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All Journal Buletin Veteriner Udayana
I Gusti Ngurah Kade Mahardika
Laboratorium Biomedik dan Biologi Molekuler Fakultas Kedokteran Hewan, Universitas Udayana, Jl. Raya Sesetan Gang Markisa 6A, Denpasar, Bali, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Pengembangan Vaksin Vektor Adenovirus untuk Virus African Swine Fever Berbasis Protein Non-Struktural Made Sumitha Kameswari; I Gusti Ngurah Kade Mahardika; Ida Bagus Kade Suardana
Buletin Veteriner Udayana Vol. 15 No. 5 October 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i05.p23

Abstract

African swine fever (ASF) is a viral disease of swine which causes high mortality as well as causes high economic losses in domestic pig. This study describes the process to develop an adenovirus vectors ASF vaccine with A224L and A276R coding region and its immune response in mice. Synthetic DNA containing the A224L and A276R genes was amplified and cloned into the commercial plasmid pAdenoX with an infusion cloning kit. A total of 106 AdenoX-ASF-224L-276R viruses from transfected T293 cells were injected into six mice intra-muscularly, while the other six mice were given T293 cell extract and were treated as controls. Blood without anticoagulant was collected from the facial vein of mice at 1, 3, 5, and 7 weeks after injection. Antibodies against ASF were tested by ELISA using synthetic peptides of A224L and A276R as antigens. AdenoX-ASF_A276R_A224L recombinant was successfully generated and proven. Injection of recombinant AdenoX-ASF_A276R_A224L in mice can trigger an immune response at week 7 significantly higher (p=0.00) compared to control, while weeks 1, 3 and 5 are not different from control (p>0.05). Production of recombinant adenovirus in monolayer cells and virus quantification need to be improved. Booster testing needs to be done after the third week.
Co-Authors Ida Bagus Kade Suardana Made Sumitha Kameswari