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All Journal Animal Production : Indonesian Journal of Animal Production
P Summers, P
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Veterinary

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In Vitro Motility, Velocity and Capacitation Status of Merino Ram Spermatozoa Ismaya, Ismaya; Fiztpatrick, L; Summers, P
ANIMAL PRODUCTION Vol 14, No 2 (2012): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

The objective of the present study was to determine the effects of Merino rams on the in vitro motility, velocity and capacitation status of ram spermatozoa. Four rams of proven fertility were used in this study. Semen was collected by electro ejaculation. The fresh semen was diluted at four dilutions in Hepes buffered synthetic oviduct fluid. A sample of semen was collected and the motility, velocity and capacitation status of spermatozoa determined and analyzed. The results study showed that motility and velocity of Merino rams spermatozoa were not significantly different. The mean average path velocity (VAP), straight-line velocity (VSL) and curvilinear velocity (VCL) were 91.2 µm/s, 72.2 µm/s and 134 µm/s, respectively. Amplitude of lateral head displacement (ALH), beat cross frequency (BCF) and linearity (LIN) was 5.5 µm, 19.5 Hz, and 54.7%, respectively. During the incubation time, progressively more spermatozoa became capacitated such that at the end of the incubation, 40.9±0.9% was capacitated acrosome-intact and 29±2.5% was capacitated acrosome-reacted. In conclusion, the motility and velocity of Merino spermatozoa were similar among rams. There were differences among rams in the capacitation profile; however there was no significant effect of incubation time and dilution rate on the capacitation status. Keywords: Merino rams, spermatozoa, motility, velocity, capacitation in vitro Animal Production 14(2):104-110
The Influence of Climatic Factors on the Motility, Velocity and Morphology of Merino Rams Spermatozoa Ismaya, Ismaya; Summers, P
ANIMAL PRODUCTION Vol 8, No 3 (2006): September
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Penelitian ini bertujuan untuk mengetahui pengaruh bulan demikian pula perubahan musim (panjang hari, sinar terang, curah hujan, temperatur, penguapan dan kelembaban) terhadap motilitas, kecepatan dan morfologi spermatozoa domba Merino. Enam domba Merino jantan fertil digunakan dalan penelitian ini.  Sperma domba ditampung setiap bulan, mulai Juni 2002 sampai Mei 2003 dengan menggunakan elektroejakulator. Data kecepatan dan morfologi kepala spermatozoa didalam medium TALP dianalisis dengan menggunakan computer-aided semen analysis (CASA). Analisis varian, klasifikasi satu arah digunakan untuk mengetahui pengaruh kecepatan dan morfologi kepala spermatozoa. Total rerata VAP, VSL dan VCL selama setahun masing-masing adalah 75,40 um/detik, 64,00 um/detik dan 113,50 um/detik.  Total rerata STR, LIN, dan ELO masing-masing adalah 75,20%, 51,30%, 35,50%. Total rerata ALH, BCF dan AREA masing-masing adalah 5.2 µm, 17.0 Hz, dan 3.0 µm2. Sebagai kesimpulan, iklim berpengaruh terhadap kecepatan dan morfologi kepala spermatozoa domba Merino. (Animal Production 8(3): 152-159 (2006)  Kata Kunci : Spermatozoa domba Merino, Kecepatan, Morfologi
Co-Authors Ismaya Ismaya L Fiztpatrick, L