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All Journal ALCHEMY Jurnal Penelitian Kimia
Noerma Juli Azhari
Jurusan Kimia, FMIPA, Universitas Brawijaya, Jl. Veteran, Malang 65145 telp. (0341) 575838
Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Environmental Science Materials Science & Nanotechnology

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The Development of Conductometric Biosensor for Determination of Uric Acid Concentration in Human Serum Using Screen Printed Carbon Electrode (SPCE) – Nata De Coco Abdi Naufal Ramadhan; Luluil Maknun; Noerma Juli Azhari
ALCHEMY Jurnal Penelitian Kimia Vol 11, No 2 (2015): September
Publisher : UNIVERSITAS SEBELAS MARET (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/alchemy.11.2.724.192-199

Abstract

The control of uric acid is important. A high level of uric acid can cause gout disease. Therefore a simple, fast, and accurate method for uric acid determination is required. In this research a conductometric biosensor has been developed by SPCE – Nata de coco for uric acid determination. The prepared biosensor was optimized to get a good performance of biosensor and it is applicable for human serum samples. The optimized variables were enzyme concentration, membrane thickness and pH solution. The various enzyme concentration were 6 μg/mL; 12 μg/mL; 18μg/mL; 24 μg/mL. The various membrane thickness were 5 μm; 10 μm; 15 μm. Meanwhile, the various pH solution were 7; 7.5; 8; 8.5; 9. The optimum enzime concentration was 18 µg/mL with the membrane thickness and pH were 5 µm and 8, respectively. The prepared biosensor can determine the uric acid concentration at range of 0 – 1.2 ppm with the sensitivity of 7.74 µS/ppm and the limit detection is 0 ppm. The biosensor was applied to uric acid detection in human serum with accuracy of 95 %.
Co-Authors Abdi Naufal Ramadhan Luluil Maknun