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Lemongrass Powder in Bio-Bs Effervescent Formulation of Lombok Island Local Isolate on Viability and Amount of Bacillus Sphaericus Toxin Crystal Protein for Control of Anopheles sp. Larvae Yunan Jiwintarum; Zainal Fikri; Maruni Wiwin Diarti; Rabi'unnisa Sulaimah
Jurnal Analis Medika Biosains (JAMBS) Vol 10, No 1 (2023): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v10i1.266

Abstract

Bio Formula – BS effervescent (Bio- Bacillus sphaericus) is an Effervescent powder-shaped formula that is easy to use by the public. Lombok's local effervescent bio-BS isolated formula has a weakness in terms of smell. The results from the literature search that fragrant lemongrass has a distinctive smell and can kill Aedes aeygpti, but scientific data has not been obtained about the ability of fragrant lemongrass to kill Anopheles Sp in the form of larvae and mosquitoes. The study aimed to find out the effect of the combination formulation of Bio-BS Effervescent local isolate Lombok Island with the addition of lemongrass powder for viability and the amount of toxin crystal protein Bacillus sphaericus. This research method is exploratory and experimental in the laboratory with the design of Post Test Only Control Group Design. The study used six treatment formulations. Samples of Anopheles sp larvae research in Batu Layar lagoon, West Lombok regency and pelur lagoon in Peringgesela, East Lombok regency and from the results of colonization of larvae. The independent Variable is a combination formulation of Bio-BS Effervescent isolated locally from Lombok Island with the addition of fragrant lemongrass powder. The dependent variable is the mortality of Larvae Anophels Sp, Viability of B. Sphaericus, and Amount of Toxin Protein Production of B. Sphaericus. The larvae death rate, the concentration of cells/endospores, and the number of repeats in each container are then tabulated and analyzed using Probit Analysis with the help of MINITAB 16 software. B. sphaericus viability data and the amount of endospore toxin protein crystal production were descriptively analyzed.
EFEKTIVITAS RENDAMAN DAUN RAMBUTAN DALAM PENGENDALIAN VEKTOR MALARIA SETELAH GEMPA Erlin Yustin Tatontos; I Wayan Getas; Maruni Wiwin Diarti; Urip
Jurnal Penelitian Pendidikan IPA Vol 10 No 11 (2024): November
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i11.8639

Abstract

After the earthquake that occurred on the island of Lombok during August 2018 malaria appeared again. The purpose of this study was to determine malaria infection and control of Anopheles sp with Rambutan leaves. The research method uses analytical observation for malaria infection and an experimental with completely randomized design for Anopheles sp. control using natural ingredients, namely rambutan leaves . The population and sample were suspected malaria and Anopheles sp larvae in the working area of ​​the Penimbung Public Health Center in West Lombok District affected by the earthquake. Results of malaria examination studies with RDT/malaria sticks 13% positive for malaria, whereas microscopically 25% positive for malaria. Laboratory test results with rambutan leaf soaking concentrations of 1%, 3%, 6%, 9% and 12% were obtained the higher the concentration the more effective the killing power against larvae of Anopheles sp, with LC50 12.353% and LC90 19.666%. Field test results no larvae in the cupak with the soaking of rambutan leaves. Conclusion, 25% of the population affected by the earthquake were infected with malaria, the most common being plasmodium falciparum, soaking rambutan leaves is effective for controlling Malaria vectors in laboratorium and fields.