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Judiono ., Judiono
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EFFECTS OF ORAL CLEAR KEFIR PROBIOTICS ON GLYCEMIC STATUS, LIPID PEROXIDATION, ANTIOXIDATIVE PROPERTIES OF STREPTOZOTOCIN INDUCED HYPERGLYCEMIA WISTAR RATS ., Judiono; ., Djokomoeljanto; S, Hadisaputro
GIZI INDONESIA Vol 34, No 1 (2011): Maret 2011
Publisher : PERSATUAN AHLI GIZI INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36457/gizindo.v34i1.95

Abstract

Hyperglycemia causes excessive free radicals, may increase reactive oxygen species, lipid peroxidation, and reduced antioxidants as well as dysfuntion of the pancreatic β cells. This study was aiming to investigate the effect of oral Clear kefir probiotic on glycemic status, lipid peroxidation and antioxidantive properties of Streptozotocin induced hyperglycemia Wistar Rats. The randomized pretest - posttest control group study design was conducted in 84 malehyperglycemia Wistar rats induced by 40 mg / kg bw streptozotocin (STZ) dissolved in 0,1 M buffer citrate pH 4,5. Rats were randomized into four groups, namely: (1) STZ-induced animals group and given insulin treatment UI/200 0.76 g bw, (2) STZ-induced animals group and given treatment clear kefir 3.6 cc/200 g bw/day for 30 days, (3) STZ-induced animalsgroup as a positive control (ad libitum), (4) normal animals group as a negative control (ad libitum).Blood glucose was measured by enzymatic method. Lipid peroxidation measured of MDA-TBARs by spectrophotometry. SOD and GPX Antioxidants were measured by ELISA. Catalase was measured by spectrophotometry. Probiotics Clear kefir characterization was done by microbiology identification. Data were analyzed by One Way Anova, Kruskall Walis, Duncan, Mann Whitneytest with significance level p 0.05. The result showed that clear kefir supplementation 3.6 cc / day for 30 days administration, affected on blood glucose, MDA and increased antioxidant capacity. Statistical analysis showed that there were respectively decreased of glucose (p0.001), MDA (p0.001). SOD antioxidant capacity was increased (0,05), in addition GPx and Cat were also inceased(p0,001), except in control groups. Probiotics kefir was found as many as 10(6)-10(9) cfu / mL and declined to 10(5) as the decrease in pH during storage, four species of probiotics were detected, such as: Lactobacillus Sp, Sp Lactococcus and Acetobacter and Saccharomyces Sp.In conclusion, kefir supplementation significantlydecreased the blood glucose level, level of MDA and increased of antioxidants capacity. The number of probiotics declined during storage.It is interesting to identify a potential clear kefir probiotics in a pathogenesis of the β cells pancreatic repair and stability product during storage for future study. Keywords: probiotic, hyperglycemia, free radicals, lipid peroxidation, antioxidant
STUDI EFFEKTIVITAS KEFIR BENING DAN MENIRAN (Philanthus niruri) TERHADAP PENURUNAN KADAR GLUKOSA DARAH PADA HEWAN TIKUS WISTAR HIPERGLIKEMIA DIINDUKSI STREPTOZOTOCIN ., Judiono; Hadisaputro, Soeharyo; Djokomoeljanto, RRJ; Laksono, Budi; W, Theophilus
GIZI INDONESIA Vol 33, No 2 (2010): September 2010
Publisher : PERSATUAN AHLI GIZI INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36457/gizindo.v33i2.89

Abstract

EFFECTIVITY STUDY OF PLAIN KEFIR AND MENIRAN (PHILANTUS NIRURI) ON BLOOD GLUCOSE LEVELS ON HYPERGLYCEMIA WISTAR RATS INDUCED STREPTOZOTOCINTraditional medicines and functional foods used for diabetes therapy increased in Indonesia. Bioactive components play an important role in decreasing blood glucose. The study aims to prove the effectiveness of plain kefir and Meniran (Philanthus niruri) to decrease blood glucose levels in wistar rats with streptozotocin-induced hyperglycemia. The study design was randomized pre – post test control group. A number of 60 male rats aged 2.5-3 months, 150-250 g were made hyperglycemia by induced streptozotocin (STZ) 40 mg / kg berat badan. The hyperglycemic rats were grouped into: (1) treated with 0.76 UI insulin /day, (2) treated with 3.6 mL plain kefir /day, (3) treated 2.7 mL meniran / day, (4) positive control group of hyperglycemic rats, and (5) negative control group (standard diet ad libitum). The ratswere given feed refers to the AIN 93. Fasting glucose levels before and after treatment were measured by Super (Glucocard II) enzimatic method. The results showed that body weight increased in all treatments, except meniran, however the increaments were not differ significantly. Changes in body weight of insulin group were 13.800 + 16.104 g, kefir treatment amounted to 13.812 + 21.294, and meniran increased about 18.394 + 16.225. Changes in blood glucose in the insulin group about -118.571+55.815 mg/dL, kefir treatment at -102.875+60.454, while meniran group -66,625+37,784. In contrast, the positive control group tended to increase glucose levels. Conclusion In vitro, plain kefir and meniran potentially lowered blood glucose levels. However, kefir showed lowered glucose level better than meniran. Futher research needs to study bio- molecular mechanisms of the decline in the future.Key words: diabetes mellitus, meniran, plain kefir, streptozotocin (STZ), hyperglycemic rat
STUDI EFFEKTIVITAS KEFIR BENING DAN MENIRAN (Philanthus niruri) TERHADAP PENURUNAN KADAR GLUKOSA DARAH PADA HEWAN TIKUS WISTAR HIPERGLIKEMIA DIINDUKSI STREPTOZOTOCIN ., Judiono; Hadisaputro, Soeharyo; Djokomoeljanto, RRJ; Laksono, Budi; W, Theophilus
GIZI INDONESIA Vol 33, No 2 (2010): September 2010
Publisher : PERSATUAN AHLI GIZI INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (523.919 KB)

