analyzed using Surfer 13 software, and understand the relationship between groundwater and surface water through physical properties with correlation coefficients using Rstudio, as well as to identify the chemical characteristics of surface water and groundwater using the Trilinear Piper Diagram. Based on the research results, the groundwater flow pattern moves from northeast to southwest, and there is interaction between the two as indicated by the physical properties of the water. The correlation coefficient value ranges from 0.615 to 0.997, meaning that the relationship between groundwater and surface water is moderate to strong. Both groundwater and surface water have the same chemical type, namely mostly containing alkaline earth and weak acids. In addition, this research reveals the exploration of the impact of heavy metal parameters, namely Copper (Cu), from the Lapindo mud in the Porong River and residents' dug wells. In conclusion, this research shows the interaction between groundwater and surface water in the Porong River.

Exploration of indigenous free nitrogen-fixing bacteria from rhizosphere of Vigna radiata for agricultural land treatment Arfarita, Novi; Muhibuddin, Anton; Imai, Tsuyoshi
Journal of Degraded and Mining Lands Management Vol. 6 No. 2 (2019)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2019.062.1617

Nitrogen is the most abundant element in the atmosphere, however, most often deficient in agricultural lands. This research was an exploratory to get indigenous non-symbiotic nitrogen-fixing bacteria. Soil samples were collectedfrom rhizosphere of green beans. This study was aimed to determine the bacterial population of the three regions; screening, isolation and selection of free nitrogen-fixing bacteria. Antagonism and pathogenicity tests were performed to observe its potential for a biofertilizer product. The highest number of free nitrogen-fixing bacteria was found from forest soil sample of 2.5x 1011CFU/ml. Screening and isolation process has obtained 10 free nitrogen-fixing isolates. Then was selected into 4 isolates namely SNF4, SNF5, SNF7 and SNF8 according to the ammonia production test qualitatively. When an antagonism activity performed, there was no inhibition zone each other. The pathogenicity test did not show the pathogenic symptom. This study also showed that bacterial isolates obtained significantly affected the germination growth of green beans compared to controls. Possibility, bacteria of this type produced growth hormone for a plant. Strain SNF8 has shown the highest ammonium production then was selected for 16S rRNA identification. Similarity test of genome sequence of strain SNF8 had 99% similarity with Bacillus cereus.

Enhancing bare land soil quality using electric induction apparatus in combination with rabbit urine liquid fertilizer application to support garlic (Allium sativum) production Arfarita, Novi; Afroni, Mohammad Jasa; Sugiarto, S; Imai, Tsuyoshi
Journal of Degraded and Mining Lands Management Vol. 7 No. 4 (2020)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2020.074.2381

The new approach in this study was using electric induction technique to enhance soil fertility of bare land in a combination of rabbit urine application in the form of liquid fertilizer. This approach had been exaggerating soil microbial activities and modifying the exchange capacity of anions and cations in soil. This study aimed to find out the best combination of different duration of electricity induction and rabbit urine liquid fertilizer at different levels of their application. Randomized factorial block design with two factors was used for this study. The first factor was the duration of the electric induction at the rates of 0, 30, 45, and 60 minutes, while the application of rabbit urine as liquid organic fertilizer was at the rates of 0, 100, 150, and 200 mL/L. Plant height, number of leaves and stem diameter, fresh and dry weight of garlic bulb were measured within 3 months. The observations were carried out using destructive and non-destructively approached with intervals of 7 days. The best treatment that gave the highest plant height, number of leaves, stem diameter, tuber diameter, fresh and dry garlic bulb and garlic production was found under the combination of 60 minute of electricity induction and 200 mL/L of urine rabbit application (P<0.05). Also, this treatment was successfully to give garlic production of 8.56 t/ha.

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