Article Per Year (5 Year)
p-Index From 2021 - 2026
0.817
P-Index
This Author published in this journals
All Journal Biodiversitas Journal of Biological Diversity
Sri Suharti
Animal Nutrition And Feed Technology, Animal Science, IPB University. Jl. Agatis, Kampus Fapet IPB Dramaga,Bogor, 16680, Jawa Barat, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Published : 5 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 5 Documents
Search

 1 
Phenotypic identification, nutrients content, bioactive compounds of two jengkol (Archidendron jiringa) varieties from Bengkulu, Indonesia and their potentials as ruminant feed NUR HIDAYAH; RUKIAH LUBIS; KOMANG G WIRYAWAN; SRI SUHARTI
Biodiversitas Journal of Biological Diversity Vol. 20 No. 6 (2019)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d200624

Abstract

Abstract. Hidayah N, Lubis R, Wiryawan KG, Suharti S. 2019. Phenotypic identification, nutrients content, bioactive compounds of two jengkol (Archidendron jiringa) varieties from Bengkulu, Indonesia and their potentials as ruminant feed. Biodiversitas 20: 1671-1680. Agricultural waste is abundant in tropical countries. Many farmers in these countries have been using this waste as the main sources for feeding livestock. Ones of them are jengkol (Archidendron jiringa) by-product like peels and leaves have not been utilized optimally. The aims of this study were to explore the peel and leaves of two varieties of jengkol, i.e., jengkol gajah and jengkol padi, from Bengkulu Province and to assess their potentials as ruminant feed. Variables observed were phenotypic identification, nutrients content, and bioactive compounds. Phenotypic identification was completed using non-experimental examination through survey and observation methods to identify the phenotypic characters of jengkol in four districts in Bengkulu. The peel of jengkol padi had a blackish purple color and was thicker than that of jengkol gajah. The leaves of jengkol gajah was longer (10.2-15.5 cm), but jengkol padi had wider leaves (6-7.5 cm). The proportion of weight of jengkol peel (59.99%) is higher than seed (40.01%). Jengkol peel had high fiber content (33.07-35.28%) while the leaves were rich in protein and total digestible nutrient (TDN) (15.17-19.26% and 63.87-65.82%). In nutrient content comparison, the peel of jengkol padi was better (crude protein (CP:8.41%, ether extract (EE):0.79%, crude fiber (CF): 35.28% and total digestible nutrient (TDN): 52.81% but jengkol gajah had better nutrient in its leaves (CP:19.26%, EE:2.50%, CF:26.66% and TDN:51.56%).The saponin content (26.52%), total phenol (2.99%), and tannin (1.22%) in the peel were higher than those in the leaves, while the leaves had higher flavonoids content (2.0%) than the peels. Bioactive compounds in jengkol gajah were higher than those in jengkol padi. Based on this study, both jengkol gajah and jengkol padi are potential as ruminant feed in which the peel can be a source of fiber and saponins, while the leaves are potentially used as a source of protein, fiber and saponins.
The enrichment process and morphological identification of anaerobic fungi isolated from buffalo rumen SINTA AGUSTINA; KOMANG GEDE WIRYAWAN; SRI SUHARTI; ANJA MERYANDINI
Biodiversitas Journal of Biological Diversity Vol. 23 No. 1 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230150

Abstract

Abstract. Agustina S, Wiryawan KG, Suharti S, Meryandini A. 2021. The enrichment process and morphological identification of anaerobic fungi isolated from buffalo rumen. Biodiversitas 23: 469-477. Anaerobic fungi are one of the microbes that have an important role in rumen fiber degradation because they can produce cellulase enzymes and penetrate feed particles. Nevertheless, few studies were performed to test the potential of anaerobic fungi in Indonesia. Therefore, the present study was carried out to evaluate the impact of the enrichment process on pH value, the zoospores population, NH3 (ammonia) concentration, and VFA (Volatile Fatty Acid) proportion. In addition, this research was also performed to isolate anaerobic fungi from buffalo rumen and identify their morphological characteristics. The enrichment stage of anaerobic fungi was carried out using the Hungate method. Results showed that the population of fungi zoospores, pH value, ammonia concentration, the proportion of acetate, and total VFA were significantly affected by the incubation time (P<0.01). In addition, Caecomyces, Neocallimastix, and Piromyces were rumen anaerobic fungi isolated from buffalo rumen with different morphological characteristics. It can be concluded that the incubation time increased the zoospore population, the concentration of NH3, acetate proportion, and total VFA but decreased media's pH value.
Molecular identification of anaerobic fungi isolated from buffalo rumen with their growth rate, cellulase enzyme activity, and fermentation products characteristics SINTA AGUSTINA; KOMANG GEDE WIRYAWAN; SRI SUHARTI; ANJA MERYANDINI
Biodiversitas Journal of Biological Diversity Vol. 23 No. 12 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d231243

