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Karakterisasi Senyawa Bioaktif dan Aktivitas Antioksidan Ekstrak Kasar Caulerpa sp dan Gracilaria sp dari Perairan Bangkalan Madura Umi Nafisatul Qoyyimah; Hafiludin Hafiludin
Jurnal Kelautan Vol 18, No 3: Desember (2025)
Publisher : Department of Marine Sciences, Trunojoyo University of Madura, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/jk.v18i3.32623

Abstract

ABSTRAKRumput laut pada umumnya dapat dimanfaatkan sebagai sumber pangan dan juga sebagai antioksidan alami. Rumput laut yang berpotensi sebagai bahan aktioksidan alami dari perairan Bangkalan yaitu Caulerpa sp. dan Gracilaria sp. dikarenakan kandungan senyawa bioaktif di dalamnya. Penelitian ini bertujuan untuk mengetahui karakterisasi fisik dan kimia, kandungan senyawa bioaktif serta aktivitas antioksidan dari ekstrak kasar Caulerpa sp. dan Gracilaria sp. Penelitian dilaksanakan di Laboratorium Bioteknologi Laut, Universitas Trunojoyo Madura dengan beberapa tahap yaitu: preparasi sampel, analisa proksimat, analisa fitokimia dan analisis aktivitas antioksidan dengan DPPH. Hasil identifikasi morfologi Caulerpa sp memiliki warna hijau tua dan ramuli kecil seperti anggur. Gracilaria sp. memiliki warna merah kecoklatan dengan percabangan yang tidak teratur seperti akar serabut. Kadar proksimat Caulerpa sp dan Gracilaria sp. menunjukkan tingginya kadar air, abu, dan serat kasar, dibandingkan protein dan lemaknya. Ekstrak kasar rumput laut Caulerpa sp mengandung senyawa bioaktif berupa flavonoid, fenolik, steroid, terpenoid, alkaloid, saponin, dan tannin.  Ekstrak kasar rumput laut Gracilaria sp. senyawa bioaktif yaitu alkaloid, steroid, flavonoid, fenol, saponin, dan terpenoid. Aktivitas antioksidan terbaik terdapat pada ekstrak kasar rumput laut Caulerpa sp. pelarut n-heksan dengan nilai IC50 sebesar 116,41 ppm lebih kuat dibandingkan dengan ekstrak kasar rumput laut Gracilaria sp. Rumput laut dari perairan Bangkalan berpotensi dapat dikembangkan sebagai bahan bahan pangan alami dan antioksidan alami di masa yang akan datang.Kata Kunci: Caulerpa sp, Gracilaria sp, senyawa bioaktif, dan aktivitas antioksidan. ABSTRACTSeaweed in general can be used as a food source and also as a natural antioxidant. Seaweed that has the potential as a natural antioxidant from Bangkalan waters is Caulerpa sp. and Gracilaria sp. due to the content of bioactive compounds in it. This research aims to determine the physical and chemical characteristics, content of bioactive compounds and antioxidant activity of crude extracts of Caulerpa sp. and Gracilaria sp. The research was carried out at the Marine Biotechnology Laboratory, Trunojoyo University, Madura in several stages, namely: sample preparation, proximate analysis, phytochemical analysis and antioxidant activity analysis with DPPH. The results of the morphological identification of Caulerpa sp have a dark green color and small ramuli like grapes. Gracilaria sp. has a brownish red color with irregular branching like fibrous roots. Proximate levels of Caulerpa sp and Gracilaria sp. shows high levels of water, ash and crude fiber, compared to protein and fat. Caulerpa sp seaweed crude extract contains bioactive compounds in the form of flavonoids, phenolics, steroids, terpenoids, alkaloids, saponins and tannins. Crude extract of seaweed Gracilaria sp. Bioactive compounds are alkaloids, steroids, flavonoids, phenols, saponins and terpenoids. The best antioxidant activity is found in the crude extract of seaweed Caulerpa sp. with n-hexane solvent was an IC50 value of 116.41 ppm is stronger than the crude extract of seaweed Gracilaria sp. Seaweed from Bangkalan seawaters has the potential to be developed as a natural food ingredient and natural antioxidant in the future.Keywords: Caulerpa sp, Gracilaria sp, bioactive compounds, and antioxidant activity.
KARAKTERISTIK MIKROPLASTIK PADA SUMBER AIR BAKU DAN SEDIMEN TAMBAK GARAM DI PAMEKASAN DENGAN METODE FT-IR Silfi Maulidatur Rohmah; Haryo Triajie; Hafiludin Hafiludin; AB Chandra
JURNAL AKUAKULTUR, TEKNOLOGI DAN MANAJEMEN PERIKANAN TANGKAP, ILMU KELAUTAN Vol 8 No 1 (2025): JOURNAL OF INDONESIAN TROPICAL FISHERIES
Publisher : Fakultas Perikanan dan Ilmu Kelautan Universitas Muslim Indonesia Makassar, Sulawesi Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/joint-fish.v8i1.572

Abstract

Plastic in the aquatic environment poses a new problem, namely microplastics. Microplastics are widespread in marine waters and sediments. Seawater has been widely used by humans, one of which is as raw water for making salt, but the presence of microplastics in marine waters is feared to affect the quality of salt production. The aim of this study was to analyze water quality and identify the quantity and type of microplastic polymers in raw water sources of salt ponds and sediments in Pamekasan. Water quality data for pH, temperature, and DO in Pamekasan salt ponds were in the ranges of 7.5 ± 0.02 - 7.8 ± 0.02; 29.3 ± 0.03 - 31.9 ± 0.09 °C; 6.83 ± 0.26 - 9.42 ± 0.21 mg/L. Microplastics found in salt pond raw water reached 2.06-3.32 particles / L. Microplastics found in sediments were 2,65 ± 0,14 partikel/L and 0,82 ± 0,02 particles/gram. The forms of microplastics found are fibers, fragments, and films. The microplastics in water and sediments influence as much as 44,58% or quite significantly. The types of polymers found in the samples are Polypropylene (PP), Polyethylene (PE), Polyamide (PA), and Low-Density Polyethylene (LDPE).