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All Journal Biosaintifika: Journal of Biology & Biology Education
Loekas Soesanto
Faculty of Agriculture, Universitas Jenderal Soedirman
Agriculture, Biological Sciences & Forestry

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Raw Secondary Metabolites of Chitosan-enriched Pseudomonas fluorescens P60 to Control Corn Sheath Blight Loekas Soesanto; Clarissa Nadya Pradiptha; Endang Mugiastuti
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 1 (2021): April 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i1.28775

Abstract

Corn is a strategic cereal with economic value and always face the corn sheath blight in the field. An alternative safely and environmentally friendly disease control is the use of biological agent Pseudomonas fluorescens P60 that produced raw secondary metabolites. This study aimed to test the effectiveness of chitosan enriched P. fluorescens P60 raw secondary metabolites against leaf blight and to determine the effect on the growth of maize. The research was conducted at the Laboratory of Plant Protection and Experimental Farm, Faculty of Agriculture, Jenderal Soedirman University. In vitro test used a completely randomized design and in planta test used a randomized block design consisted of four treatments and six replicates. The treatments consisted of control and the secondary metabolites of P. fluorescens P60 enriched with chitosan 1, 2, and 3%. Variables observed were inhibition ability, chitinase and protease analysis qualitatively, incubation period, disease intensity, infection rate, AUDPC, crop height, crop fresh weight, and root fresh weight. The results showed that the secondary metabolites of P. fluorescens P60 enriched with chitosan 3% was effective in inhibiting the growth of Rhizoctonia solani as 63.3%, produced chitinase and protease, and could control the disease by delaying the incubation period as 79.05%, lowering the disease intensity as 68.68%, lowering the infection rate as 100%, and lowering AUDPC as 83.32%. The secondary metabolites of P. fluorescens P60 enriched with chitosan 3% was effective in improving plant growth by increasing plant fresh weight as 33.9% and root fresh weight as 43.09% compared to control. Chitosan could be used for improving effectiveness of antagonistic bacteria raw secondary metabolites. The raw secondary metabolites of bacterial antagonists could be improved their effectiveness against plant diseases by enrichment of chitosan.
Application of Bio P60 and Bio T10 in Combination Against Phytophthora Wilt of Papaya Loekas Soesanto; Kustam Kustam; Endang Mugiastuti
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 3 (2019): December 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i3.20389

Abstract

Papaya is one of the most widely cultivated horticultural plants. Phytophthora wilt is an important papaya disease which results in production losses. This research aimed to determine the effect of Bio P60 (raw secondary metabolites of Pseudomonas fluorescens P60) and Bio T10 (raw secondary metabolites of Trichoderma harzianum T10) application in combination on Phytophthora wilt and on growth of pepaya. The research was conducted on Californian pepaya farm, Linggasari Village, Kembaran District, Banyumas Regency. Randomized block design was used with six replicates and five treatments consisted of control (Mancozeb 80%), Bio T10 flush + Bio T10 spray, Bio T10 flush + Bio P60 spray, Bio P60 flush + Bio T10 spray, and Bio P60 flush + Bio P60 spray. Variables observed were percentage of healthy leaves, infection rate, number of healthy leaves, and number of healthy pepaya fruit. Result of the research showed that application of Bio P60 and Bio T10 effectively cured Phytophthora wilt with the percentage of healthy leaves as 69.19% compared to control. The combination was able to increase the number of healthy leaves the number of healthy papaya fruit. The novelty of this research is that raw secondary metabolites of biological agents proven to be able to overcome the papaya diseases, which so far cannot be solved. The implication for the development of science is one step ahead in overcoming plant diseases biologically by utilizing raw secondary metabolites. The benefits for the community can overcome papaya plant diseases organically, safely, and environmentally friendly, and inexpensive.
Co-Authors Clarissa Nadya Pradiptha Endang Mugiastuti Kustam Kustam