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I Wayan Suardana
Faculty of Veterinary Medicine Udayana University
Veterinary

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Studies On Species Of Lactic Acid Bacteria Isolate Sr 12 From Bali Cattle Gastric With Conventional Method And Api Kit 50ch Made Radwika; I Wayan Suardana; I Dewa Made Sukrama
Journal of Veterinary and Animal Sciences Vol 2 No 1 (2019)
Publisher : Institute for Research and Community Service, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JVAS.2019.v02.i01.p03

Abstract

Lactic acid bacteria (LAB) are Gram positive bacteria that characterized by non-motil, non-spore, negative catalase, coccus or bacillus cell shape, and live in microaerophilic condition with CO­2 aeration 5-7%. The purpose of study was to identifyy LAB isolate SR 12 isolated from gastrointestinal tract of bali cattle that previously known have potency as a source of probiotic. The study initiated by cultivation of isolate on de Man, Rogosa, Sharpe (MRS) medium, confirmation of isolate with Gram staining and catalase test as a main procedure for Gram positive indentification. The next identification in order to know the species of isolate was conducted both conventional test and kit API 50CH test. The result of study showed the LAB SR12 isolate as a genus of Pediococcusor Enterococcus according to its growth in temperature 10oC, pH9,6 and it can’t grow in halophilic condition. On the other hands, the use of ofAPI kit 50CH test showed the LAB SR12 was detected as Pediococcus pentosaceus.
Probiotic Potential Test for Lactic Acid Bacterial Isolate from Labi Cattle Gastric Again Low Acidity and Sodium Deoxycholate I Made Rahma Sanjaya; I Wayan Suardana; I Nyoman Suarsana
Journal of Veterinary and Animal Sciences Vol 2 No 1 (2019)
Publisher : Institute for Research and Community Service, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JVAS.2019.v02.i01.p04

Abstract

Lactic acid bacteria (LAB) are one of group probiotic bacteria, which have a positive impact on human and animal health. The aim of this research was to determine the potency lactic acid bacterial isolate from LABi cattle gastric as a probiotic that have resistance to low pH and sodium deoxycholate. The study was started with the cultivation test that aims to made sure uncontaminated isolates of microorganisms, including test for growth response in MRS broth medium, test on catalase production, and Gram stain. Its potency for probiotic development was tested by growing of isolate in MRS broth medium with low pH conditions (pH 2, 3, 4) and in MRS medium supplemented with various concentration of sodium deoxycholate (0,2 mM, 0,4 mM, 0,6 mM). The result showed that isolate from LABi cattle gastric was able to grow well in MRS broth medium under anaerobic condition. In addition, it also showed catalase negative and Gram-positive bacteria. In the main experiment, this isolate was found to be resistant to low pH conditions (up to pH 2) and high concentration of sodium deoxycholate (up to 0,6 mM). These results indicate that the isolate the lactic acid bacteria have resistance to low pH and the sodium deoxycholate, which is the main requirement of the probiotic bacteria
Studies on Lactic Acid Bacteria Isolate Sr 13 From Bali Cattle Gastric Bagus Nanda Govinda Muria Siddhi; I Wayan Suardana; Nyoman Semadi Antara
Journal of Veterinary and Animal Sciences Vol 2 No 1 (2019)
Publisher : Institute for Research and Community Service, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JVAS.2019.v02.i01.p05

Abstract

Lactic acid bacteria (LAB) is a generic term for bacteria that ferment lactose and produce lactic acid as its main product. In general, lactic acid bacteria belonging to Gram positive bacteria, negative catalase, does not form spores, have no cytochrome, aerotolerant, anaerobic to microaerophilic, require complex nutrients such as amino acids, vitamins (B1, B6, B12 and biotin), purine, pyrimidine. This study aims to determine the species of lactic acid bacteria (LAB) from isolate SR13 isolated from rumen liquid of bali cattle. The identification was started by cultivation of isolate on de Man, Rogosa, Sharpe (MRS) medium with anaerobic condition, confirmation of isolates by Gram staining and catalase test, and finally identificion of species by using conventional test and 50CH API test kit. The results of study showed, the isolate of LAB SR 13 was detected asPediococcus/Enterococcus, moreover, identification by using 50CH Api test kit showed as Pediococcus pentosaceus 2
The Growth Hormone Level of Bali Cattle’s Post Treatment with Ethinil Esthradiol and Progesteron Hormones in Combination with Mineral Aletha Yuliana Mandala; Ni Ketut Suwiti; I Wayan Suardana
Journal of Veterinary and Animal Sciences Vol 2 No 1 (2019)
Publisher : Institute for Research and Community Service, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JVAS.2019.v02.i01.p01

Abstract

This study aims to find out the growth hormone level of bali cattle’s post giving of ethinyl estradiol and progesterone hormones combined with mineral. The total sample of 64 bali cattle is divided into 4: Group I (Control) is not given hormone ethinyl estradiol, progesterone and mineral. Group II is given the hormone ethinyl estradiol and progesterone, without mineral. Group III was not given ethinyl estradiol and progesterone hormones, with mineral administration. Group IV is given hormone ethinyl estradiol, progesterone and mineral. At the end of the study, measurements were performed to determine the growth hormone level using ELISA method. The data of the research were analyzed by the analysis of variance. The result showed that male cattle growth hormone (268.281±73.13pg/ml) was higher than females (236.250±13.79pg/ml). The growth hormone level of young cattle was higher (264.94±74.42pg/ml) than adult (239.59±14.05pg/ml). Level of growth hormone of bali cattle with highest minerals(266.97±74.15pg/ml) compared without minerals (237.56±11.05pg/ml). The growth hormone level of bali cattle with higher levels of ethinyl estradiol and progesterone hormone (263.31±74.81pg/ml) compared with no ethinyl estradiol and progesterone hormone (241.22±18.8pg/ml). The results are age and sex effect on growth hormone level with hormone ethinyl estradiol and progesterone combined with mineral. Giving ethinyl estradiol and progesterone hormones combined with significant mineral (P<0.05) may increase the growth hormone level of young male bali cattle.
Co-Authors Aletha Yuliana Mandala Bagus Nanda Govinda Muria Siddhi I Dewa Made Sukrama I Made Rahma Sanjaya I Nyoman Suarsana Made Radwika Ni Ketut Suwiti Nyoman Semadi Antara