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All Journal JURNAL TEKNOLOGI LINGKUNGAN Caraka Tani: Journal of Sustainable Agriculture Biosaintifika: Journal of Biology & Biology Education
Yohanes Bernard Subowo, Yohanes Bernard
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Agriculture, Biological Sciences & Forestry Environmental Science

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PENGUJIAN PUPUK HAYATI KALBAR UNTUK MENINGKATKAN PRODUKTIVITAS TANAMAN KEDELAI (Glycine max) var. BALURAN Subowo, Yohanes Bernard; Sugiharto, Arwan; Suliasih, Suliasih; Widawati, Sri
Caraka Tani: Journal of Sustainable Agriculture Vol 25, No 1 (2010): March
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (57.022 KB) | DOI: 10.20961/carakatani.v25i1.15756

Abstract

Research aimed to evaluate the ability of kalbar biofertilizer to improve productivity of soybean. Experiment was conducted in the field experimental plot of Cibinong Science Centre. Soybean (Glycine max) var Baluran was treated with a series of fertilizer i.e.: compost containing Nitrogen fixing microbes, compost containing Phosphate solubilizing microbes, compost containing lignocelullosic degrading fungus, Kalbar biofertilizer (compost containing Nitrogen fixing, Phosphate solubilizing and lignocellulosic degrading microbes), chemist fertilizer, compost and control. The height of plant, fresh weight of biomass, number of leaves, pod number, and weight of seed were observed. The Nitrogen, Phosphate and Carbon which exist on the soil observed before planting, while flowering and while harvesting. The result was the application of kalbar biofertilizer able to increase the Carbon (C), Nitrogen (N) and Phosphor (P) content in the soil. This treatment also increased the fresh weight of biomass (22%), the number of pods (11, 11%) and the weight of seed (12,22%).
The Interaction between Marasmius pulcheripes J8 and Soil Fungi on Laccase Activity for POME Degradation Subowo, Yohanes Bernard; Sugiharto, Arwan
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 3 (2019): Article-in-Press
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i3.20827

Abstract

A study on the effect of Marasmius  pulcheripes J8 and soil fungi interaction on the activity and ability of laccase to degrade palm oil mill effluent (POME) was previously conducted, and some Basidiomycetes fungi were identified capable. Therefore, the aim of this study was to determine the ability of Marasmius pulcheripes J8 to degrade POME in the presence of inducers, and interactions with soil fungi. Furthermore, 3 types of inducers were applied to elevate its laccase activity, which include CuSO4, sucrose and Ammonium tartrate. In addition, M. pulcheripes J8 was grown together with soil fungi, encompassing Aspergilus niger NK and Penicillium sp R 75, in order to boost the action. The results showed the highest laccase activity was in M. pulcheripes J8 pure culture on a PDB medium of 3566.04 U / mL.  Moreover, the POME decolorization was up to 74.25% after 20 days of incubation, and reduced COD level was 81%. Meanwhile, the addition of an inducer has never been performed before, and the outcome of this investigation showed the ability of M. pulcheripes J8 to degrade POME, and decrease environmental pollution. POME waste treatment using fungi is more affordable than other methods.
SELEKSI JAMUR TANAH PENDEGRADASI SELULOSA DAN PESTISIDA DELTAMETHRIN DARI BEBERAPA LINGKUNGAN DI KALIMANTAN BARAT Subowo, Yohanes Bernard
Jurnal Teknologi Lingkungan Vol. 13 No. 2 (2012)
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (514.836 KB) | DOI: 10.29122/jtl.v13i2.1421

