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Ni Luh Gede Ratna Juliasih
Jurusan Kimia, Universitas Lampung
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Environmental Science

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Isolasi, Karakterisasi, dan Identifikasi Bakteri Endofit Sargassum polycystum Penghasil Enzim Alginat Lyase Yesica Bella Safitri; Agus Setiawan; Ni Luh Gede Ratna Juliasih; Endang Linirin Widiasuti; Gregorius Nugroho Susanto
Journal of Tropical Marine Science Vol 6 No 1 (2023): Journal of Tropical Marine Science (on going)
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/jour.trop.mar.sci.v6i1.3732

Abstract

Alginate, polysaccharide from marine brown algae, is widely used for antitumor, anti-inflammatory, and also antiviral. However, alginate has large molecular weight that difficult to digest in some organsims. Therefore, it is important to look for the alginate lyase enzyme to break down the alginate. The purpose of this study was to obtain bacterial isolates that produce alginate lyase enzymes from Sargassum polycystum. Isolation of alginate lyase-producing bacteria was carried out by collecting Sargassum polycystum. from Sebalang Beach, Tarahan, South Lampung then the bacteria were isolated by the dilution method. The colonies obtained were purified and selected using qualitative (clear zone) and semi-quantitative activity tests, and identified morphologically, biochemically and molecularly using the 16S-rDNA gene. In this study, 20 pure bacterial isolates were obtained with one isolate having alginate lyase activity, namely PTF and PTH. The results of biochemical tests and molecular characterization showed that the isolate was Bacillus cereus.
Efektivitas Perlindungan Udang Vaname (Litopenaeus vannamei) terhadap Infeksi White Spot Syndrome Virus (WSSV) dengan Suplementasi Natrium Alginat Sargassum sp. Dari Perairan Lampung dan Kombinasi dengan Vitamin C Muhammad Darmawan; Agus Setiawan; Ni Luh Gede Ratna Juliasih; Hilma Putri Fidyandini
Journal of Tropical Marine Science Vol 6 No 1 (2023): Journal of Tropical Marine Science (on going)
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/jour.trop.mar.sci.v6i1.3819

Abstract

White spot syndrome virus (WSSV) is a viral infectious disease that can cause high mortality in Pacific white shrimp culture. The shrimp (±19 g) were reared in 9 containers with a density each of 10 shrimp/container and fed with shrimp feed at 3% of biomass daily. Shrimp were grouped into three treatments each with triplicate, namely feeding without alginate supplementation as a control, supplementation with sodium alginate supplementation of Sargassum sp. with a dose of 1 g/kg of feed + 0.2 g/kg feed of vitamin C, and supplementation of sodium alginate Sargassum sp. at a dose of 2 g/kg of feed. After 14 days of treatment, shrimp were injected with white spot syndrome virus filtrat in phosphate buffer saline (PBS) (1:1 v/v) for challenge assay. Several parameters were observed included survival rate (SR), relative percent survival (RPS), mean time to death (MTD), shrimp behaviour and clinical symptoms, hepatopancreatic histology, confirmation of white spot syndrome virus infection by PCR, and water quality. The results showed that supplementation of sodium alginate Sargassum sp. protected shrimp against white spot syndrome virus 14.80 hours longer in survival compared to the control group. However the supplementation of sodium alginate Sargassum sp. from Lampung waters not yet to protect shrimp against white spot syndrome virus after 72 hours infection.
Co-Authors Agus Setiawan Endang Linirin Widiasuti Gregorius Nugroho Susanto Hilma Putri Fidyandini Muhammad Darmawan Yesica Bella Safitri