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All Journal Journal of the Indonesian Medical Association : Majalah Kedokteran Indonesia Berita Kedokteran Masyarakat
Enny Suswati
Department of Microbiology, Faculty of Medicine, University of Jember, Jember, Indonesia
Biochemistry, Genetics & Molecular Biology Education Health Professions Medicine & Pharmacology Nursing Public Health

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The Relationship between Milking Hygiene and Salmonella sp. Contamination on Cow’s Milk in Jember Regency Diana Chusna Mufida; Hammam Arif Shabri; Bagus Hermansyah; M Ali Shodikin; Enny Suswati
BKM Public Health and Community Medicine The 12th UGM Public Health Symposium
Publisher : Universitas Gadjah Mada

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Abstract

Purpose: Milk is a liquid produced by the mammary glands of female mammals. The nutritional content of milk almost covers the entire nutritional needs required by living things. The appearance of Salmonella sp. in cow's milk is due to milking methods that do not meet the standards, especially regarding milking hygiene management. This study examines the relationship between milking hygiene and Salmonella sp. contamination in cow's milk in Jember Regency. Method: This study used observational analytic research with a cross-sectional research design. Primary data were obtained through direct observation of milkers using a checklist related to milking. observe Salmonella sp. contamination using culture techniques on SCA (Salmonella Chromogenic Agar) as selective media agar for Salmonella sp. and Gram staining. Results: The results from this study show that the hygiene process pre-milking (78.9%), during-milking (71.1%), and post-milking (89.5%) have been implemented. The statistical test results showed a relationship between the pre-milking hygiene process with Salmonella sp. contamination (p-value < 0.05), but insignificant results were seen in the during and post-milking hygiene processes (p-value> 0.05). Conclusion: The conclusion from this study show that the three results only the hygiene processes pre-milking have results associated with Salmonella sp. contamination in cows' milk. Therefore, milkers are expected to maintain and improve dairy hygiene measures, especially before milking to prevent contamination of cow's milk.
High rate of multidrug-resistant Escherichia coli isolated from ready to eat: crispy fried chicken in Jember: a real threat to human health Enny Suswati; Supangat
BKM Public Health and Community Medicine The 12th UGM Public Health Symposium
Publisher : Universitas Gadjah Mada

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Purpose: This study was conducted to assess prevalence and antibiotic resistance profile of E. coli from ready-to-eat crispy fried chicken (CFC) in Jember city, Indonesia. Methods: This cross-sectional study was conducted from November 2020 to April 2021 by collecting a total of 81 CFC samples from 27 food stalls in campus area using a simple random sampling method. Isolation and determination of E. coli use were performed by conventional culture method and an antibiotic susceptibility test was conducted by using Kirby Bauer disk diffusion method on the Mueller–Hinton agar. Result: Out of 81 CFC samples, 77 (95.06%) were positive for E. coli. High E. coli drug resistance was observed on ampicillin, amoxicillin, (100%) followed by cefixime (98.72%), erythromycin (97.59%), sulfamethoxazole (93.59%), azithromycin (83.33%), cefotaxime (78.28%), chloramphenicol (75.64%), and cefixime (74.36%). On the other hand, there was the highest susceptibility for ciprofloxacin (64.10%). Multidrug resistance was also found in 76/77 (98.70%) of the isolates. MAR index was 0.4-1. Conclusion: The resistance pattern CfmCroAmlAmpAzmCtxSxtCE was the most common among the E. coli isolates, with 17 showing it. The multiple antibiotic index (MAR index) ranged from 0.4 to 1. Hygienic measures should be rigorously implemented, and monitoring resistance of E. coli is required to reduce the risks related to the emergence of multi-resistant bacteria.
Tinjauan Sistematik: Efektivitas dan Keamanan Fecal Microbiota Transplantation pada Infeksi Clostridium difficile Berulang pada Anak Fetri Rosdiana; Enny Suswati; Irawan Fajar Kusuma
Majalah Kedokteran Indonesia Vol 72 No 4 (2022): Journal of The Indonesian Medical Association - Majalah Kedokteran Indonesia, Vo
Publisher : PENGURUS BESAR IKATAN DOKTER INDONESIA (PB IDI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47830/jinma-vol.72.4-2022-520

Abstract

Background: Recurrent Clostridium difficile infection (RCDI) continues to increase in incidence and severity around the world. Fecal microbiota transplantation (FMT) is commonly used to treat RCDI in adults, but its efficacy and safety in the pediatric population are infrequently studied. Here, we performed a systematic review to evaluate the efficacy and safety of FMT for RCDI in children.Methods: Systematic literature search was performed using PubMed, Science Direct, Nature, Cochrane Library, and Springer to identify original studies in English language published from 2010-2020. The clinical resolution, bacterial resolution, and failure of therapy were calculated as indicators of efficacy of FMT. Adverse events were also calculated as indicators of FMT safety.Results: A total of 21 studies, with 498 pediatric patients were included in this review. There were 418/498 (84%) patients reported clinical and bacterial resolution. There were 67/498 (13%) patients who experienced FMT-related adverse events, with 97% of them had mild adverse event. There was one patient who reported FMT-related serious adverse events which were aspiration pneumonia and dehydration.Conclusion: We found evidence that supports the efficacy and safety of the use of FMT for treatment of recurrent C. difficile infection in children.
Co-Authors Bagus Hermansyah Diana Chusna Mufida Fetri Rosdiana Hammam Arif Shabri Irawan Fajar Kusuma M Ali Shodikin Supangat