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All Journal Jurnal Sain Peternakan Indonesia Bulletin of Applied Animal Research
Satyaguna Rakhmatulloh
Departemen Teknologi Hasil Ternak, Fakultas Peternakan, Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary

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Penggunaan Ekstrak Tomat untuk Peremajaan L. acidophilus FNCC sebagai Media Alternatif pada Penyiapan Starter Fermentasi Ari Surya Sukarno; Widodo; Satyaguna Rakhmatulloh; Nurliyani; Endang Wahyuni
Bulletin of Applied Animal Research Vol 5 No 1 (2023): Bulletin of Applied Animal Research
Publisher : LPPM Perjuangan University of Tasikmalaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36423/baar.v5i1.1222

Abstract

Lactic acid bacteria are a milk fermentation starter that relies on MRS media as a source of nutrition for culture refreshment. This study aims to determine the growth and viability of L. acidophilus FNCC on tomato extract media as an alternative medium. Tomato extract was obtained by sterilization at 121°C with a pressure of 15 Psi for 15 minutes, and the liquid from the tomatoes was collected. L. acidophilus FNCC was grown in tomato extract medium and liquid MRS as a control for 12 hours at 37°C. The amount of biomass growth was measured at 0, 2, 6, 8, 10, and 12 hours based on the increase in turbidity using an absorbance of λ620. Storage viability was observed for changes in the total number of lactic acid bacteria every week for 28 days. The analysis was repeated three times, and data analysis was carried out using a Randomized Design One Way Annova. Incubation L. acidophilus FNCC for 12 hours resulted in an absorbance value of 1.44 ± 0.02 on MRS media which was higher (P<0.01) than using tomato extract, which only reached 0.04 ± 0.02. The storage viability of L. acidophillus FNCC for 28 days in tomato extract media was maintained with the same amount of log log 8.85 ± 0.58 cfu/mL cfu/mL (P>0.05) with the MRS control medium. Therefore, tomato extract can be used as an alternative medium to grow and store L. acidophilus FNCC for 28 days.
Antibacterial and Antioxidant Activity of Protein Hydrolysate Extracted from different Indonesian Avian Egg White A. S. Sukarno; Nurliyani Nurliyani; Yuny Erwanto; Satyaguna Rakhmatulloh; Rifqi Rifqi
Jurnal Sain Peternakan Indonesia Vol 18 No 1 (2023)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jspi.id.18.1.27-33

Abstract

White egg contains the most significant protein source that has been explored to give more benefit for health purposes.  This study evaluated the potential of antibacterial and antioxidant activity of white egg protein hydrolysate extracted from Indonesian avian eggs.  The avian egg materials used local Indonesian chicken eggs (kampung chicken), chicken breed eggs, duck eggs, and muscovy duck eggs.  The white egg was extracted by organic solvent and produced protein extracted which was dominated by ovalbumin and ovotransferrin.  The protein extracted was hydrolyzed by pepsin and then powdered using a freeze dryer.  Antibacterial and antioxidant activity was evaluated by agar well diffusion technique and 2,2-Diphenyl-1-picryl hydroxyl (DPPH) radical scavenging method.  All samples showed antimicrobial activity against Salmonella sp., Escherichia coli, Staphylococcus aureus, Bacillus cereus, and Bacillus subtilis with a concentration of protein hydrolysate at 40 mg/mL.  The antioxidant activity between avian species was different and dose-dependent.  Protein hydrolysate extracted from duck white egg exhibited the highest radical scavenging activity (65%) at 10 mg/mL concentration.  However, all white egg protein hydrolysate extracted from Indonesian avian eggs can potentially help reduce oxidation and microbial deterioration.
Co-Authors A. S. Sukarno Ari Surya Sukarno Endang Wahyuni Nurliyani Nurliyani Nurliyani Rifqi Rifqi Widodo Yuny Erwanto