Abstract

EFFECTIVITY STUDY OF PLAIN KEFIR AND MENIRAN (PHILANTUS NIRURI) ON BLOOD GLUCOSE LEVELS ON HYPERGLYCEMIA WISTAR RATS INDUCED STREPTOZOTOCINTraditional medicines and functional foods used for diabetes therapy increased in Indonesia. Bioactive components play an important role in decreasing blood glucose. The study aims to prove the effectiveness of plain kefir and Meniran (Philanthus niruri) to decrease blood glucose levels in wistar rats with streptozotocin-induced hyperglycemia. The study design was randomized pre – post test control group. A number of 60 male rats aged 2.5-3 months, 150-250 g were made hyperglycemia by induced streptozotocin (STZ) 40 mg / kg berat badan. The hyperglycemic rats were grouped into: (1) treated with 0.76 UI insulin /day, (2) treated with 3.6 mL plain kefir /day, (3) treated 2.7 mL meniran / day, (4) positive control group of hyperglycemic rats, and (5) negative control group (standard diet ad libitum). The ratswere given feed refers to the AIN 93. Fasting glucose levels before and after treatment were measured by Super (Glucocard II) enzimatic method. The results showed that body weight increased in all treatments, except meniran, however the increaments were not differ significantly. Changes in body weight of insulin group were 13.800 + 16.104 g, kefir treatment amounted to 13.812 + 21.294, and meniran increased about 18.394 + 16.225. Changes in blood glucose in the insulin group about -118.571+55.815 mg/dL, kefir treatment at -102.875+60.454, while meniran group -66,625+37,784. In contrast, the positive control group tended to increase glucose levels. Conclusion In vitro, plain kefir and meniran potentially lowered blood glucose levels. However, kefir showed lowered glucose level better than meniran. Futher research needs to study bio- molecular mechanisms of the decline in the future.Key words: diabetes mellitus, meniran, plain kefir, streptozotocin (STZ), hyperglycemic rat
EFFECTS OF ORAL CLEAR KEFIR PROBIOTICS ON GLYCEMIC STATUS, LIPID PEROXIDATION, ANTIOXIDATIVE PROPERTIES OF STREPTOZOTOCIN INDUCED HYPERGLYCEMIA WISTAR RATS ., Judiono; ., Djokomoeljanto; S, Hadisaputro
GIZI INDONESIA Vol 34, No 1 (2011): Maret 2011
Publisher : PERSATUAN AHLI GIZI INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (129.181 KB)

Abstract

Hyperglycemia causes excessive free radicals, may increase reactive oxygen species, lipid peroxidation, and reduced antioxidants as well as dysfuntion of the pancreatic β cells. This study was aiming to investigate the effect of oral Clear kefir probiotic on glycemic status, lipid peroxidation and antioxidantive properties of Streptozotocin induced hyperglycemia Wistar Rats. The randomized pretest - posttest control group study design was conducted in 84 malehyperglycemia Wistar rats induced by 40 mg / kg bw streptozotocin (STZ) dissolved in 0,1 M buffer citrate pH 4,5. Rats were randomized into four groups, namely: (1) STZ-induced animals group and given insulin treatment UI/200 0.76 g bw, (2) STZ-induced animals group and given treatment clear kefir 3.6 cc/200 g bw/day for 30 days, (3) STZ-induced animalsgroup as a positive control (ad libitum), (4) normal animals group as a negative control (ad libitum).Blood glucose was measured by enzymatic method. Lipid peroxidation measured of MDA-TBARs by spectrophotometry. SOD and GPX Antioxidants were measured by ELISA. Catalase was measured by spectrophotometry. Probiotics Clear kefir characterization was done by microbiology identification. Data were analyzed by One Way Anova, Kruskall Walis, Duncan, Mann Whitneytest with significance level p <0.05. The result showed that clear kefir supplementation 3.6 cc / day for 30 days administration, affected on blood glucose, MDA and increased antioxidant capacity. Statistical analysis showed that there were respectively decreased of glucose (p<0.001), MDA (p<0.001). SOD antioxidant capacity was increased (<0,05), in addition GPx and Cat were also inceased(p<0,001), except in control groups. Probiotics kefir was found as many as 10(6)-10(9) cfu / mL and declined to 10(5) as the decrease in pH during storage, four species of probiotics were detected, such as: Lactobacillus Sp, Sp Lactococcus and Acetobacter and Saccharomyces Sp.In conclusion, kefir supplementation significantlydecreased the blood glucose level, level of MDA and increased of antioxidants capacity. The number of probiotics declined during storage.It is interesting to identify a potential clear kefir probiotics in a pathogenesis of the β cells pancreatic repair and stability product during storage for future study. Keywords: probiotic, hyperglycemia, free radicals, lipid peroxidation, antioxidant
Co-Authors Budi Laksono Djokomoeljanto ., Djokomoeljanto Hadisaputro S, Hadisaputro RRJ Djokomoeljanto, RRJ Soeharyo Hadisaputro Theophilus W, Theophilus