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All Journal Health Sciences and Pharmacy Journal
Christivanny Sheila Pravita
Sekolah Tinggi Ilmu Kesehatan Nasional
Health Professions Medicine & Pharmacology Nursing Public Health

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Penetapan kadar flavonoid total perasan lemon (Citrus limon (L.) Osbeck) secara spektrofotometri UV-Vis Christivanny Sheila Pravita; Crescentiana Emy Dhurhania
Health Sciences and Pharmacy Journal Vol 7, No 1 (2023)
Publisher : STIKes Surya Global Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32504/hspj.v7i1.653

Abstract

One of the fruits whose juice can function as an antibacterial is a lemon. This is related to the flavonoid content in lemons. The purpose of this study was to determine the total flavonoid content in local and imported lemon (Citrus limon (L.) Osbeck) juice using the UV-Vis spectrophotometric method. The filtered juice was used for qualitative analysis and quantitative analysis. Qualitative analysis using concentrated HCI reagent plus magnesium powder, 10% NaOH, and concentrated H2SO4 showed that lemon juice was positive for flavonoids. Quantitative analysis was performed using UV-Vis spectrophotometry with an operating time of 28 minutes, the maximum wavelength of 439 nm. The total flavonoid content in local lemon juice was 38.42 ± 0.448 mg QE/100 ml of juice, with a coefficient of variation obtained was 1.165%. The total flavonoid content in imported lemon juice was 39.76 ± 0.199 mg QE/100 ml of juice with a coefficient of variation was 0.501%.
Co-Authors Crescentiana Emy Dhurhania