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All Journal Animal Production : Indonesian Journal of Animal Production
TR Tagama, TR
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Veterinary

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Effects of Adding Adenosine Triphosphate to Semen Diluter on Quality of Spermatozoa of Fat-Tailed Sheep Pramono, E; Tagama, TR
ANIMAL PRODUCTION Vol 10, No 3 (2008): September
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

The research aimed to investigate the effects of adding different levels of Adenosine Triphosphate (ATP) to semen diluter on quality of sperms of fat-tailed sheep. Treatments consisted of 5, 10 and 15 mg of ATP addition into semen diluter (Egg Yolk Sodium Citric). Results showed that treatments have no significant effects on spermatozoa motility, significant effects on spermatozoa viability, and high significant effects on spermatozoa mortality.  On average, sperm motility for control was 79.16 ± 3.99%; ATP 5 mg = 84.13 ± 5.36%; ATP 10 mg = 85.16 ±  4.54%; and ATP 15 mg = 85.33 ± 3.79 %; sperm viability for control was 25.28 ± 0.49; ATP 5 mg = 26.17 ± 1.53; ATP 10 mg = 27.56 ± 1.48; and ATP 15 mg = 28.34 ± 1.03 (hours); sperm mortality for  control was 12.91 ± 1.05%; ATP 5 mg = 15.59 ± 5.12%; ATP 10 mg = 21.95 ± 2.32%; and ATP 15 mg = 22.25 ± 1.83 %; and quality of sperm for control was 198.216 ± 5.65; ATP 5 mg= 206.312 ± 5.67; ATP 10 mg = 208.164 ± 5.14; and ATP 15 mg = 207.104 ± 5.90.  It can be concluded that addition of ATP into semen diluter was able to increase spermatozoa viability. (Animal Production 10(3): 151-156 (2008) Key Words: Fat-tailed sheep, spermatozoa, viability, diluter
The Influence of Type and Serum Concentration to Oocyte Maturation Level on Sheep In Vitro Wattimena, J; Tagama, TR; Hadisutanto, B
ANIMAL PRODUCTION Vol 8, No 2 (2006): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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The objective of the research was to know the effect of serum type and concentration on in vitro maturation ovine oocyte. The result was expected to give information about the effect of serum type and concentration on in vitro maturation ovine oocyte, in order to find an alternative serum to reduce the use of serum from pharmacy industry. The result of research shows that concentration and type of serum significantly (P<0,05) influenced the maturation rate germinal vesicle (GV), germinal vesicle breakdown (GVBD), metaphase-I (M-I) and metaphase-II (M-II) phase. The result of research shows that concentration and tyoe of serum has no significant (P>0,05) influence on cumulus expansion rate grade 0, 1 and 2. (Animal Production 8(2): 94-99 (2006) Key Words: Oocyte, Maturation, Sheep
Co-Authors B Hadisutanto, B E Pramono, E J Wattimena