Abstract

Abstract. Agustina S, Wiryawan KG, Suharti S, Meryandini A. 2022. Molecular identification of anaerobic fungi isolated from buffalo rumen with their growth rate, cellulase enzyme activity, and fermentation products characteristics. Biodiversitas 23: 6448-6455. Anaerobic fungi are essential microbes in the degradation process of feed fiber in the rumen with the ability to produce fibrolytic enzymes and rhizoids that can penetrate feed particles. The activity of cellulase enzymes in fungi is influenced by several factors such as the type of feed and livestock used as a source of isolate. Therefore, this research aimed to analyze the types of anaerobic fungi isolated from buffalo based on their DNA nitrogenous bases composition and also to evaluate the growth rate, cellulase enzyme activity, and fermentation product concentration of anaerobic fungi. The growth rate of fungi was examined by measuring the biomass formed, while cellulase enzyme activity was carried out using CMC, Avicel, and Filter Paper as substrates. The fungal fermentation products were analyzed using the HPLC and GC methods. The results showed that the fungi isolated from buffalo rumen were closely related to anaerobic fungi type Piromyces sp., Caecomyces sp., and Neocallimastix frontalis, with different growth rate, cellulase enzyme activity, and the fermentation products concentration in each type of anaerobic fungi. Therefore, it can be concluded that anaerobic fungi isolated from buffalo rumen can degrade cellulose. It was also discovered that fungi Neocallimastix frontalis had a higher growth rate, cellulase enzyme activity (CMCase, avicelase, and FPase), and fermentation products than Piromyces sp. and Caecomyces sp.
Short Communication: Morphological, biochemical, and molecular identification of cellulolytic bacteria isolated from feces of endemic tropical herbivores SRI SUHARTI; NUR NOVRARIANI; KOMANG G. WIRYAWAN
Biodiversitas Journal of Biological Diversity Vol. 24 No. 7 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240742

Abstract

Abstract. Suharti S, Novrariani N, Wiryawan KG. 2023. Short Communication: Morphological, biochemical, and molecular identification of cellulolytic bacteria isolated from feces of endemic tropical herbivores. Biodiversitas 24: 4046-4051. Indonesia has endemic herbivores that consume lignocellulose feedstuffs including grass, tree foliage, rice straw, and legume, indicating the presence of cellulolytic bacteria in their gastrointestinal tracts. Therefore, the aim of this study was to isolate and identify cellulolytic bacteria from the feces of tropical endemic herbivores, including anoa (Bubalus depressicornis), banteng (Bos javanicus), muntjak (Muntiacus muntjak), and timor deer (Rusa timorensis). Bacteria were isolated using the serial dilution technique and screened on Carboxy Methyl Cellulose (CMC) media. The selected isolates were identified based on their morphological and biochemical characteristics, cellulase enzyme activity, and molecular identification of 16S rDNA. The result showed that a total of five bacterial isolates were isolated from feces of anoa, banteng, muntjak, and timor deer. In addition, isolates exhibited characteristics of facultative anaerobes with gram-positive coccus, fermenting glucose, fructose, sucrose, starch, and cellulose. Based on cellulolytic index, isolates from anoa and banteng feces showed high cellulolytic activity with an index of about 1.2, indicating their potential as cellulose-degrading bacteria. Molecular identification and phylogeny analysis of cellulolytic bacteria isolates from anoa and banteng feces showed 100% similarities with Enterococcus faecium. Therefore, bacteria from feces of tropical endemic herbivores, especially anoa and banteng, possess cellulolytic activity and have potential as cellulolytic probiotic for ruminants that feed on forage-based diet. This is the first study to document the cellulolytic activity of anoa, and banteng feces.
The addition of anaerobic fungi isolates from buffalo rumen to increase fiber digestibility, fermentation, and microbial population in ruminants SINTA AGUSTINA; KOMANG GEDE WIRYAWAN; SRI SUHARTI; ANJA MERYANDINI
Biodiversitas Journal of Biological Diversity Vol. 25 No. 1 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d250112

Abstract

Abstract. Agustina S, Wiryawan KG, Suharti S, Meryandini A. 2024. The addition of anaerobic fungi isolates from buffalo rumen to increase fiber digestibility, fermentation, and microbial population in ruminants. Biodiversitas 25: 107-115. Rumen microbes have an important role in the rumen. Anaerobic fungi are microbes needed in the forage digestion process in the rumen. The addition of microbes, particularly anaerobic fungi is essential to increase the digestibility of forage within rumen. Therefore, this study aimed to evaluate the addition of anaerobic fungi isolates from buffalo rumen to increase fiber digestibility, fermentation, and microbial population in sheep rumen. The in vitro tests were carried out using the Tilley and Terry method, using elephant grass and rice straw as tested forage. Piromyces sp. (F1, and F3), Caecomyces sp. (F2, and F5), and Neocallimastix frontalis (F4) isolates from buffalo rumen were used as tested anaerobic fungi. The result showed that the addition of anaerobic fungi isolates from buffalo rumen significantly affected fiber digestibility (Neutral Detergent Fiber (NDF), Acid Detergent Fiber (ADF), cellulose and hemicellulose) except lignin digestibility. The addition of N. frontalis had higher fiber digestibility which is 49.02% NDF digestibility, 42.11% ADF digestibility, 44.28% hemicellulose digestibility, and 38.60% cellulose digestibility. Furthermore, N. frontalis also significantly increased In vitro Dry Matter Digestibility (IVDMD), In vitro Organic Matter Digestibility (IVOMD), ammonium (NH3) production, total Volatile Fatty Acid (VFA) production, and microbial population compared to Piromyces sp., and Caecomyces sp. In conclusion, anaerobic fungus type N. frontalis showed promising potential to be used as a ruminant probiotic due to its superior effect on fiber digestibility, fermentation, and microbial population compared to Caecomyces sp. and Piromyces sp.
Co-Authors Anja Meryandini I Komang Gede Wiryawan KOMANG G WIRYAWAN KOMANG G. WIRYAWAN NUR NOVRARIANI NUR HIDAYAH RUKIAH LUBIS SINTA AGUSTINA SINTA AGUSTINA SINTA AGUSTINA