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All Journal MDJ (Makassar Dental Journal)
Irene Edith Rieuwpassa, Irene Edith
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Dentistry

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Utilisation of collagen extract from tilapia fish skin waste (Oreochromis niloticus) for tissue regeneration: Pemanfaatan ekstrak kolagen dari limbah kulit ikan nila tilapia (Oreochromis niloticus) untuk regenerasi jaringan Putri, Nabilah Atikah; Ilhamsyah, Salsabyla Tamara Putri; Azzahra, Fatimah; Nusaly, Tiara Rezqi Megautama; Rieuwpassa, Irene Edith
Makassar Dental Journal Vol. 13 No. 3 (2024): Volume 13 Issue 3 Desember 2024
Publisher : Makassar Dental Journal PDGI Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35856/mdj.v13i3.1102

Abstract

Production and cultivation of tilapia fish (TF) (Oreochromis niloticus) in Indonesia is the second largest after China. However, TF waste is a potential pathogen. The waste of TF can be managed into valuable products, such as collagen extracts that can be used in dentistry in several dosage forms, such as sponges, hydrogels, membranes, and nano collagen fibres. There are various thera-peutic applications of collagen extracts from TF skin, especially in the field of dentistry, such as in the treatment of wounds heal-ing, dry socket healing, bone necrosis, grafting, flap surgery, and periodontal tissue regeneration. TF skin can be used as an ex-cellent and economical regenerative treatment to provide cost-effective treatment. However, larger-scale studies worldwide should be conducted before it is officially used widely.
The potential of Melaleuca cajuputi extract as an antibacterial, anti-iflammatory and osteogenic agent in oral regenerative therapy: Potensi ekstrak Melaleuca cajuputi sebagai agen antibakteri, antiinflamasi dan osteogenik dalam terapi regeneratif rongga mulut Rieuwpassa, Irene Edith; Hamka, Dinda Cindrahati; Izzah, Agnes Dea Ugie Wihdatul; Salamah, Ummi; Budiawan, Utami Putri; Anwar, Vina Maulydia; N, Nur Syabina Octavia Gamal; Zakiyah, A. Fadhilah Putri
Makassar Dental Journal Vol. 14 No. 2 (2025): Volume 14 Issue 2 August 2025
Publisher : Makassar Dental Journal PDGI Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35856/mdj.v14i2.1340

Abstract

Oral regenerative therapy requires agents with antibacterial and osteogenic properties to address hard tissue damage caused by infection or trauma. Galam leaves (Melaleuca cajuputi), a plant native to Southeast Asia, are known to contain bioactive com-pounds such as flavonoids, polyphenols, alkaloids, and saponins that support tissue healing. This article systematically reviews the potential of galam leaf extract as an antibacterial, anti-inflammatory, and osteogenic agent in oral regenerative therapy, based on literature evaluating the biological activity and safety of galam leaf extract, particularly against oral pathogenic bacteria and its ability to stimulate osteogenesis. Galam leaf extract has been proven to have antibacterial activity against S.mutans, Lactobacil-lus plantarum, and P.gingivalis. Its combination with tricalcium silicate cement increases the number of odontoblast-like cells and lymphocytes. Toxicity tests indicate that the extract is safe for topical use even at high concentrations. It is concluded that galam leaf extract is a candidate for the development of biomaterials in regenerative therapy due to its multifunctional and safe properties.
Extraction of keratinase enzyme from marine Actinomycetes and its antibacterial againts S.aureus : Ekstraksi enzim keratinase dari Actinomycetes laut dan potensi antibakterinya terhadap S.aureus Rieuwpassa, Irene Edith; Khatimah, Amraida Khusnul; Gunawan, Muh. Chaerul; Maulana, Lalu Novan; Makkawaru, Fadhlan Isnan; Sa, Salsabyila Wahyuni; Amin., Zalzabila M.
Makassar Dental Journal Vol. 14 No. 3 (2025): Volume 14 Issue 3 December 2025
Publisher : Makassar Dental Journal PDGI Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35856/mdj.v14i3.1333

Abstract

Staphylococcus aureus is an important pathogen often associated with serious infections and antibiotic resistance. In the search for alternative treatments, marine Actinomycetes are a potential source due to their ability to produce bioactive enzymes, one of which is keratinase. In addition to its function in keratin degradation, this enzyme is also known to have potential as an antibacte-rial agent. This article explores the extraction of keratinase enzymes from marine Actinomycetes isolates and tests their antibac-terial activity against S.aureus, as a first step in the utilisation of marine biotechnology for medical and pharmaceutical purposes. This systematic review was compiled based on the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Initial search results of 135 articles were then excluded, yielding four articles that showed that marine Ac-tinomycetes have antibacterial potential against S.aureus. It was concluded that the extraction of keratinase enzymes from ma-rine Actinomycetes showed significant antibacterial activity against S.aureus, indicating the potential of keratinase as an alter-native agent for treating gram-positive bacterial infections, especially amid increasing resistance to antibiotics.
Co-Authors Amin., Zalzabila M. Anwar, Vina Maulydia Budiawan, Utami Putri Fatimah Azzahra Gunawan, Muh. Chaerul Hamka, Dinda Cindrahati Ilhamsyah, Salsabyla Tamara Putri Izzah, Agnes Dea Ugie Wihdatul Khatimah, Amraida Khusnul Makkawaru, Fadhlan Isnan Maulana, Lalu Novan N, Nur Syabina Octavia Gamal Nusaly, Tiara Rezqi Megautama Putri, Nabilah Atikah Sa, Salsabyila Wahyuni Salamah, Ummi Zakiyah, A. Fadhilah Putri