Abstract

Telah dilakukan penelitian mengenai seleksi jamur tanah pengurai selulosa dan deltamethrin dari beberapa lingkungan di Kalimantan Barat. Tujuan penelitian untuk memperoleh isolat jamur yang mampu menguraikan selulosa dan pestisida deltamethrin.  Sampel tanah diambil dari beberapa lingkungan ekstrim di Kalimantan Barat meliputi: tanah gambut, tanah kering, tanah pantai, tanah pertanian dan tanah mangrove. Setelah dilakukan isolasi diperoleh 79 nomor isolat. Sebanyak 72 isolat dapat membentuk clear zone pada media mengandung CMC 1%. Sejumlah 10 isolat membentuk clear zone  berukuran besar. Jamur Aspergillus niger PS 1.4 dapat tumbuh paling baik pada media mengandung CMC 1% dengan menghasilkan bobot biomassa paling tinggi (0,7 g/L media). Jamur ini mempunyai aktivitas enzim selulase sebesar 0, 127 unit/ ml. Jamur Aspergillus niger PS 1.4 juga tumbuh pada beberapa pestisida: 50mg/L (ppm) Clorpirifos, 50 mg/L Cypermethrin dan 50mg/L Deltamethrin. Jamur Aspergillus niger PS1.4 dapat mendegradasi Deltamethrin sebanyak 90,2% dalam waktu 10 hari. Kata kunci: Aspergillus niger, deltamethrin, jamur tanah, penguraian, selulosa AbstractA research on selection of cellulose and deltamethrin degrading soil fungi from some environments in West Kalimantan had been done. The aim was to obtain isolates of fungi that have a high ability on decomposing cellulose and deltamethrin. The soil sample was taken from some environments in West Kalimantan, included: peatland,heath forest soil, sediment of manggrove, and coastal soil. Seventy two isolates were able to hydrolize CMC (Carboxy Methyl Cellulose). Aspergillus niger PS 1.4 was able to grow fastest among strains tested and yielded highest of mycelium. The fungi has cellulase activity was 0,127 unit/ml and able to grow on some pesticides also, included: 50 ppm Chlorpirifos, 50 ppm Cypermethrin and 50 ppm Deltamethrin. Aspergillus niger PS 1.4 was able to degrade deltamethrin as much as 90,2% in 10 days. Key words: Aspergillus niger, cellulose, deltamethrin, degradation, soil fungi
JAMUR PEMBENTUK GAHARU SEBAGAI PENJAGA KELANGSUNGAN HIDUP TANAMAN GAHARU (Aquilaria sp) Subowo, Yohanes Bernard
Jurnal Teknologi Lingkungan Vol. 11 No. 2 (2010)
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (382.715 KB) | DOI: 10.29122/jtl.v11i2.1200

Abstract

Agarwood is economical important non forest timber product. The objective of this research was to obtain fungi that have ability to effectively infect and form agarwood.The fungi were isolated and selected from agarwood for effective infection studies.Ten isolates were successfully isolated, they were belonging to Aspergillus (JG5, JG17,KT17, JG31, JG32, JG21, JG19), Penicillium (JG4), Fusarium (JG12, JG18). Four isolates were successful form effective infection and they identified as Aspergillus sp JG17, Aspergillus sp JG31, Aspergillus sp JG32 and Fusarium sp JG12. They produced secondary metabolite. The Fusarium sp JG12 was the most effective strain produced agarwood and form resin gaharu on Aquilaria beccariana after 4 months inoculation. The size of agarwood formed was 2,96 cm in length and 0,16 cm wide. It is recommendedto use this strain for effective formation of agarwood.Key words: isolation, fungi, agarwood, Fusarium
The Influence of Inducers on the Coltricia cinnamomea Laccase Activity and its Ability to Degrade POME Subowo, Yohanes Bernard; Sugiharto, Arwan
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 2 (2021): August 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i2.29660

Abstract

Some species of Basidiomycetes, specifically white rot groups, produce three ligninolytic enzymes, namely, Lignin Peroxidase (LiP), Manganese Peroxidase (MnP) and Laccase (Lac), which have low activity in degrading Palm Oil Mill Effluent (POME). The research objective was to obtain the data on the ability of the Coltricia cinnamomea to produce LiP, MnP, and Lac enzymes to degrade POME. This research also studied the effect of sucrose, alcohol, veratryl alcohol, CuSO4 and ZnSO4,as inducers. Isolates of Coltricia cinnamomea, which were stored in a PDA media at -20℃ were obtained from the Microbiology section of the Research Center for Biology (LIPI). Furthermore, the growth media used were DM, Bean sprout Extract (TE) and PDB. The result indicated that PDB is the most suitable growth media for the production of ligninolytic enzymes, because in this medium these enzymes showed the highest activity. It was also observed that sucrose increased the laccase activity by 40.80%. Furthermore, Coltricia cinnamomea was able to reduce the concentration of Poly R-478 by 60.74%, after the addition of ZnSO4. In addition, it degraded and decreased the color and COD of POME, by 72.63% and 91.19% respectively, after the addition of veratryl alcohol, and incubation for 10 days. Therefore, this fungus can be used to degrade POME in order to prevent environmental pollution. Coltricia cinnamomea has not been used for POME degradation. By using Coltricia cinnamomea, we  obtained new data regarding the activity of laccase and its ability to degrade POME. 
Co-Authors Arwan Sugiharto Sri Widawati Suliasih Suliasih