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Herlina Rante
Laboratorium Mikrobiologi Fakultas Farmasi Universitas Hasanuddin
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Antibacterial Activity of Ethanol Extract of Arabian Lotus Leaves (Ziziphus spina-christi L.) and Bioautography TLC Analysis of Several Pathogenic Bacteria Herlina Rante; Marni Pabisa; Burhanuddin Taebe
bionature Vol 24, No 2 (2023): Oktober
Publisher : Fakultas MIPA UNM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/bionature.v24i2.52766

Abstract

Bidara arab leaves (Ziziphus spina-christi L), contain various potentially health-beneficial compounds, including antibacterial agents. The aim of this study was to determine the antibacterial activity of ethanolic leaf extract of Ziziphus spina-christi L. in inhibiting the growth of pathogenic bacteria. The leaves of Bidara arab were macerated using 70% ethanol, followed by the agar diffusion method to evaluate the antibacterial activity. Subsequently, bioautography analysis using TLC and phytochemical screening of the active extract were conducted. The results of the antibacterial activity test using the ethanolic leaf extract of Ziziphus spina-christi L. showed antibacterial activity at a concentration of 2 mg/paper disk, with inhibition zone diameters of 19.44 mm for Bacillus subtillis, 17.06 mm for Escherichia coli, 16.32 mm for Staphylococcus aureus, 12.96 mm for Pseudomonas aeruginosa, and 14.50 mm for Salmonella typhi. The TLC-bioautography analysis of the ethanolic leaf extract of Ziziphus spina-christi L. against Bacillus subtillis exhibited inhibition at Rf 0.96, Escherichia coli at Rf 0.31, Staphylococcus aureus at Rf 0.89, while Salmonella typhi did not show inhibitory activity. Phytochemical screening results indicated that the ethanolic leaf extract of Bidara arab is suspected to contain flavonoids, terpenoids, and tannins.
Antibacterial Activity of Ethanol Extract of Arabian Bidara Leaves (Ziziphus spina-christi L) and Bioautography TLC Analysis Against Pathogenic Bacteria Herlina Rante; Marni Pabisa; Burhanuddin Taebe
Bionature Vol. 24 No. 2 (2023): Oktober 2023
Publisher : Jurusan Biologi Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The Ziziphus spina-christi L. plant, commonly known as Arabian bidara, are rich in potentially health-beneficial compounds, particularly those with antibacterial properties. This research aimed to investigate the effectiveness of an ethanolic leaf extract in inhibiting the growth of pathogenic bacteria. The leaves were subjected to maceration using 70% ethanol, followed by an assessment of their antibacterial properties using the agar diffusion method. Subsequently, the active extract was analyzed through bioautography using thin-layer chromatography (TLC), and its phytochemical spotted was screened. The results of the antibacterial activity using the ethanolic leaf extract revealed that it exhibited antibacterial activity when applied at a concentration of 2 mg per paper disk. Subsequently, it showed inhibition zones with diameters of 19.44 mm for Bacillus subtillis; Escherichia coli (17.06 mm); Staphylococcus aureus (16.32 mm); Pseudomonas aeruginosa (12.96 mm), and Salmonella typhi (14.50 mm). The TLC-bioautography analysis of the ethanolic leaf extract demonstrated inhibitory activity against Bacillus subtillis of Rf value with 0.96, Escherichia coli (0.31) and Staphylococcus aureus (0.89). However, no inhibitory activity was observed against Salmonella typhi. The phytochemical screening results suggested the presence of flavonoids, terpenoids, and tannins in the ethanolic leaf extract.
Co-Authors Burhanuddin Taebe Marni Pabisa