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All Journal Animal Production : Indonesian Journal of Animal Production
MA Mu’in, MA
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Veterinary

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Evaluation of Polymorphism of Leu/Val of Growth Hormone Gene in Friesian Holstein Cows at Breeding Centre of Dairy Cattle Baturraden Mu’in, MA; Zurahmah, N
ANIMAL PRODUCTION Vol 11, No 3 (2009): September
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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The Leu/Val polymorphism of the growth hormone evaluation was studied in 35 Friesian Holstein dairy cow at Baturraden Breeding Centre of Dairy Cattle. A 211 bp DNA fragment of GH gene spanning from the forth intron region (49 bp) to fifth of exon (162 bp) was amplified and digested with AluI restriction enzyme to identified polymorphism at this locus. Allele frequencies of variant Leu (leucine) and Val (valine) were 0.91 and 0.09, respectively. Selection to raising of Val allele frequencies have probably to increase of milk production at population of Friesian Holstein dairy cow at Baturraden Breeding Centre of Dairy Cattle. (Animal Production 11(3): 155-159 (2009) Key Words: gene, growth hormone, Leu/Val polymorphism, Friesian Holstein
Polymorphism of the Growth Hormone Gene and its Association with Growth Traits in Ongole Grade Crossed with Simmental Cattle Mu’in, MA; Astuti, M; Muladno, Muladno; Murti, TW; Artama, WT
ANIMAL PRODUCTION Vol 9, No 2 (2007): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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The present study was conducted to detect polymorphism of growth hormone (GH) gene in Ongole grade crossed with Simmental bull (SIMPO) and its association with growth traits. Blood samples of 62 cattle were taken from population of SIMPO cattle were located in the sub-province region of Bantul, Special Region of Yogyakarta. A 211 bp fragment of GH gene spanning from the forth intron region (49 bp) to fifth of exon (162 bp) was amplified and digested with AluI restriction enzyme to identified polymorphism at this locus. The resulted indicated that two genotypes LL and LV were found at the GH gene in SIMPO population cattle. The frequencies of L and V alleles were 0.82 and 0.18, respectively. In SIMPO calves, the average birth weight, 2 months body weight and daily body weight gains of LV genotypes were tend to higher than that of LL genotypes. (Animal Production 9(2): 53-58 (2007) Key Words : Gene, growth hormone, polymorphism, cattle, SIMPO
Identification of MspI Polymorphism in the Forth Intron of Chicken Growth Hormone Gene and Their Associations with Growth Traits in Indonesia Native Chickens Mu’in, MA; Lumatauw, S
ANIMAL PRODUCTION Vol 15, No 1 (2013): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract. The objective of this research was to identify MspI polymorphism in the forth intron of chicken growth hormone (cGH) gene and their associations with growth traits in native Indonesian chickens. A total of 72 Indonesia native chickens were genotyped for a locus in the forth intron of cGH gene (cGH-I4/MspI locus) by PCR-RFLP with MspI restriction enzyme. The result showed two genotypes in this locus: AA and BB, with the frequency of 90.28% and 9.72%, respectively. Based on body weight average, B allele had a beneficial effect in increasing the live body weight. The result of General Linier Models analysis indicated that the polymorphism of this locus had significant association (P<0.05) with body weight at 4 months of age and so did the daily gain between 2 to 4 months of age. Therefore, these results suggest that there is a possibility of cGH-I4/MspI locus acting as a genetic marker for growth traits of native Indonesian chickens, especially for body weight at 4 months and daily gain between 2 to 4 months of age. Keywords: polymorphism, cGH gene, growth traits, Indonesia native chickens. Abstrak.  Tujuan dari penelitian ini adalah untuk mengidentifikasi polimorfisme MspI intron ke-4 pada gen hormon pertumbuhan ayam (cGH) dan hubungannya dengan sifat pertumbuhan pada ayam asli Indonesia. Sebanyak 72 ekor ayam asli Indonesia diidentifikasi gentotipenya untuk lokus pada intron ke-4 dari gen cGH (cGH-I4/MspI lokus) dengan PCR-RFLP mengguakan enzim restriksi MspI. Hasil penelitian menunjukkan adanya dua genotipe pada lokus ini: AA dan BB , dengan frekuensi masing-masing 90.28 % dan 9,72 %. Berdasarkan rata-rata bobot badan, alel B memiliki pengaruh yang menguntungkan dalam meningkatkan bobot badan hidup. Hasil analisis General Linier Model menunjukkan bahwa polimorfisme lokus ini memiliki hubungan yang nyata (P<0,05 ) dengan bobot badan pada umur 4 bulan dan begitu pula dengan pertambahan bobot badan harian antara umur 2 sampai 4 bulan. Oleh karena itu, hasil ini menunjukkan bahwa ada kemungkinan cGH-I4/MspI lokus bertindak sebagai penanda genetik untuk sifat pertumbuhan ayam asli Indonesia, terutama untuk bobot badan pada umur 4 bulan dan pertambahan bobot badan harian ayam antara umur 2 sampai 4 bulan. Kata kunci: polimorfisme , gen cGH , sifat pertumbuhan , ayam asli Indonesia.MA Mu’in and S Lumatauw/Animal Production 15(1):1-7, January 2013
Co-Authors M Astuti, M N Zurahmah, N S Lumatauw, S TW Murti, TW WT Artama, WT