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All Journal Biosaintifika: Journal of Biology & Biology Education
Agus Nuryanto
Faculty of Biology, Universitas Jenderal Soedirman, Indonesia
Agriculture, Biological Sciences & Forestry

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Morphological Variation of Blue Panchax (Aplocheilus panchax) Lives in Different Habitat Assessed Using Truss Morphometric Diah Mustikasari; Suhestri Suryaningsih; Agus Nuryanto
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.26593

Abstract

Blue panchax (Aplocheilus panchax Hamilton, 1822) lives in broad ranges of habitat from open waters to closed waters, including at ex-tin mining pits in Bangka Island, Indonesia. Variable habitats might cause morphological variations due to different ecological factors. This study aimed to assess the morphological variation of blue panchax collected from different habitats using truss morphometric characters. Fish samples (70 individuals) were collected from abandoned ex-tin mining pits of different ages and a river in Bangka Island. Twenty-nine truss characters were analyzed using the Kruskal-Wallis test and post hoc with Dunn’s test . The results showed that almost all of the body parts of blue panchax found in ex-tin mining pits and rivers were significantly different (p-value 0.05), except some truss characters of caudal peduncle. The post hoc of Dunn’s test showed a positive correlation between habitats and truss characters (adj. sig 0.05). Some truss characters of killifish were different between ex-tin mining pits with different ages chronosequence, and some other characters were different between pits and river.  This study provides the first data about the morphological variation of blue panchax in ex-tin mining pits of different ages. The data is valuable as a scientific basis of further utilization of ex-tin mining pits in the areas.
Barcoding of Fin Clip Samples Revealed High Exploitation of Plectropomus leopardus in Spermonde Archipelago Agus Nuryanto; Hendro Pramono; Kusbiyanto Kusbiyanto; Muhammad Iqbal Ghifari; Novi Andareswari
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i3.16142

Abstract

Spermonde Archipelago in South Sulawesi is among groupers sources in Indonesia for International Marine Live Trade. It was not much known which species are mostly exploited in those areas during the study because only fin clip samples could be obtained. Here we performed DNA forensic analysis to groupers fin clips collected from Spermonde Archipelago using barcode marker to know what species were exploited and which ones was the most exploited species. A total of 110 fin clip samples were obtained during the field trips. Sequencing of the cytochrome c oxidase 1 gene was successful for only 36 samples. Species determination was based on sequences similarity to conspecific sequence available in barcoding of life database (BOLD). BOLD similarity test placed the 36 fin clip samples into four different species, namely Cromileptes altivelis, Epinephelus ongus, Plectropomus leopardus, and Plectropomus maculatus. The K-2P taxonomic tree showed clear separation among species which was supported by high K2P genetic distances among species. Most of fin clips were identified as Plectropomus leopardus indicates that this species was the most exploited in Spermonde Archipelago. This proved that molecular identification not only can be used to biodiversity study in certain area but also in forensic analysis of a threatened wildlife. This study has important contribution for conservation effort of those groupers species, especially P. leopradus and provides new approach for taxonomist and conservation managers to identify samples, especially when only incomplete specimens are available.
Genetic Variation in Cytochrome b-Hinf1 and -Alu1 Gene Correlated to Body Size in Soang Gourami (Osphronemus goramy) from Single Spawning Agus Nuryanto; Nael Huda Qonita; Hendro Pramono; Kusbiyanto Kusbiyanto; Petrus Hary Tjahja Soedibja
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.9301

