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All Journal ILMU KELAUTAN: Indonesian Journal of Marine Sciences Jurnal Kelautan Tropis
Ervia Yudiati
Department of Marine Science, Faculty of Fisheries and Marine Sciences, Universitas Diponegoro
Earth & Planetary Sciences

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Carrageenan Extraction of Kappaphycus alvarezii Seaweed from Nusa Lembongan Waters Using Different Alkaline Treatments Ichoun Brawici Dhewang; Ervia Yudiati; Subagiyo Subagiyo; Rabia Alghazeer
Jurnal Kelautan Tropis Vol 26, No 2 (2023): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v26i2.17389

Abstract

Kappaphycus alvarezii is a carrageenan-producing red seaweed that is widely cultivated in Nusa Lembongan waters, Bali, Indonesia. Carrageenan is generally extracted using an alkaline base,. iIn this study three different types of alkali were used to extract carrageenan originating from Nusa Lembongan Waters. This study aims to determine the quality of the extraction. The three alkalis used were KOH, NaOH, Ca(OH)2, and distilled water as a control. The 20 g dried seaweed was weighed, washed with the tap running water, and immersed in 0.15% alkaline solution (KOH, NaOH, Ca(OH)2) as well as distilled water for 24 hrs. Followed by rinsing with running water until pH was neutral. The solution was soaked and heated at 100ºC for 2 hours with a ratio of seaweed and water 1 kg in 20 L, and filtered. The extract was mixed with 1.25% KCI. The carrageenan precipitate was put in the oven at 60-80ºC until dry for 48 hours. The yield test results showed that the use of alkaline Ca(OH)2 resulted in a yield of 29.28% better than alkaline KOH (28.18%) and NaOH (27.7%). Based on the analysis of Fourier Transform Infrared Spectroscopy (FTIR), extraction using alkaline Ca(OH)2 showed functional groups characteristic of iota-carrageenan, whereas using alkaline KOH and NaOH showed functional groups characteristic of kappa-carrageenan.
Immune Profile of Litopenaeus vannamei in Monoculture and IMTA Ponds System Chrisna Adhi Suryono; Ervia Yudiati; Nuril Azhar
Jurnal Kelautan Tropis Vol 26, No 2 (2023): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v26i2.18428

Abstract

Integrated Multi Trophic Level (IMTA) is identified as the integrating culture of multi-species in one area. The effectiveness of space and feed is the main aspect from this point. The disease and health of shrimp management on shrimp production including immune boosters plays a critical aspect. The aim of this research is to determine and analyze the Litopenaeus vannamei immune system in different culture systems, namely polyculture (IMTA) and monoculture. Alginate was applied as supplementation diet by oral administration. The cellular and humoral immune parameters ie. Total hemolymph Count (THC), Phagocyte Activity (PA), Phagocyte Index (PI), Phenoloxidase activity (PO), and Superoxide dismutase activity (SOD) were assessed, monthly. A completely randomized design (CRD) with two treatments (IMTA and monoculture, and four replications in 500 m2 pond area was conducted. In the third month, THC and PA of shrimp from the monoculture pond resulted in significant differences (p<0.05) compared to polyculture (IMTA). SOD activity from monoculture is significantly higher (p<0.05) at every sampling period. In PO enzyme activity of shrimps from monoculture pond produced higher results compared to IMTA ponds in the 2nd and 3rd months. It is concluded that L. vannamei monoculture ponds produced higher immune parameters compared to IMTA ponds. The food competition in terms of feed and space from IMTA ponds may affect this fact. Improving the feeding technique is recommended.
Alginate and Spirulina Vast-Promoting Immune Activity and Resistance to Diverse Vibrio Species in Litopenaeus vannamei Culture Ervia Yudiati; Agus Trianto; Sarjito Sarjito; Diah Ayu Satyari Utami; Murni Karim; Nuril Azhar
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 31, No 1 (2026): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.31.1.49-60

Abstract

Litopenaeus vannamei is a key species in global aquaculture; however, its susceptibility to vibriosis presents a persistent challenge. The aim of this study was to investigate the effects of dietary supplementation with alginate and Spirulina hot water extract (SWE) on the immune response and gene expression associated with pathogen resistance. Shrimp fed diets containing 3 g·kg⁻¹ of alginate and 5 mg·kg⁻¹ of SWE exhibited marked improvements in immune parameters, including total hemocyte count, phagocytic activity, phagocytic index, phenoloxidase activity, and superoxide dismutase levels, as measured on days 3 and 7 (P< 0.05). Following challenge with Vibrio parahaemolyticus, V. vulnificus, and V. harveyi, treated shrimp demonstrated a 90% survival rate compared to 0% in the control group. Gene expression analysis revealed a 2-fold and 1.5-fold upregulation of prophenoloxidase (proPO) and lipopolysaccharide β-glucan binding protein (LGBP), respectively, indicating the activation of the proPO cascade and pathogen recognition pathways. These results provide direct evidence that alginate and SWE act as immunostimulants by modulating hemocyte function and immune-related gene expression. The findings of the present study suggest that oral supplementation with these natural bioactive compounds can serve as a functional dietary strategy to enhance disease resilience in shrimp and promote more sustainable aquaculture systems, in seven days period.
Co-Authors Agus Trianto Chrisna Adhi Suryono Diah Ayu Satyari Utami Ichoun Brawici Dhewang Murni Karim Nuril Azhar Nuril Azhar Rabia Alghazeer Sarjito Sarjito Subagiyo Subagiyo