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Wa Ode Harlis
Department of Biology, Faculty of Mathematics and Natural Sciences, University of Halu Oleo
Biochemistry, Genetics & Molecular Biology Chemistry Mathematics Physics

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Effectiveness of snail mucus gel (Achatina fulica Ferr) on mice (Mus musculus L.) burns Wa Ode Harlis; Dwi Arinto Adi; Ni Made Maitri Saraswati; Jamili Jamili; Suriana Suriana; Resman Resman
Jurnal Pijar Mipa Vol. 18 No. 3 (2023): May 2023
Publisher : Department of Mathematics and Science Education, Faculty of Teacher Training and Education, University of Mataram. Jurnal Pijar MIPA colaborates with Perkumpulan Pendidik IPA Indonesia Wilayah Nusa Tenggara Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpm.v18i3.4803

Abstract

This study aimed to determine the effectiveness of snail mucus gel (Achatina fulica) on the length of time for burns healing, burns diameter, and the percentage of burns healing in mice (Mus musculus). The type of this research was experimental with a completely randomized design (CRD) method with 20 male mice divided into five treatment groups, namely NC (Negative Control; gel basis), PC (Positive Control; Bioplacenton 10%), P1 (3% gel), P2 (4% gel) and P3 (5% gel), with four replications for each treatment. The making of burns on the back of mice was done by using metal coins that were heated to become coals with an initial diameter of 20 mm, which are affixed for 1 second. The parameters observed were the length of time for burns healing, burns diameter, and the percentage of burns healing for 16 days. The data were analyzed by using Way ANOVA (Analysis Of Variance) (α = 0.05%) and continued with the LSD (Least Significant Different) test. The results showed that snail mucus gel (A. fulica) with a gel concentration of 5% was more effective in accelerating burns healing with an average healing time of 14 days, and positive controls healed within 14. In 25 days, 4% gel recovered in 14.5 days, 3% in 15 days, and negative controls recovered in 16 days. The fastest closure of burns diameter was in gel with a concentration of 5% with a mean of burns diameter of 0.33 mm and an average percentage of burn healing of 98.35%, followed by the Bioplacenton treatment group of 10%, gel with a concentration of 4%, 3%, and the longest burns closure was in the ointment base gel group.
Anti-infertility Effects of Zinc (Zn) Supplements on the Spermatozoa Quality of Rats (Rattus norvegicus L.) Exposed to Cigarette Smoke Wa Ode Harlis; Rasna Rostika Rasna; Adi Karya; Resman Resman
Jurnal Pijar Mipa Vol. 21 No. 1 (2026)
Publisher : Department of Mathematics and Science Education, Faculty of Teacher Training and Education, University of Mataram. Jurnal Pijar MIPA colaborates with Perkumpulan Pendidik IPA Indonesia Wilayah Nusa Tenggara Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpm.v21i1.11165

Abstract

Cigarette smoke contains nicotine compounds (C10H14N2), tar and carbon monoxide (CO), which cause inhibition of Leydig cells, damage to seminiferous tubules and damage to spermatozoa cells. The aim of this study was to determine the anti-infertility effect of Zinc supplements on the quality of Wistar rat (Rattus norvegicus L.) spermatozoa exposed to cigarette smoke. This research is an experimental study consisting of 4 treatments with 3 replications: Control (drinking water), P1 (14 mg/g BW), P2 (28 mg/g BW), and P3 (42 mg/g BW) Zinc. Exposure to cigarette smoke was carried out twice a day for 14 days in all treatments, and zinc was given for 14 days in treatments P1, P2 and P3. On the 29th day, mice were sacrificed by anesthesia using chloroform and dissected to observe the quality of spermatozoa with parameters of motility, viability and morphology. Data were analyzed using ANOVA (Analysis of Varience) and the BNT test with a 95% confidence level. The results showed that Zinc supplementation in treatments P1 and P2 significantly improved spermatozoa quality, whereas in P3 it decreased significantly. The conclusion of this study is that Zinc (Zn) supplements at a dose of 14 mg/g BW (P1) and a dose of 28 mg/g BW (P2) have an anti-infertility effect by improving the quality of rat spermatozoa that have been exposed to cigarette smoke. The results of this research can be important information for improving male reproduction due to exposure to free radicals from cigarette smoke.
Co-Authors Adi Karya Dwi Arinto Adi Jamili Jamili Ni Made Maitri Saraswati Rasna Rostika Rasna Resman Resman Suriana Suriana