K. Wibisono
1. Research Center for Food Crops, Research Organization for Agriculture and Food, National Research and Innovation Agency (NRIA), Bogor, Indonesia. 2. Graduate School of Bogor Agricultural University (IPB University), Bogor 11680, Indonesia

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Elemental Mapping and Quantities in Different Soybean Seed Colors Using Micro X-Ray Fluorescence and Their Correlations with Germination K. Wibisono; W. Nurcholis
Atom Indonesia VOL 49, NO 2 (2023): AUGUST 2023
Publisher : National Research and Innovation Agency

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/aij.2023.1300

Abstract

Micro X-ray fluorescence (μ-XRF) possesses a powerful analytical technique able to detect macro- and micro-elements. Each plant variety has a unique elemental composition and important role in the germination process. The aims of this study were to (1) map the elements and quantities in the soybean seed coat and endosperm, (2) investigate how the various elements might mediate the inter-relationship or correlation between elements within soybean seed genotypes with different seed coat colors, and (3) investigate that the targeted morphological characteristics especially in germination would be affected by seed elements. A μ-XRF technique was used for the elemental analysis and quantification. Three genotypes of Indonesian soybean were used in this study: greenish, black, and yellowish. In this study, we found that the silicon (Si) and magnesium (Mg) elements have a significant correlation. The high quantity of Si element in the embryo axis has a positive correlation with root length. The high quantity of Mg element which is evenly distributed on the endosperm has a positive correlation with normal germination. Si and Mg elements in the seeds have a negative correlation with imbibition water absorption. Based on the comparison between the three genotypes, the black genotype was superior in terms of germination and higher Si and Mg elements. Thus, the Si and Mg elements can be used as a reference in determining superiority of genotypes at the germination stage.