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All Journal Jurnal Ilmu Pertanian jurnal Ilmu Pertanian (Agricultural Science) Jurnal Ilmu-Ilmu Pertanian
Miranti Dian Pertiwi, Miranti Dian
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Agriculture, Biological Sciences & Forestry

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Effects of explants and culture medium compositions on quality of chrysanthemum ‘Jayanti Agrihorti’ rooted cuttings Shintiavira, Herni; Pramanik, Dewi; Daniyanti, Ratna Dewi; Pertiwi, Miranti Dian; Rachmawati, Fitri
Jurnal Ilmu Pertanian Vol 9, No 2 (2024): August
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.95898

Abstract

'Jayanti Agrihorti' is a superior chrysanthemum therefore rooted cuttings quality is required. In vitro propagation increases the rate of propagation and produces true-to-type plants. The research to obtain the best explants and culture medium composition that is capable of producing high-quality plants.  The research was arranged in a randomized complete block design consisting of two factors. The first factor was the explant, including the apical shoot of 0.5 cm, 1.0 cm, and the nodal segment. The second factor was in vitro culture medium composition (CP), consisting of  CP1 (Murashige and Skoog (MS) medium for initiation, followed by MS + 2.5 mg.L⁻¹ gibberellic acid (GA₃) for subculture), CP2 (MS + 0.25 mg.L⁻¹ benzyl amino purine (BAP) for initiation, followed by MS for subculture), CP3 (MS + 0.25 mg.L⁻¹ BAP for initiation, followed by MS + 2.5 mg.L⁻¹ GA₃ for subculture), and CP4 (MS + 0.5 mg.L⁻¹ BAP for initiation, followed by MS + 0.25 mg.L⁻¹ BAP for subculture). Acclimatization was performed after third subculture. The apical shoot size of 0.5−1.0 cm is optimum for producing chrysanthemum-rooted cuttings. Meanwhile, MS medium for initiation stage, followed by MS + 2.5 mg.L⁻¹ GA₃ for subculture is the best culture medium composition for in vitro propagation. This explant and culture medium composition produced higher chlorophyll a, b, and a+b content, thereby resulting in higher plant, more leaves, larger stem diameter, and longer root length. This are recommended for chrysanthemum propagation, particularly in 'Jayanti Agrihorti'.
Pengaruh Pola Tanam Terhadap Pertumbuhan dan Hasil Kentang di Wilayah Dataran Tinggi Dieng, Jawa Tengah Pertiwi, Miranti Dian; Cempaka, Intan Gilang
Jurnal Ilmu-Ilmu Pertanian Vol. 27 No. 1 (2020): Juli
Publisher : Politeknik Pembangunan Pertanian Yogyakarta-Magelang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55259/jiip.v27i1.75

Abstract

Peluang peningkatan produktivitas kentang di Jawa Tengah masih sangat terbuka, mengingat produktivitasnya belum maksimal. Salah satu penyebabnya diduga adalah frekuensi penanaman kentang yang terlalu sering, yaitu dua sampai tiga kali dalam satu tahun dengan pola kentang-kentang-kentang atau sayuran lain-kentang-kentang. Oleh karena itu dilakukan percobaan dengan tujuan mengidentifikasi perbedaan pertumbuhan dan hasil kentang yang ditanam di tiga wilayah dataran tinggi Dieng yang memiliki pola tanam yang berbeda dalam setahun. Percobaan disusun dengan rancangan petak terbagi, perbedaan pola tanam di tiga lokasi sebagai petak utama (Wonosobo 2 kali tanam kentang/tahun, Banjarnegara 3 kali tanam kentang/tahun, Pekalongan 1 kali tanam kentang/tahun) dan tiga varietas sebagai anak petak (Granola L, Granola K, Tedjo-MZ) yang disusun dalam tiga ulangan. Hasil penelitian menunjukkan bahwa pertumbuhan tiga varietas lebih optimal di lokasi Pekalongan dibanding di Wonosobo dan Banjarnegara. Demikian juga dengan komponen hasil yaitu bobot segar umbi per tanaman dan produktivitas umbi per hektar menunjukkan bahwa di lokasi Pekalongan nyata lebih tinggi dibanding di Wonosobo dan Banjarnegara. Hasil percobaan ini memberikan informasi awal bahwa perbedaan pola tanam atau intensitas tanaman kentang dalam setahun berpengaruh terhadap pertumbuhan dan hasil yang diperoleh. Pola tanam kentang satu kali setahun menunjukkan pertumbuhan dan hasil yang lebih baik dibanding pola tanam kentang dua dan tiga kali setahun.
Effects of explants and culture medium compositions on quality of chrysanthemum ‘Jayanti Agrihorti’ rooted cuttings Shintiavira, Herni; Pramanik, Dewi; Daniyanti, Ratna Dewi; Pertiwi, Miranti Dian; Rachmawati, Fitri
Jurnal Ilmu Pertanian Vol 9, No 2 (2024): August
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.95898

Abstract

'Jayanti Agrihorti' is a superior chrysanthemum therefore rooted cuttings quality is required. In vitro propagation increases the rate of propagation and produces true-to-type plants. The research to obtain the best explants and culture medium composition that is capable of producing high-quality plants.  The research was arranged in a randomized complete block design consisting of two factors. The first factor was the explant, including the apical shoot of 0.5 cm, 1.0 cm, and the nodal segment. The second factor was in vitro culture medium composition (CP), consisting of  CP1 (Murashige and Skoog (MS) medium for initiation, followed by MS + 2.5 mg.L⁻¹ gibberellic acid (GA₃) for subculture), CP2 (MS + 0.25 mg.L⁻¹ benzyl amino purine (BAP) for initiation, followed by MS for subculture), CP3 (MS + 0.25 mg.L⁻¹ BAP for initiation, followed by MS + 2.5 mg.L⁻¹ GA₃ for subculture), and CP4 (MS + 0.5 mg.L⁻¹ BAP for initiation, followed by MS + 0.25 mg.L⁻¹ BAP for subculture). Acclimatization was performed after third subculture. The apical shoot size of 0.5−1.0 cm is optimum for producing chrysanthemum-rooted cuttings. Meanwhile, MS medium for initiation stage, followed by MS + 2.5 mg.L⁻¹ GA₃ for subculture is the best culture medium composition for in vitro propagation. This explant and culture medium composition produced higher chlorophyll a, b, and a+b content, thereby resulting in higher plant, more leaves, larger stem diameter, and longer root length. This are recommended for chrysanthemum propagation, particularly in 'Jayanti Agrihorti'.
Co-Authors Cempaka, Intan Gilang Daniyanti, Ratna Dewi Fitri Rachmawati Herni Shintiavira Pramanik, Dewi