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All Journal Animal Production : Indonesian Journal of Animal Production
R Pratiwi, R
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Veterinary

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RFLP Marker Variation of Cytocrome b Gene and Genetic Relationship among Batur, Merino and Local Sheep Breeds Prayitno, Prayitno; Hartati, T; Pratiwi, R; Artama, WT
ANIMAL PRODUCTION Vol 13, No 3 (2011): September
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

RFLP analysis of mitochondrial DNA cytochrome b gene was conducted to determine the diversity, status and close genetic relationships in a population of Batur sheep with the Merino and local sheep breeds (Garut, Thin Tail and Fat Tail). The research used genomic DNA of 27 samples of Batur, 15 Merino, 17 Garut, 15 Thin Tails and 15 Fat Tails sheep. The PCR process used two types of 25 nucleotides primers. The PCR products were checked by using 2% agarose gel electrophoresis. The PCR DNA fragment was digested by using Hae III at 37 oC and incubated for 10 hours. Similarities and differences of cytochrome b gene RFLP bands between individual samples of one and across populations, genetic distance, and close genetic relationship, were identified. The PCR process of the cytochrome b gene metochondrial DNA of the 45 samples of sheep yielded 359 bp band types. The digestion (cutting) of the PCR products of mitochondrial DNA cytochrome b gene by using Hae III resulted in RFLP band profiles of 128 up to 231 bp polymorphisms of cytochrome b gene. Although the Hae III restriction enzyme recognized only one restriction site, however, between samples of Batur, Merino, Garut, Thin Tail, and Fat Tails, there were monomorphism and polymorphism Hae III loci.Key Words:  RFLP, cytochrome b gene, genetic markers, genetic similarity, Batur sheeAnimal Production 13(3):156-165 (2011) 
Genetic Relatedness Between Batur, Merino and Local Sheep Based on Random Amplyfied Polymorphism DNA Marker Prayitno, Prayitno; Hartatik, T; Pratiwi, R; Artama, WT
ANIMAL PRODUCTION Vol 13, No 1 (2011): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

RAPD analysis to determine the diversity, status and genetic close relatioship Batur with Merino, Garut, Thin Tail and Fat Tail sheep genomic DNA was used in 27 Batur, 15 Merino, 17 Garut, 15   Thin Tails and  Fat Tails animals. The process of RAPD-PCR used five primers of 10 nucleotides. PCR results were electrophoresed with 2% agarose gel. Identified similarities and differences between individual RAPD bands one and cross-sample of the population, genetic distance and closeness of genetic relationship. The process 89 sample  sheep RAPD-PCR generated of 4189 band from 100 to 1500 bp which consisted of 91 type. Batur sheep samples produced bands at most (1666 tape) and the lowest Fat Tails (493 bands). The number monomorfism of bands most of the Batur (891 bands) and the lowest Garut (170 bands), but the polymorphism  bands highest  of the Batur (775 bands) and the lowest Fat Tails (262 bands). Between individuals within populations have similar genetic Merino highest and the lowest Thin Tail. Cross-population genetic similarity is highest individuals in the population Batur and Merino, while the lowest Merino and Thin Tail. The closest genetic distance was Batur with Merino population and the most apart distant Merino with Thin Tail or Merino and Fat Tails. Batur sheep population has the closest genetic relationship with the Merino and the most apart distant is with Fat Tail. (Animal Production 13(1):30-38 (2011)Key Words: RAPD, genetic markers, genetic similarity, sheep
Co-Authors Prayitno Prayitno T Hartati, T T Hartatik, T WT Artama, WT