Abstract

Soang gourami fingerling shows variable body sizes eventhough resulted from single spawning. Differences in body sizes among individuals is assumed to be correlated to their genetic component which can be studied using cytochrome b gene PCR-RFLP marker. This study aimed to determine specific PCR-RFLP marker among different sizes of soang gourami collected from single spawning. Genomic DNA was isolated using Chelex method. Cytochrome b gene were amplified and digested using four restriction enzymes. Specific markers were analyzed descriptivelly based on DNA band pattern appear in agarose gel. The result showed that PCR-RFLP markers of Cytochrome b-HinfI of 315 bp, and 210 bp, and also Cytochrome b-AluI of 334 bp and 189 bp are specific markers for large individuals, whereas small individuals are characterized by having Cytochrome b- HinfI 366 bp, and 159 bp and Cytochrome b-AluI 525 bp fragments. It is observed that genetic variation of Cytochrome b-HinfI and -AluI markers are possitively correlated to body size in soang gourami fingerling. Therefore, both cytochrome b-HinfI and -AluI gene can be reffered as specific markers to differentiate among different sizes of soang gourami strain fingerling from single spawning. This result proved that genetic divergences among individuals can be related with certain quantitative characters, such size related. Therefore our study can contribute on fisheries development, especially by providing new technique for fingerling selection to obtain high quality fingerling and also provide new insight the application of molecular technique in fisheries. 
Morphological Differences between Chaetodon auriga and Chaetodon vagabundus based on Truss Morphometric Characters Rosewitha Anggraeni Privieshanti Hadi; Elly Tuti Winarni; Dian Bhagawati; Agus Nuryanto
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 3 (2021): December 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i3.31466

Abstract

Chaetodon auriga and Chaetodon vagabundus are popular marine aquaria fish from Cahetodontidae family. Both species show subtle morphological variations, especially in juvenile stages, which might lead to misidentification. Additional morphological characteristics, such as truss morphometric, are valuable characters for species identification in Chaetodontidae. This study aimed to evaluate morphological differences between Chaetodon auriga and C. vagabundus based on truss morphometric characters. The study is expected to provide new morphometric characters for Chaetodon auriga and C. vagabundus differentiation on the southern coast of West Java, Indonesia. Fish samples were collected from Ujunggenteng and Taman Manalusu Beach. The specimens were identified based on morphological characteristics and referred to the characters available in the literature. Truss morphometric characters were measured using callipers with an accuracy of 0.01mm and convert to ratio values to obtain constant value. The data were analyzed statistically using the Mann-Whitney test in SPSS software packages. The result showed that five truss morphometric characters were significantly different between C. auriga and C.vagabundus.  This study concluded that C. auriga and C. vagabundus could be differentiated using truss morphometric character. This study provides five new morphometric characters for species differentiation in fish species, especially between C. auriga and C. vagabundus.
Molecular Identification of Fish Larvae from East Plawangan of Segara Anakan, Cilacap, Central Java, Indonesia Agus Nuryanto; Hendro Pramono; Moh Husein Sastranegara
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 1 (2017): April 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i1.9191

Abstract

Correct identification of fish larvae from East Plawangan is very difficult to be done due to undefined of their morphology, while correct name is an important information for management of that area as spawning and nersery ground. Therefore, so far no scientific report on what species that utilize East Plawangan as their spawning and nursery ground. Cytochrome c oxidase 1 gene provide a precise tool for correct larvae identification. This study aims to test the accuracy of the cytochrome c oxidase 1 gene on fish larvae identification. The COI gene were amplified and sequenced. The homology of sequences were checked using Basic Local Alignment Search Tool (BLAST). Genetic distances were calculated with the help of MEGA 6 software. Taxonomic tree was reconstructed using neighbor-joining and maximum parsimony methods with 1000 bootstraps repeats. A sum of 24 morphotypes were identified, indicating high diversity of fish that utilize East Plawangan as their spawning and nursery ground. BLAST result showed that seven morphotypes were convincingly identified into species level, while three remaining morphotypes could only be identified at generic level. Taxonomic tree shows clear discrimination among morphotypes. This proved that cytochrome c oxidase 1 gene showed high accuracy on larval species discrimination.
Co-Authors Diah Mustikasari Dian Bhagawati Elly Tuti Winarni Hendro Pramono Kusbiyanto Kusbiyanto Moh Husein Sastranegara Muhammad Iqbal Ghifari Nael Huda Qonita Novi Andareswari Petrus Hary Tjahja Soedibja Rosewitha Anggraeni Privieshanti Hadi Suhestri Suryaningsih