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All Journal Jurnal Perikanan dan Kelautan Jurnal Perikanan
Limin Santoso
Aquaculture Study Program, Faculty of Agriculture, University of Lampung
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Environmental Science Immunology & microbiology Social Sciences

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The Use of Indigofera Indigofera zollingeriana (Miquel, 1855) Leaf Meal as Feed Raw Material for Growth of Hoven’s Carp Fry Leptobarbus hoevenii (Bleeker, 1851) Emilda Agustina; Limin Santoso; Ediwarman Ediwarman; Paino Paino; Supono Supono
Jurnal Perikanan dan Kelautan Vol 28, No 2 (2023): June 2023
Publisher : Faculty of Fisheries and Marine, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.28.2.151-159

Abstract

Indigofera leaf meal can be used as an alternative raw material and has high potential as a source of vegetable protein for Hoven’s carp fry to reduce the use of imported raw materials. The purpose of this study was to examine the effect of using indigofera leaf meal with different combinations of feed as feed raw materials on growth performance and survival of Hoven’s carp (Leptobarbus hoevenii) fry. This study was carried out from July to August 2021 at the freshwater aquaculture center (BPBAT) Sungai Gelam Jambi. This study method used a completely randomized design (CRD) with 3 treatments and 3 replications, i.e: control (commercial feed 100%), treatment 1 (commercial feed 50%+ indigofera leaf meal 50%), and treatment 2 (indigofera leaf meal 100%). The data obtained were analyzed by Anova variance and further tested by Duncan's test. Based on the analysis of variance, the use of Indigofera zollingeriana leaf meal had a significant effect (P<0.05) on absolute weight growth, absolute length, specific growth rate, and feed conversion ratio, while survival rates were not significantly different (P >0.05). The results showed that the increasing proportion of the use of indigofera le
ENZYMATIC ACTIVITY OF LACTIC ACID BACTERIA ISOLATES FROM THE GASTROINTESTINAL TRACT OF NILE TILAPIA (Oreochromis niloticus) FROM LAKE RANAU, WEST LAMPUNG AS PROBIOTIC CANDIDATES Hilma Putri Fidyandini; Rika Iwan Syahputri; Agus Setyawan; Limin Santoso
Vol 16 No 3 (2026): JURNAL PERIKANAN
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jp.v16i3.2243

Abstract

Tilapia (Oreochromis niloticus) cultivated in Lake Ranau, West Lampung, possesses several superior characteristics, including good growth performance, thick flesh, savory taste, and the absence of muddy odor. These advantages are presumed to be associated with the presence of gut microbiota, particularly lactic acid bacteria (LAB), which have potential as probiotics. This study aimed to obtain lactic acid bacteria isolates from the gastrointestinal tract of tilapia, evaluate their enzymatic activities, and determine potential probiotic candidates. The research employed an exploratory method through bacterial isolation, LAB screening using de Man Rogosa Sharpe Agar (MRSA), enzymatic activity assays, morphological and biochemical identification, and molecular analysis based on 16S rDNA sequencing. The results showed that 12 bacterial isolates were successfully grown on MRSA medium. Ten isolates exhibited amylase activity, while all isolates demonstrated lipase and protease activities, indicated by the formation of clear zones around the bacterial colonies. Isolate UNR.11 showed the highest enzymatic activities with clear zone diameters of 25.5 ± 2.80 mm for amylase, 20.4 ± 1.2 mm for lipase, and 22.7 ± 4.06 mm for protease activity. Based on macroscopic, microscopic, biochemical, and 16S rDNA sequence analyses, isolate UNR.11 was identified as Bacillus cereus with 99% similarity. This isolate has potential as a probiotic candidate due to its ability to produce digestive enzymes, namely amylase, lipase, and protease, which may support digestion and nutrient utilization in fish.
Co-Authors Agus Setyawan Ediwarman Ediwarman Emilda Agustina Hilma Putri Fidyandini Paino Paino Rika Iwan Syahputri Supono Supono