I Wayan Suardana
Laboratory of Veterinary Public Health, Faculty of Veterinary Medicine Udayana University, Jl. PB. Sudirman Denpasar, Bali, Indonesia 80225.

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Detection Of Antibiotic Residues In Chicken Eggs At The Chicken Egg Farmers And Egg Distributors On Trading Business In Denpasar Municipality Maria Clafita Witoko; I Wayan Suardana; Mas Djok Rudyanto
Journal of Veterinary and Animal Sciences Vol 2 No 2 (2019)
Publisher : Institute for Research and Community Service, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JVAS.2019.v02.i02.p05

Abstract

Antibiotic residues often found in food animal due to antibiotic usage for prevention and medication of diseases in livestock. The usage of antibiotics as growth promoter also often done by farmers to increase feed efficiency and enhance livestock’s growth, producing larger and heavier livestock that are more profitable. Food animal that contains antibiotic residues are neither safe nor qualified as human consumption. The objective of this research was to detect antibiotic residues in chicken eggs at chicken egg farmers and egg distributors in Denpasar, Bali. In order to determine antibiotic residues, a total of 24 chicken egg samples were randomly collected from farmers and distributors, followed by testing with bioassay method. The result showed that 13 out of 18 samples collected from farmers and 1 out of 6 samples collected from distributors were tested positive for antibiotic residues. Most of the tested positive samples contained aminoglycosides residue (66.7% on eggs collected from farmers), followed by tetracycline residue (44,4 on farmers egg and 16,7% on distributors egg) and macrolide residue (16,7% on farmers egg).
Isolated Hemolysis Profile of Streptococcus Sp. Isolation Result from Swine’s Tonsil In Slaughter House at Punggul and Bongkasa Village Carene Naomi; I Wayan Suardana; I Nyoman Suarsana
Journal of Veterinary and Animal Sciences Vol 2 No 2 (2019)
Publisher : Institute for Research and Community Service, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JVAS.2019.v02.i02.p01

Abstract

Streptococcus is a Gram-positive coccus from the family Streptococcaceae that can be found normally in some healthy animal but can cause a disease as well. The purpose of this research is to know the hemolysis profile from pig’s tonsil swab isolated from the traditional pig slaughterhouse at Punggul Village and Bongkasa Village that can be divided into three hemolytic profile; alpha-hemolytic, beta hemolytic, and gamma hemolytic, to know the pathogenicity. This research is divided into several steps, such as; isolating the swab, Gram test, catalase test, oxidase test, and hemolysis test on Sheep Blood Agar. Streptococcus is a Gram-positive coccus, tested Gram positive, catalase negative and oxidase positive. This research concludes the amount of positive Streptococcus sp sample based on hemolysis profile is 6 of 24 samples, whereas 3 of 24 samples (12,5%) has ? hemolysis pattern, 3 of 24 samples (12,5%) has ? hemolysis pattern and none showed the ? pattern. The conclusion from the table; showed positive sample of Streptococcus sp is divided 50:50 for the ? and ? hemolysis, and no sample showed the ? pattern. At most cases, the ? and ? hemolysis Streptococcus is a pathogen bacterium. This data might be useful for references to see the transmission of Streptococcus sp in swine at Abiansemal area in Badung, Bali
Hemolytical Profile Of Streptococcus Spfrom Nasal Swab Isolation At Traditional Farm In Bongkasa Village, Abiansemal Subdistrict, Badung Regency, Bali. Ni Luh Made Siska Yanti; I Wayan Suardana; I Gusti Ketut Suarjana
Journal of Veterinary and Animal Sciences Vol 2 No 2 (2019)
Publisher : Institute for Research and Community Service, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JVAS.2019.v02.i02.p02

Abstract

Streptococcus sp is Gram positive cocci and aligned like chain. This bacteria can be found normally in upper respiratory tract. Streptococcus sp can be divided into two groups; able to hemolize blood and unable. B-hemolytic Streptococcus tend to be more pathogen than the others. Therefore this research about Streptococcus sp’s hemolysis profile is very important. The samples of this research is an isolated swine nasal swab from a traditional pig farm in Bongkasa Village. 20 samples were used in this research. Samples were grown in Blood Agar media to find out the capability of bacteria for hemolizing blood. Catalase test used for differentiating Streptococcus sp and Staphylococcus sp, followed by oxydase test after. Result of the research shows positive samples of Streptococcus sp divided by hemolysis profile is 11 isolates whereas 36,4% isolates were Alfa hemolysis, 54,6% were B hemolysis, and 9% isolates were Gamma hemolysis.