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Wijanarka Wijanarka

Fakultas Sains dan Matematika, Universitas Diponegoro, Semarang

Author-ID : 5961139

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Materials Science & Nanotechnology

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Uji bakteriologis air di PDAM GT Kabupaten Magelang dengan metode MPN Quanty-Tray Lailatul Mubarokhah; Wijanarka Wijanarka
Jurnal Biologi Papua Vol 11, No 1 (2019)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

Water is the main need for life. The bacteriological requirements for clean water are the presence or absence of Coliform bacteria and E. coli in water. The purpose of this study was to determine the quality of water from gravity springs and pump springs as a source of water in Magelang Regency PDAM GT and Magelang Regency community. This study was to finding out the bacteriological test of water in Magelang Regency PGT. This study was to know bacteriological water testing techniques using the MPN Quanti-method Tray in Magelang Regency PGT. The method used in this study is the Quanty-Tray Most Probable Number (MPN) method. The sample of this study were two gravitational springs namely Sample A and Sample B and two pumping springs namely Sample C and Sample D. The results of MPN calculations from the four springs did not meet the requirements of clean water ready to be distributed to the community because Coliform in the spring gravity water and pumps and found E. coli in the pump spring water. Key words: coliform, MPN quanty-tray, bacteriological requirement.

Aktivitas Enzim Inulinase dan Laju Pertumbuhan Spesifik Isolat Bakteri IS-1 pada Medium Tepung Umbi Dahlia Wijanarka Wijanarka; Maulida Aqlia; Kristina Kristina
Jurnal Biologi Papua Vol 10, No 2 (2018)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

Dahlia bulbs (Dahlia variabilis) contains carbohydrates in the form of inulin. Inulin is a form of fructose polymer from fructose monomers. Inulin hydrolysis produces a product in the form of fructose.Inulinase enzyme is an enzyme able to purely hydrolyze inulin. This study aims to determine the activity of inulinase enzymes and the specific growth rate of IS_1 bacterial isolates producing inulinase in medium of Dahlia tuber flour. Isolates of inulinase-producing bacteria were isolated directly from decay dahlia tubers and used as an inoculum in creating starter.The measurement of cell growth was carried out by inoculating a 20-hour starter on the production medium. Incubation is done for 24 hours using a rotary shaker with a speed of 120 rpm. Sampling was carried out every 4 hours at 0-hour (T0), 4 hours (T4), 8 hours (T8), 12 hours (T12), 16 hours (T16), 20 hours (T20) and 24 hours (T24) intervals with a sample of 5 mL measured for absorbance of λ 520 nm using spectrophotometer. Production medium culture in each sample was taken 1 mL and centrifuged at 3000 rpm for 10 minutes to obtain supernatant of crude enzyme. Measurement of inulinase enzyme activity was carried out using 3,5-dinitrosalicylic acid (DNS) method and spectrophotometric method at λ 570 nm. IS_1 bacterial isolate showed optimum growth at incubation time of 8 hours with OD of 0.647 and had a specific growth rate (µ) of 0.02 / hour with a generation time of 34.65 hours. The highest enzyme activity produced by IS_1 bacteria at 8 hours incubation time was 0.490 IU. The conclusion of this study is that IS_1 bacteria indicates good growth in medium of dahlia tuber flour and has inulinase enzyme activity which is able to hydrolyze inulin into fructose. Key words: inulin, bacterial isolate, inulinase, production

Identifikasi Molekular Bakteri Glukanolitik Indigenous KE-B6 dari Saluran Pencernaan Bekicot (Achatina fulica) Wijanarka Wijanarka; Sri Pujiyanto; Budi Raharjo
Jurnal Biologi Papua Vol 11, No 2 (2019)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

Glucanolitic bacteria are bacteria that have the ability to break down glucan into glucose monomer units. The ability of the bacteria is caused by the presence of glucanase enzymes. The choice of glucanase derived from bacteria is based on the ability and speed of bacterial growth in terms of producing glucanase enzymes. The presence of bacteria and protozoa in the digestive tract symbiotic with each other to digest cellulose or concomitant materials . Based on the ability of the way of life to digest forage and leaf litter, it is suspected that snails (Achatina fulica) have the ability to produce glucanase biocatalysts, especially in the digestive tract. To find out the characteristics and characteristics of indigenous bacteria snail canals, identification of KE-B6 bacteria is carried out molecularly so that accurate and accurate results are obtained. The Basic Alignment Search Tools BLAST results of KE-B6 bacterial isolates based on 16S rDNA sequence data with 27F (Forward) and 1492R (Reverse) primers showed that these bacterial isolates had homology of 99.64% to Serratia marcescens. Key words: bacteria, glucanolitic, A. fulica, Serratia marcescens.

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