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All Journal Animal Production : Indonesian Journal of Animal Production
DM Saleh, DM
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Veterinary

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Effect of Number of Spermatozoa, Oviduct Condition and Timing of Artificial Insemination on Fertility and Fertile Period of Kampung Rooster Spermatozoa Saleh, DM; Sugiyatno, Sugiyatno; Mugiyono, S; Siswadi, RW
ANIMAL PRODUCTION Vol 14, No 1 (2012): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract. This study was carried out to determine the optimum fertility and fertile period using the number of spermatozoa, oviduct condition and timing of insemination of native rooster spermatozoa. Ninety six commercial Isa brown pullets and nine kampung roosters were used in this study in a 3×2×2 factorial arrangement with one bird in a cage constituting a unit. The factor levels were the number of spermatozoa (50, 100 and 150 million/0.1 ml), oviduct condition (hard-shelled eggs and free hard-shelled eggs), and timing of artificial insemination (in the morning, at 7 AM and in the afternoon, at 4 PM).  The results showed that among the treatments there was no significant interaction to fertility and fertile period. Insemination with 50 million sperm number seemed to be the same result with the other 2 treatments. Oviduct condition had a highly significant difference on fertility and fertile period percentage, and timing of insemination did not differ between morning and afternoon.  In conclusion, the only oviduct condition (free hard-shelled eggs) was the best results for insemination in terms of fertility and fertile period of native roosters.  It is recommended that for the maximum fertility and fertile period, hens should be inseminated with 50 million spermatozoa, free of hard-shelled eggs and insemination performed in the morning or in the afternoon. Keywords: timing of artificial insemination, fertility, fertile period, semen dose, oviduct conditionAnimal Production 14(1):32-36, January 2012
The Effect of Adding Sodium-Potassium and Calcium-Magnesium to The Diet of Native Chicken Hen on Sex Ratio of Native Chicks Saleh, DM; Iriyanti, N
ANIMAL PRODUCTION Vol 12, No 1 (2010): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Thirtysix native hens were used to determine the effect of adding Sodium-Potassium and Calcium-Magnesium to the diet of Native Chicken hens on determination of native chick sexes. Fifteen hens (n=5; control, Na-K and Ca-Mg) were fed for 1 month and the other hens (n=6, for control, Na-K and Ca-Mg) were fed for two months, then  all hens were artificially inseminated, intra-vaginally, dose of 100 million perm cell per 0.1 ml, once a week for two weeks. On day 2 of insemination, all eggs were collected and evaluated daily for 14 days.  Every 5 days, the selected eggs were incubated for 7 days and followed by candling to assess fertility.  On day 21-22 of incubation, day old chicks were evaluated using vent sexing method to assess the sex ratio. Type of nutrition, fed at both for 1 month and two months had a similar results in fertility, hatchability and chick sex ratio:  62.79, 58.03, 59.52;  52.02, 50.93, 55.54 and 46.11, 51.89, 46.67 percent, respectively.  Feeding period of three type of diet value for fertility, hatchability and sex ratio is 59.19, 61.04; 53.61, 52.05, and 48.24, 47.86, respectively.  Our results suggest that different amount of ions in the diet of hens could not have a significant effect on the percent fertility, hatchability and sex ratio of chicks. (Animal Production 12(1): 12-15 (2010)Key Words : Na-K, Ca-Mg, fertility, hatchability, sex ratio, chicks
Effect of Artificial Insemination Time on the Fertility of Layer Hens Saleh, DM; Sugiyatno, Sugiyatno
ANIMAL PRODUCTION Vol 8, No 2 (2006): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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A study on the effect of AI time on the fertility of layer hens was carried out. Semen was collected from eight kampung roosters, with age ranging from 12-18 months.  Completely Randomized Design was used to analyze the data obtained.  Every eight hens (n=8) was inseminated intravaginally at 0-2 hrs, >2-4 hrs, > 4-6 hrs and >6-8 hrs after oviposition.  The results showed that insemination time did not affect  (P>0.05) the percentage of fertility from days 2 to 20.  However, the insemination time affected (P<0.05) the percentage of fertile eggs from days 2 to 9 and it did not affect (P>0.05) the mean duration of the fertile period.  In conclusion, the time of insemination at 0 – 2 hours after oviposition gave the best fertility.  It is recommended that for the best fertility flock inseminations should be performed at a time of 0-2 hours after an egg is laid every 9 days. (Animal Production 8(2): 83-87 (2006) Key Words: Kampung roosters, semen, fertility, fertile period, AI time
Effect of Glycerol Levels on the Motility and Fertility of Kampung Chicken Spermatozoa Frozen in Liquid Nitrogen Saleh, DM; Sugiyatno, Sugiyatno
ANIMAL PRODUCTION Vol 9, No 1 (2007): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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The use of various levels of glycerol as a cryoprotective agent for freezing kampung chicken semen was examined with respect to intravaginal insemination.  There was a significant (P<0.05) effect of various levels of glycerol (4, 8, 12 and 16 %) on motile spermatozoa, but there was no significant effect on fertility.  It was concluded that the use of glycerol for preservation of kampung chicken semen in liquid nitrogen gave satisfactory cryopreservative results on    motility of thawed kampung chicken spermatozoa, but failed to produce fertile eggs. (Animal Production 9(1): 45-48 (2007) Key Words: glycerol, kampung chicken, semen, fertility, motility, liquid nitrogen
Co-Authors N Iriyanti, N RW Siswadi, RW S Mugiyono, S Sugiyatno Sugiyatno