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All Journal Indonesian Journal of Biotechnology Jurnal Pengabdian Magister Pendidikan IPA
Yohanes Harvinda
Fisheries Science Postgraduate Program, Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada, Jalan Flora A4, Bulaksumur, Yogyakarta 55281, Indonesia
Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Materials Science & Nanotechnology

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Production, purification and characterization of chitinase from Micromonospora sp. AR17 Yohanes Harvinda; Ustadi Ustadi; Masagus Muhammad Prima Putra
Indonesian Journal of Biotechnology Vol 28, No 1 (2023)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.77137

Abstract

N‐acetylglucosamine (NAG) is the monomer product of chitin, which has been widely used as a bioactive com‐ pound in applications such as anti‐tumor, anti‐microbial, and antioxidant activities. In production, biological processes using enzymes are preferable to chemicals due to environmental issues. This study aims to determine the activity, purity level, and molecular weight of purified chitinase from Micromonospora sp. AR17 determines the concentration of NAG produced by purified chitinase that has been characterized. Chitinase was produced by fermentation in colloidal chitin broth at 40 °C, pH 7, for 7 days, while chitinase activity was checked every 24 h. The optimal fermentation time was used to produce chitinase for a further purification step. Enzyme purification was carried out by ultrafiltration, ammonium sulfate precipitation, ion exchange chromatography (Q Sepharose Fast Flow), and gel filtration (Sephacryl S‐300). The purified enzyme was then char‐ acterized for optimum time, pH, and temperature to produce NAG. The results suggested that the fourth day was the optimal time for chitinase production, with chitinase activity of 0.0040 U/mL and a NAG concentration of 7.62 µg/mL. The purifica‐ tion step successfully increased the purity by 6.82 times with chitinase‐specific activity at 1.4648 U/mg. Production of NAG with purified chitinase produced a NAG concentration of 32.472 µg/mL with an incubation time of 30 min at 40 °C and pH 7.
Implementasi Inovasi Probiotik Berbasis Limbah Pasar untuk Meningkatkan Produktivitas Ikan Nila di Kelompok Krido Yuwono Mina Padi Technopark, Panembangan, Banyumas Sujadi, Frentina Murti; Anjaini, Jefri; Simangunsong, Tohap; Setiyaningsih, Lilik; Mardika, Hanisya Putri Kania; Setyawan, Agung Cahyo; Sukardi, Purnama; Kusuma, Baruna; Nurhabib, Asro; Marnani, Sri; Harvinda, Yohanes
Jurnal Pengabdian Magister Pendidikan IPA Vol 8 No 4 (2025): Oktober-Desember 2025
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpmpi.v8i4.13355

Abstract

The probiotic innovation training programme based on market waste implemented at the Krido Yuwono Mina Padi Technopark Group aims to increase tilapia productivity and farming efficiency through the use of organic waste as raw material for probiotics. This training involves participants in the production of probiotics through the fermentation of market waste using lactic acid bacteria starters. The evaluation results show an increase in tilapia productivity of around 15-20% with better feed efficiency and a reduction in ammonia levels in pond water, which was previously a major problem in maintaining fish health. The community involved in this programme also increased their confidence in managing tilapia farming efficiently and received new knowledge that can reduce operational costs. This programme has achieved significant social and economic impacts and is expected to develop into a sustainable community empowerment model in the field of aquaculture and support more environmentally friendly waste management.
Co-Authors Frentina Murti Sujadi Kusuma, Baruna Lilik Setiyaningsih Mardika, Hanisya Putri Kania Masagus Muhammad Prima Putra Nurhabib, Asro Purnama Sukardi Setyawan, Agung Cahyo Sri Marnani Tohap Simangunsong Ustadi Ustadi