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Rika Wahyuningtyas, Rika
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Engineering Environmental Science

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THE EFFECT OF CRUDE PROTEIN HALIMEDA SP. ON CYPRINUS CARPIO INFECTED KOI HERPES VIRUS ON EXPRESSION OF MAJOR HISTOCOMPATIBILITY COMPLEX CLASS-1 Yanuhar, Uun; Wahyuningtyas, Rika
JFMR (Journal of Fisheries and Marine Research) Vol. 1 No. 1 (2017): JFMR
Publisher : Faculty of Fisheries and Marine Science, Brawijaya University, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfmr.2017.001.01.3

Abstract

Halimeda sp. is a kind species of macroalgae that abundant grow in Indonesia. The utilization of Halimeda especially protein for disease prevention caused by the virus has not been done to the fish. The purpose of the study is to know the  treatment of crude protein Halimeda sp on  Cyprinus carpio infected by Koi Harvest Virus (KHV) on the expression of Major Histocompatibility Complex (MHC) class 1. MHC class-1 is one of the immune adaptive response on fish like this C. carpio. The methods are isolation of crude protein Halimeda sp,   purification  of  crude protein, Haemagglutination (HA) test and dot blot test. The result shows that the crude protein of Halimeda sp. can produce of adaptive immune response like MHC class 1. MHC class-1 has the function of  the immune system for maintenance of virus attacks directly. Responsible of treatment crude protein Halimeda sp. on C. carpio can be showed quantitatively using the dot blot test result. The  conclusion  is the crude protein of Halimeda sp. able to activate the MHC class I and to inhibit the proliferation of the KHV and maintenance of the fish cell of C. carpio..
Co-Authors Yanuhar, Uun