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All Journal Animal Production : Indonesian Journal of Animal Production
SD Rasad, SD
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Veterinary

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The Effect of Caffeine Supplementation in Egg-Yolk Tris Extender on Motility and Velocity of Garut Sheep Sperm Separation Rasad, SD; Soeparna, Soeparna; Nurfaidah, L
ANIMAL PRODUCTION Vol 12, No 3 (2010): September
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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The aims of this research were to know the effect of caffeine in Tris egg yolk extender on motility and velocity of Garut Ram sperm after separation and to find out the optimum dosage of caffeine for both parameters. This research used experimental design with Completely Randomized Design (CRD) with four treatments on different dosage  (0 mM, 2 mM, 4 mM and 6 mM), each treatment is repeated five times. The result showed that the supplemtation of caffeine significantly influence sperm motility  of Garut Ram sperm after separation. The highest sperm motility was on dosage 4 mM that was 63.57%  on top fraction and 65.90% on below fraction. The highest sperm velocity was on dosage 4 mM that was 62.37 µm/second on top fraction and 64.24 µm/second on below fraction and significantly influence sperm velocity. The conclusion, 4 mM dosage of caffeine  was the optimum dosage to reach the highest motility and velocity of Garut Ram sperm both on top  and below fractions. (Animal Production 12(3): 139-143 (2010)Key Words: spermatozoa, Garut ram, sperm separation, caffeine
Effects of GnRH and PGF2 Injection on Progesterone Profile of Postpartum Dairy Cow Rasad, SD
ANIMAL PRODUCTION Vol 10, No 1 (2008): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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A research of progesterone profile on postpartum dairy cows after GnRH and PGF2a treatment had been conducted to determine the skim milk progesterone profiles, which enhance ovarian activity in postpartum dairy cows.  It is expected that by this treatment, the interval to first estrus could be reduced, and frequency of estrus and percentage of estrus expression could be increased. Twenty cows were randomly assigned to four treatment groups including Control group, which contain respectively 5 cows each group.  Cow (n=5) in group I received 200 mg GnRH i.m. on days 10-14 p.p.; in group II received 15 mg PGF2a on days 20-24 i.m. p.p.; and group III received combination of both.  The control group did not receive hormonal treatments.  The research used multivariate, completely randomized design, univariate F-test and Duncan’s Multiple Range Test. Results showed that the postpartum interval to first estrus was shorter in treated groups (I, 26 d; II, 22 d, and III, 24 d) as compared to control group (54 d, P< 0.05).  The percentage of estrus increased (60%) in group II and III, but no significant differences were found between group I and the control group (40%). Our result suggested that the combination treatment of GnRH and PGF2a (GnRH + PGF2α) enhanced activity in the postpartum cows, resulting in improved reproductive performance. (Animal Production 10(1): 16-21 (2008) Key Words: GnRH, PGF2a , progesterone profile, postpartum dairy cow
Quality of Cauda Epididymal Spermatozoa of Ongole Crossbred Bull in Skim Milk, Tris, and Citrate Extenders Added with Egg Yolk Solihati, N; Idi, R; Rasad, SD; Rizal, M; Fitriati, M
ANIMAL PRODUCTION Vol 10, No 1 (2008): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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The objective of this study was to find out the quality of cauda epididymal spermatozoa in skim milk, Tris, and citrate extenders added with egg yolk stored at  4-5oC.  The study was laid out in a Completely Randomized Design (CRD) with three treatments and six replications. Evaluation of the quality of fresh spermatozoa was done after diluted with different extenders and stored at 4-5oC. The results showed that extenders have no significant effects (P>0.05) on motility, live sperms, abnormality, intact plasma membrane and viability of cauda epididymal spermatozoa stored at 4-5oC.  It can be concluded that skim milk, citrate, and Tris extenders added with egg yolk have the same ability in maintaining the quality and viability of cauda epididymal sperms of Ongole crossbred bulls. (Animal Production 10(1): 22-29 (2008) Key Words: Ongol crossbred, spermatozoa, cauda epididymal, extenders
The Effect of Fructose Addition in Semen Extender on Quality of Separation of Garut Ram Sperm in Several Storage Length Rasad, SD; Simanjuntak, LC
ANIMAL PRODUCTION Vol 11, No 3 (2009): September
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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The objective of the research were to find out the effect of fructose addition in semen extender to the quality of separation Garut ram sperm and their length viability (up to 40% motility) in cool storage. Research design was split plot with four treatments of fructose addition in coconut water extender as a main plot i.e.: 1% (P1); 1.25% (P2); 1.50 (P3) and 1.75% (P4) and the length of sperm viability in cool storage (0; 3rd ; 6 th;9 th;12 th and 15 th hour), as a subplot. Each treatment was repeated five times. Sperm separation was done by albumin column method with 10 % concentration in top layer and 30%  in lower layer. Parameters of research were sperm motility and abnormality  after separation. Research result showed that addition of 1.75%  fructose  has the best sperm motility of  57.18± 1.43% and abnormality of 5.76±0.20% in 12 hours cool storage (top layer of albumin column). Sperm motility of 57.62 ± 0.65 % and abnormality of 4.35 ± 0.25% in 12 hours cool storage (lower layer of albumin column). As a conclusion, addition of fructose in coconut water semen extender has positive influenced on sperm quality. (Animal Production 11(3): 196-201 (2009)Key Words: fructose, sperm quality, extender, Garut ram
Co-Authors L Nurfaidah, L LC Simanjuntak, LC M Fitriati M Rizal N Solihati, N R Idi, R Soeparna Soeparna, Soeparna