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All Journal Juvenil: Jurnal Ilmiah Kelautan dan Perikanan
Christian Larbi Ayisi
University of Environment and Sustainable Development
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Environmental Science

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Transfection Of Difficult-To-Transfect Zebrafish (Danio rerio) ZF4 Cells Using Chemical Transfection and Nucleofection Method Rizka Rahmana Putri; Christian Larbi Ayisi
Juvenil Vol 4, No 2: Mei 2023
Publisher : Department of Marine and Fisheries, Trunojoyo University of Madura, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/juvenil.v4i2.19586

Abstract

ABSTRACTTransfection is a powerful tool for introducing foreign DNA into target cells. Many kits and methods are offered to simplify the process of introducing foreign DNA into cells. ZF4 is a cell type derived from zebrafish that is difficult to transfect. The majority of studies involving transfection into cells require transfection efficiency 70%. Our research demonstrates transfection using 2 kinds of methods, namely chemical transfection using X-tremeGene HP and electroporation-based transfection using a nucleofector device which will later be called Nucleofection. Our results show that transfection using electricity (Nucleofection) provides better transfection efficiency than chemical transfection (X-tremeGene HP).Keywords: maxGFP, nucleofection, transfection, zebrafish (Danio rerio), ZF4 cells
Co-Authors Rizka Rahmana Putri