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All Journal JURNAL KIMIA SAINS DAN APLIKASI
Bustanussalam Bustanussalam
Biotechnology Research Center, Indonesian Institute of Sciences Bogor
Chemical Engineering, Chemistry & Bioengineering Chemistry Engineering

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The Combination of Carbon Source and the Addition of Phenylacetic Acid (PAA) to Growth Medium Penicillium chrysogenum to Enhance of Penicillin (Pen G) Production Martha Sari; Dirayanti Dirayanti; Eris Septiana; Bustanussalam Bustanussalam; Apon Zaenal Mustopa
Jurnal Kimia Sains dan Aplikasi Vol 23, No 9 (2020): Volume 23 Issue 9 Year 2020
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (48.721 KB) | DOI: 10.14710/jksa.23.9.312-318

Abstract

The nutrition factor governs the growth and production of Penicillin G (Pen G) by Penicillium chrysogenum in a broth medium. Proper nutrition can improve Pen G antibiotic production. In this research, the optimal condition for Pen G production from P. chrysogenum in a standard culture medium and various carbon sources medium (glucose, lactose, maltose, and sucrose) were done for ten days. Phenylacetic Acid (PAA) precursor at 0.0 – 0.6 gL-1 (increment 0.1) was used to improve Pen G production. The Pen G was detected by HPLC, compared with the standard (Penicillin G Sodium Salt). The results showed that the PDB standard medium and lactose medium (150 rpm, at 30°C) produced 0.425 gL-1 and 0.107 gL-1 Pen G. Addition of 0.6 gL-1 PAA improved the Pen G production up to 0.045 gL-1 in the PDB medium, become the final concentration of 0.470 gL-1 and 2.460 gL-1 in the lactose medium, become the final concentration of 2.565 gL-1. The antibiotic’s activity against the pathogenic bacteria, i.e., B. subtilis, S. aureus, and S. typhi employing the disk diffusion method, has been done. The TLC method’s detection of the potential Pen G spots was conducted with ethyl acetate: distilled water: acetic acid (60:20:20) as the mobile phase. The Pen G extracts could inhibit the growth of all tested bacteria in Rf 0.65. This study informs the proper combination of carbon source and precursor effects and increases the bioproduction of Pen G from P. chrysogenum.
The potential of Endophytic Fungal Extract Isolated from Cinnamon (Cinnamomum burmannii) as Antidiabetic and Antioxidant Eris Septiana; Fauzy Rachman; Yatri Hapsari; Yadi Yadi; Bustanussalam Bustanussalam; Siti Irma Rahmawati; Fauzia Nurul izzati; Partomuan Simanjuntak
Jurnal Kimia Sains dan Aplikasi Vol 22, No 6 (2019): Volume 22 Issue 6 Year 2019
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3558.055 KB) | DOI: 10.14710/jksa.22.6.275-282

Abstract

An increase in blood glucose levels in people with diabetes can cause an increase in free radicals, which can worsen the disease. Thus, drugs that have antidiabetic and antioxidant activities are needed. The cinnamon plant is high in antioxidants and has long been used as a source for a diabetes drug. The utilization of endophytic fungi isolated from cinnamon plants as antidiabetic and antioxidant has never been reported. This study aims to investigate the antidiabetic as well as antioxidant activity from the extract of endophytic fungi from the cinnamon plant. The antidiabetic activity was tested using the α-glucosidase enzyme inhibition method, while antioxidant activity was tested using the DPPH free radical scavenging method. Total phenol content was measured based on the Follin-Ciocalteu reagent reaction. All endophytic fungal extracts from the cinnamon leaves, twigs, flowers, and fruit have antidiabetic and antioxidant activity as well as high total phenol content. The three parameters measured showed a positive correlation. Endophytic fungal extract of Cb.D6 isolate derived from the leaf had the highest antidiabetic and antioxidant activity among the other isolates amounting to 92.41% and 90.28%, respectively. In addition, the total phenol content of Cb.D6 isolates was also the highest with 357.83 mg equivalent to gallic acid/g extract. Therefore, the endophytic fungal extract of Cb.D6 isolate has the potential to be developed as a source of the antidiabetic and antioxidant ingredients.
Co-Authors Apon Zaenal Mustopa Dirayanti Dirayanti Eris Septiana Fauzia Nurul izzati Fauzy Rachman Martha Sari Partomuan Simanjuntak Rahmawati, Siti Irma Yadi Yadi Yatri Hapsari