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All Journal JURNAL KIMIA SAINS DAN APLIKASI
Sri Fauziah
Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, Jalan Tanjung Kampus IPB Dramaga, Bogor 16680|IPB University|Indonesia
Chemical Engineering, Chemistry & Bioengineering Chemistry Engineering

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Thin Layer Chromatography Fingerprint Analysis of Tempuyung (Sonchus arvensis L.) Sri Fauziah; Puput Melati; Ade Heri Mulyati; Mohamad Rafi; Eti Rohaeti
Jurnal Kimia Sains dan Aplikasi Vol 26, No 5 (2023): Volume 26 Issue 5 Year 2023
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jksa.26.5.187-193

Abstract

Thin layer chromatography (TLC) fingerprint profile analysis can be used for quality control of herbal medicinal raw materials through identification, authentication, and discrimination. This study aims to develop a fingerprint analysis method for tempuyung TLC (Sonchus arvensis L.), which is then used for quality control. Tempuyung was extracted with methanol using ultrasonication which was then analyzed using the developed fingerprint TLC method. The optimum mobile phase used to separate compounds from tempuyung was a composition of chloroform: ethyl acetate: dichloromethane: formic acid (7.5:2:0.5:0.1) and produced eleven bands. The mobile phase composition produced good separation and had a typical luteolin band with an Rf value of 0.22, detected under UV 366 nm and derivatized with 10% sulfuric acid reagent. This method was applied to tempuyung from three locations, including Malang, Solo, and Yogyakarta, whose fingerprint patterns did not differ significantly. The fingerprint method has been validated and met the acceptance requirements for quality control of tempuyung.
Co-Authors Ade Heri Mulyati Eti Rohaeti Mohamad Rafi Puput Melati