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I Gede Putu Wirawan
Faculty of Agriculture, Udayana University, Jl. PB. Sudirman, Denpasar, Bali 80852, Indonesia
Biochemistry, Genetics & Molecular Biology Environmental Science

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Identification of Phytochemical Compounds of Ethyl Acetate Extract of Bulung Anggur (Caulerpa Sp.) By Gc-Ms And Toxicity Test On Artemia Salina Leach Shrimp Larvae Ester Giwo; Nguyen Ti Ha; I Gede Putu Wirawan; I Nyoman Wijaya; I Ketut Suada
International Journal of Biosciences and Biotechnology Vol 10 No 2 (2023): INTERNATIONAL JOURNAL OF BIOSCIENCES AND BIOTECHNOLOGY
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2023.v10.i02.p05

Abstract

Sea grape (Caulerpa sp.) is one of the potential types of seaweed. Sea grape potential as a natural ingredient for making medicine. Balinese people use sea grape for consumption. The content of bioactive compounds in sea grape is suspected to be toxic at certain doses. The purpose of this study was to determine the compound content of ethyl acetate extract of sea grape (Caulerpa sp.) and the toxicity on Artemia salina Leach. The identification of the bioactive compounds was carried out by Gas Chromatography-Mass Spectrophotometry and the toxicity test of extracts was carried out using the Brine Shrimp Lethality Test (BSLT) method. The results showed that the ethyl acetate extract of sea grape (Caulerpa sp.) contained 27 chemical compounds, 7 of which had a quality value > 70%, namely Propanoic acid, ethyl ester; n-Propyl acetate; sec-Butyl acetate; Toluene; Acetic acid, butyl ester; Bicyclo [4.2.0] octa-1, 3, 5-triene; and Styrene. The results of the toxicity test of Artemia salina Leach are toxic which had an LC50 value of 44,070 ppm so that they can be used as a larvicide
Phytochemical Analysis of Bulung Boni (Caulerpa cylindracea S.) N- Hexane Extract with Gc-Ms Method and Toxicity Test on Mice (Mus musculus L.) May Disa Br Silalahi; I Gede Putu Wirawan; I Nyoman Wijaya; I Ketut Suada; Trisna Agung Phabiola; Angelika Astaykina
International Journal of Biosciences and Biotechnology Vol 10 No 2 (2023): INTERNATIONAL JOURNAL OF BIOSCIENCES AND BIOTECHNOLOGY
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2023.v10.i02.p06

Abstract

One of the marine resource commodities owned by Indonesia is seaweed. Bulung boni is one type of seaweed that grown in Indonesia. Bulung boni is the regional name of Caulerpa cylindracea. Bulung boni is usually used as a food and medicine for most people in Bali. The purpose of this research is to study the content of phytochemical compounds in n-hexane extract of bulung boni and determine the toxicity effect on mice (Mus musculus). The methods used in this study were maceration, GC-MS (Gas Chromatography-Mass Spectrometry), observation, and calculation with the Thomson and Weil formula. This study used 24 mice as experimental animals. Mice were divided into 6 treatment groups, group 1 as control was given 1% Na-CMC solution, groups 2-6 were given test extracts with doses, 100, 200, 300, 400, 500 mg/30 g BW mice. The results showed the highest compound contained in the n-hexane extract of bulung boni was Hexadecanoic acid, methyl ester with an AUC value of 35.21%. Toxicity tests on mice showed no deaths experienced by mice, so the LD50 value determined was pseudo at 16.6 g/ kg BW and fell into the practically non-toxic category
Co-Authors Angelika Astaykina Ester Giwo I Ketut Suada I Nyoman Wijaya May Disa Br Silalahi Nguyen Ti Ha Trisna Agung Phabiola